We narrowed to 26,599 results for: vit;

1. IBMc097

   Plasmid

   #161945

   Purpose

   Contains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherry

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
   

   Expression

   Bacterial

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)MBII-85-5'/3'ss mut

   Plasmid

   #67645

   Purpose

   MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

   Insert

   MBII85 snoRNA
   

   Expression

   Mammalian

   Mutation

   5' and 3' splice sites mutations

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-h-mmPtch1-B

   Plasmid

   #120888

   Purpose

   Expresses Ptch1-B for function assays

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPK1E-1+t

   Plasmid

   #48147

   Purpose

   Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6P1‐Afu‐rnhBΔPIP

   Plasmid

   #108696

   Purpose

   For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   rnhB
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C-terminal truncation (7aa) removing PIP box

   Promoter

   tac

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1‐Afu‐rnhB D101N

   Plasmid

   #108695

   Purpose

   For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   rnhB
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   D101N catalytic site mutation

   Promoter

   tac

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOmpW

   Plasmid

   #112121

   Purpose

   Bacterial expression of outer membrane protein W (OmpW), wild type

   Depositor

   Karen Fleming

   Article

   Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

   Insert

   OmpW (ompW E. coli)
   

   Expression

   Bacterial

   Mutation

   N-terminal signal sequence not included and repla…

   Promoter

   T7

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. SaABE8e-dimer

   Plasmid

   #138501

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-nSaCas9
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-hSyntenin2b

   Plasmid

   #89437

   Purpose

   mammalian expression of EGFP tagged human Syntenin2b

   Depositor

   Fritz Rathjen

   Article

   Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

   Insert

   SDCBP2 (SDCBP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   *see below

   Promoter

   CMV

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. enAsABE8e-dimer

    Plasmid

    #138507

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-dAsCas12a (En)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-3xFLAG-Puro PAK5 E144K

    Plasmid

    #167193

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 E144K

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 E144K
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-3xFLAG-Puro PAK5 M173I

    Plasmid

    #167192

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 M173I

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 M173I
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFLAG-mISG15-4EHP

    Plasmid

    #17354

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    mISG15-4EHP (EIF4E2 Mouse, Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

14. FLAG-SLC30A10-N43H

    Plasmid

    #82344

    Purpose

    Expresses FLAG-tagged SLC30A10-N43H in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

    Insert

    SLC30A10 (SLC30A10 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N43H

    Promoter

    CMV

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-SLC30A10-D248A

    Plasmid

    #82346

    Purpose

    Expresses FLAG-tagged SLC30A10-D248A in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

    Insert

    SLC30A10 (SLC30A10 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D248A

    Promoter

    CMV

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL7.0: Venus-oLID-CAAX (from KRas4B)

    Plasmid

    #60412

    Purpose

    Mammalian Expression of Venus-oLID-CAAX

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-oLID-CAAX
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5 FRT/TO MycLAP-STIL ∆CC

    Plasmid

    #80268

    Purpose

    mammalian expression plasmid for MycLAP-STIL coiled-coil deletion

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Tags

    Myc-GFP

    Expression

    Mammalian

    Mutation

    missing conserved coiled-coil (amino acids 721-74…

    Promoter

    CMV

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-PB1-MontiRed-LOV2dark

    Plasmid

    #178874

    Purpose

    Mammalian expression of PB1-MontiRed-LOV2dark

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-MontiRed-LOV2dark
    

    Expression

    Mammalian

    Mutation

    LOV2: C450A, L514K, G528A, L531E, N538E

    Promoter

    CAG

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-fTCAP-GFP

    Plasmid

    #22916

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    fugu titan cap promoter driving GFP
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. myc-hIFITM3-F63Q

    Plasmid

    #104363

    Purpose

    Expresses human IFITM3-F63Q with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    mutated the following amino acid: F63Q

    Promoter

    CMV

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AB.pCCL.sin.cPPT.U6.miR-16-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46593

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-16 Decoy (Mir16-1 Mouse, Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pGex-3x Flag MKK3

    Plasmid

    #14814

    Depositor

    Roger Davis

    Article

    Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

    Insert

    MKK3 (MAP2K3 Human)
    

    Tags

    Flag

    Expression

    Bacterial

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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23. pRS316-RGR-GFP-mm

    Plasmid

    #51059

    Purpose

    Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    Yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MS2-adRNA (3'UAG)

    Plasmid

    #170128

    Purpose

    Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MS2-ADAR2(3'UAG)-MS2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 and mouse U6

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pILGFP86

    Plasmid

    #83543

    Purpose

    Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    RPL4A promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    RPL4A

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pENTR-GFP-Patronin CC+CKK

    Plasmid

    #59048

    Purpose

    for baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Tags

    eGFP

    Expression

    Bacterial and Insect

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pENTR-GFP-Patronin CC 534–1457

    Plasmid

    #59050

    Purpose

    for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Tags

    eGFP

    Expression

    Bacterial and Insect

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pT7-AsCas12a_crRNA-site3 (RTW549)

    Plasmid

    #160138

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pG.Lantern-SM50-GFP

    Plasmid

    #135593

    Purpose

    Expression of SM50 Promoter-GFP mRNA for microinjection to visualize SM50 promoter activity in S. purpuratus

    Depositor

    Koji Akasaka, Mamiko Yajima

    Article

    Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

    Insert

    SM50 promoter (SM50 S. purpuratus)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Green Lantern GFP

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28b+R3(R112C/T180C)

    Plasmid

    #108195

    Purpose

    Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C) fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRS316-RGR-GFP-mHDV

    Plasmid

    #51058

    Purpose

    Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    Yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pWP1_mFTCD

    Plasmid

    #102317

    Purpose

    expression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    Ftcd (Ftcd Mouse)
    

    Use

    Lentiviral

    Promoter

    EF1alpha

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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33. pBS-TRE-cofilin1(S3A)-P2A-mTurquoise2

    Plasmid

    #163610

    Purpose

    Expresses constitutively active cofilin and mTurquoise2 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    cofilin1 (Cfl1 Mouse)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTetR-BCD2-Ana GvpC

    Plasmid

    #153298

    Purpose

    Expresses wild-type gas vesicle protein C (GvpC) from Anabaena flos-aquae

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. D92_Ld_360170

    Plasmid

    #135905

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    hypothetical protein, conserved
    

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pM117: VEGFA array presgRNA

    Plasmid

    #166869

    Purpose

    U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    Human VEGFA targeting array presgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG-ChroME(F243Y)-mRuby2-ST

    Plasmid

    #109127

    Purpose

    Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChroME(F243Y)-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSFFV-Cx43

    Plasmid

    #69442

    Purpose

    mammalian expression of rat Cx43

    Depositor

    Richard Veenstra

    Article

    Veenstra et al Circ Res. 1995 Dec;77(6):1156-65.

    Insert

    Cx43 (Gja1 Rat)
    

    Expression

    Mammalian

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

39. 8xCTS1_BpiI-cloning-site_SV40polyA

    Plasmid

    #124547

    Purpose

    Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCASP-SptP120-NS1-HilA

    Plasmid

    #153327

    Purpose

    Triggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-NS1 Monobody
    

    Use

    Salmonella expression

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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