We narrowed to 19,001 results for: Tec

1. pAG32-L-IFP-F[2] (clon 3)

   Plasmid

   #52900

   Purpose

   Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   L-IFP-F[2]
   

   Use

   Vector for homologous recombination

   Promoter

   TEF promoter

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

2. Halo-Erk2 donor

   Plasmid

   #113104

   Purpose

   Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   N terminal Erk2 donor region including homology arms and HaloTag
   

   Use

   Synthetic Biology

   Available Since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXcX-3.6TPH-GAL4p65

   Plasmid

   #32573

   Depositor

   Sergey Kasparov

   Article

   Benzekhroufa et al BMC Biotechnol. 2009 Mar 19;9:23.

   Insert

   Gal4p65
   

   Use

   Adenoviral

   Expression

   Mammalian

   Promoter

   3.6TPH

   Available Since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC-DEST-R3R4(R)

   Plasmid

   #141010

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTYF-1xSYN-EGFP

   Plasmid

   #19973

   Depositor

   Sergey Kasparov

   Article

   Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

   Insert

   SYN-EGFP-WPRE (SYN1 jellyfish, Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-Endosomes-13

   Plasmid

   #56433

   Purpose

   Localization: Endosomes, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Endosomes (RHOB Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 23, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pUC-DEST-cjBLIMP1-Venus-HygTK

   Plasmid

   #141028

   Purpose

   Reporter integration

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   mVenus
   

   Expression

   Mammalian

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-puro-miR-10b sponge

   Plasmid

   #25043

   Depositor

   Bob Weinberg

   Article

   Ma et al Nat Biotechnol. 2010 Apr . 28(4):341-7.

   Insert

   miR-10b sponge
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

9. GGE142

   Plasmid

   #120735

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Metabolic marker for selection after transformation in Y. lipolytica (Marker_Suc2)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   Marker_Suc2
   

   Use

   Cloning/assembly plasmid

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDRFLIP42

    Plasmid

    #65518

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Tags

    6His-Cit-attR1 and attR2-mCer-cMyc

    Expression

    Yeast

    Promoter

    pPMA1

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDRFLIP35

    Plasmid

    #65513

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Tags

    6His-AFPt9-attR1 and attR2-TFPt9-cMyc

    Expression

    Yeast

    Promoter

    pPMA1

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDRFLIP32

    Plasmid

    #65511

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Tags

    6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

    Expression

    Yeast

    Promoter

    pPMA1

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB6578

    Plasmid

    #106130

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA NuiHA-loxP-nat-CUP1p

    Plasmid

    #131170

    Purpose

    PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Tags

    Nui-HA

    Expression

    Yeast

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTn7·xylS

    Plasmid

    #122591

    Purpose

    Plasmid for genomic integraction of the xylS regulator into the Tn7 insertion site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    xylS
    

    Use

    Synthetic Biology; Tn7 genomic integration

    Expression

    Bacterial

    Promoter

    Pm

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR1003

    Plasmid

    #71821

    Purpose

    Express Streptococcus pyogenes dCas9 (D10A, H840A)

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
    

    Use

    CRISPR

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEos2-Talin-H-18

    Plasmid

    #57422

    Purpose

    Localization: Talin Head Domain, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Talin H
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Talin Head Domain

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pGWB602-mCherry-sfGFP

    Plasmid

    #213860

    Purpose

    For Agrobacterium transformation. mCherry-sfGFP under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    mCherry-sfGFP
    

    Expression

    Bacterial and Plant

    Promoter

    CaMV 35S

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 FLIPE-600n

    Plasmid

    #13541

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E WT
    

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-neoCV

    Plasmid

    #73889

    Purpose

    fission yeast drug selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    neoCV
    

    Expression

    Bacterial, Mammalian, and Y…

    Promoter

    CMV

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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