We narrowed to 34,868 results for: cas

1. pBiFC-flag-hCaspase 2 CARD-VN173

   Plasmid

   #49260

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 CARD (CASP2 Human)
   

   Use

   Tags

   Flag and split Venus N

   Expression

   Mammalian

   Mutation

   delete aa 123-435

   Promoter

   CMV

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pBiFC-flag-hCaspase 2 FL (C303S)-VN173

   Plasmid

   #49258

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 (CASP2 Human)
   

   Use

   Tags

   flag and split Venus N

   Expression

   Mammalian

   Mutation

   C303S

   Promoter

   CMV

   Available since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

3. XLone-Puro Cas13d-eGFP U6 SOX17 g2

   Plasmid

   #155188

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Insert

   Cas13d SOX17 gRNA2
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)

   Plasmid

   #191647

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletion

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

   Plasmid

   #107857

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 1) (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2T-CMV-evoCjCas9-eAID-BlastR

   Plasmid

   #194062

   Purpose

   Mammalian expression of evoCjCas9 eAID

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-eAID
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJEP303-pAAV-CMV-dSaCas9-VP64-pA

   Plasmid

   #113678

   Purpose

   A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   de-catalyzed SaCas9
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   NLS and VP64

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

   Plasmid

   #163545

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   POLD2-APOBEC1-UdgX-NG-nCas9-UdgX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

   Plasmid

   #161923

   Purpose

   To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   sgRNA targeting HSD17B11 exon 1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid

    #199221

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silient mutation at nucleic acid 177 C changed to…

    Promoter

    tight TRE promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

    Plasmid

    #68345

    Purpose

    A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

    Inserts

    puromycin
    hCas9
    

    Use

    CRISPR; Flp/frt

    Tags

    linked to red flouresent protein gene mKateII and…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCXLE-dCas9VPH-T2A-GFP-shP53

    Plasmid

    #102895

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9VPH-T2A-GFP-shP53
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-TREX1 g1 Cas9-T2A-mCherry

    Plasmid

    #164250

    Purpose

    Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgTREX1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC012 LwCas13a locus with nontargeting spacer

    Plasmid

    #91904

    Purpose

    LwCas13a locus into pACYC184 with nontargeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a with non-targeting spacer
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. SL2-sgTelo-MTSa/BFP/pdCas9-C1

    Plasmid

    #162760

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSDest2-NmeCas9-NLS-3XHA-NLS

    Plasmid

    #87448

    Purpose

    Mammalian expression of wild type Nme Cas9

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Nme Cas9
    

    Use

    CRISPR

    Tags

    3XHA, SV40 NLS, and cMyc-like NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+) Laccase2 MCS-ciRS7 Exon

    Plasmid

    #69900

    Purpose

    Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ciRS7 (stw, CDR1-AS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Equalizer-H plasmid with onboard mCherry cassette

    Plasmid

    #169733

    Purpose

    Equalizer-H plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

    Plasmid

    #91881

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)

    Plasmid

    #191635

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletion

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge