We narrowed to 14,304 results for: TIM;

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pROF417

Plasmid

#155335

Purpose

Constitutive expression LFY-VP16-2A-RLuc in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-LFY-VP16-NLS-2A-RLuc-Tnos

Use

Luciferase and Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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Twitch-1 pcDNA3

Plasmid

#49812

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-1

Tags

ECFP and cpCit174

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-SARS-CoV-2-E

Plasmid

#215814

Purpose

Expresses SARS-CoV-2 Envelope protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Envelope (E )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-M

Plasmid

#215813

Purpose

Expresses SARS-CoV-2 Membrane protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Membrane (M )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-S (D614G)

Plasmid

#215838

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMY-empty-MCS

Plasmid

#163351

Purpose

The empty pMY backbone with multiple cloning site

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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PKG I alpha CAT

Plasmid

#16392

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 alpha (PRKG1 Human)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain, S65D

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
Cry2L348F(1-531)-mCh-BAXS184E

Plasmid

#117239

Purpose

Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

Cry2531.L348F-mCh-BAXS184E

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Halo-Tev-Keap1

Plasmid

#62456

Purpose

His6-halo tagged human Keap1 protein for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Bacterial

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pN006

Plasmid

#188470

Purpose

Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

AsCas12a

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pmKate2-MS2coatProtein

Plasmid

#103833

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

mKate2-MS2 coat protein (cp )

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS4A

Plasmid

#120826

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS4A ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS

Plasmid

#191457

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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mEmerald-PCNA-19

Plasmid

#54221

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

Insert

PCNA-19 (PCNA Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMT2-HA-MKK3

Plasmid

#21580

Depositor

John Kyriakis

Article

Molnar et al J Biol Chem. 1997 May 16. 272(20):13229-

Insert

mitogen-activated protein kinase kinase 3 (MAP2K3 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
CRY2olig(FL)-mCherry

Plasmid

#60033

Purpose

Expresses a fusion of full length CRY2 (E490G) with mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2 E490G (deltaNLS) (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

E490G, NLS mutated

Promoter

CMV

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJT9R

Plasmid

#98305

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

TRPL3AcNES1>TRPL41B

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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CMV500 A-C/EBP

Plasmid

#33352

Depositor

Charles Vinson

Article

Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

Insert

C/EBP (Cebpa Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pCRU5-inGFPt

Plasmid

#60236

Purpose

HTLV-1 based transfer vector encoding gfp-turbo reporter gene interrupted by intron

Depositor

Dmitriy Mazurov

Article

Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

Insert

inGFPt

Use

Retroviral

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EBFP2-P2A-EGFP

Plasmid

#184938

Purpose

Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

EBFP2-P2A-EGFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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