167,642 results

1. SypHer

   Plasmid

   #48250

   Purpose

   pH sensor

   Depositor

   Nicolas Demaurex

   Article

   Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

   Insert

   SypHer
   

   Expression

   Mammalian

   Mutation

   C199S

   Promoter

   CMV

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pLV-Hygro-EF1A-EGFP-PARP1

   Plasmid

   #176146

   Purpose

   EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

   Plasmid

   #216152

   Purpose

   Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with cryptic exon and C-terminal FLAG
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AbVec1.1-IGKC

   Plasmid

   #80796

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hCMV IE1 gene promoter

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE30-His-CKM

   Plasmid

   #124134

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Creatine kinase (CKM Chicken)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJL1

   Plasmid

   #69496

   Purpose

   pJL1 expression vector with sfGFP

   Depositor

   Michael Jewett

   Article

   Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

   Insert

   Super folder GFP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2T-CMV-ABEmax-BlastR

   Plasmid

   #152989

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   ABE7.10-nSpCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. C-Terminal Split Cas9 with GyrA intein

   Plasmid

   #58694

   Purpose

   Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
   

   Use

   AAV and CRISPR

   Tags

   GyrA Csplit Intein and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. LBR-mCherry

   Plasmid

   #172446

   Purpose

   Expression of nuclear envelope protein LBR with a C-terminal mCherry tag

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   lamin B receptor (LBR Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-EGFP-RNF152-IRES-mScarlet-I

    Plasmid

    #215373

    Purpose

    Bicistronic expression of EGFP-tagged lysosome membrane protein RNF152, along with mScarlet-I expressed as an expression control.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    RNF152 (RNF152 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-hTipin (pc4732)

    Plasmid

    #216862

    Purpose

    Mammalian expression vector encoding GFP tagged human Tipin

    Depositor

    Cristina Cardoso

    Article

    Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

    Insert

    Tipin (TIPIN Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. T7-VEE-OKS-iM

    Plasmid

    #58972

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    26S subgenomic promoter and IRES

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Cre-IRES2-GFP

    Plasmid

    #26646

    Purpose

    Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

    Depositor

    Anjen Chenn

    Article

    Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    GFP and IRES2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAF0689 Ef1a-Pa01_attB-BFP acceptor

    Plasmid

    #193472

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB_TRE3G_dCas9-5xGCN4_Hygro

    Plasmid

    #235572

    Purpose

    Dox-inducible expression of dCas9-GCN4 & genomic integration

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    dCas9-5xGCN4
    

    Use

    CRISPR

    Mutation

    D10A; H840A

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid

    #112669

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Expression

    Mammalian

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Flag-IDH1-R132H

    Plasmid

    #62907

    Purpose

    Expresses R132 mutant IDH1 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

    Insert

    IDH1 (IDH1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deleted Arg132 (R132)

    Available Since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-SOCS1

    Plasmid

    #174571

    Purpose

    Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    HA-SOCS1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti UBC LysoFlux (HaloTag7-mGFP-Galectin3)

    Plasmid

    #215008

    Purpose

    Express a Lysophagy reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRRL_SRSF2_P95H_mCherry

    Plasmid

    #84023

    Purpose

    expression of SRSF2 mutant in mammalian cells

    Depositor

    Robert Bradley

    Article

    Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

    Insert

    SRSF2 (SRSF2 Human)
    

    Use

    Lentiviral

    Tags

    2TA-mCherry and Flag

    Expression

    Mammalian

    Mutation

    P95H

    Promoter

    PGK

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. EB3N-VVDfast-mCl3-iLID

    Plasmid

    #190165

    Purpose

    Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    EB3N-VVDfast-mClover3-iLID
    

    Tags

    -VVDfast-mClover3-iLID

    Expression

    Mammalian

    Mutation

    1-189 aa from EB3

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAS_4xMx PylT_FLAG-Mx PylRS

    Plasmid

    #140011

    Purpose

    Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. HA-14-3-3 zeta

    Plasmid

    #116888

    Purpose

    Expresses HA-tagged human 14-3-3 zeta in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Article

    Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

    Insert

    14-3-3 zeta (YWHAZ Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2

    Plasmid

    #111259

    Purpose

    mini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 site

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mTurquoise2
    

    Expression

    Bacterial

    Mutation

    mTurquoise2 is codon optimized for expression in …

    Promoter

    Pc

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. Joint Modular Cloning (JMC) Toolkit

    Plasmid Kit

    #1000000233

    Purpose

    The Joint Modular Cloning (JMC) Toolkit includes a modular cloning chassis and set of functional parts for assembling plant transformation vectors.

    Depositor

    Daniel Voytas

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

26. EGFP gRNA (BRDN0000563266)

    Plasmid

    #80036

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. pT3-EF1α-Dest

    Plasmid

    #133299

    Purpose

    The pT3-EF1α destination vector without any insert which can be used for LR reaction.

    Depositor

    Xin Chen

    Article

    Lee et al Hepatology. 2008 Apr;47(4):1200-10. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

    Plasmid

    #126552

    Purpose

    Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

    Depositor

    Jonathan Karn

    Article

    Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

    Insert

    HIV-1 vector pNL4-3
    

    Use

    Lentiviral

    Tags

    CD8a and GFP

    Mutation

    Delta gag/pol

    Promoter

    HIV LTR

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCRISPomyces-2BD

    Plasmid

    #234428

    Purpose

    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template

    Depositor

    Min-Kyu Oh

    Article

    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

    Insert

    Modified Cas9 with polyaspartate linking
    

    Expression

    Bacterial

    Mutation

    Linked polyaspartate using glycine-serine linker

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. SpRY-YE1-BE4max

    Plasmid

    #198554

    Purpose

    Expresses human codon-optimized SpRY-YE1-BE4max and blasticidin resistance: EFS promoter-SpRY-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpRY-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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