We narrowed to 10,277 results for: ARL

1. pXPR_BRD051-sgMLH1-2

   Plasmid

   #125777

   Purpose

   constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

   Depositor

   Francisca Vazquez

   Article

   Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

   Insert

   sgMLH1-2 (MLH1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCjedCas9

   Plasmid

   #172216

   Purpose

   Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells

   Depositor

   John van der Oost

   Article

   Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

   Insert

   catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
   

   Use

   Tags

   Histag

   Expression

   Mutation

   changed Asp 8 to Ala and His 559 to Ala

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV-KrasG12V/sgKras/Cre

   Plasmid

   #99854

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G12V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV-KrasWT/sgKras/Cre

   Plasmid

   #99848

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS-TRE-FHDnd1-Ubc-rtTA-I2G

   Plasmid

   #70076

   Purpose

   2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (Dnd1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Silent mutations are introduced to escape from RN…

   Promoter

   Tet responsible promoter

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3_N-DYK_MTHFD1-NES

   Plasmid

   #133022

   Purpose

   NES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

   Depositor

   Stefan Kubicek

   Article

   Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

   Insert

   MTHFD1 (MTHFD1 Human)
   

   Use

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-KrasG13C/sgKras/Cre

   Plasmid

   #99856

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G

   Plasmid

   #70075

   Purpose

   2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (Dnd1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Y102A. Silent mutations are introduced to escape …

   Promoter

   Tet responsible promoter

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-KrasG12R/sgKras/Cre

   Plasmid

   #99852

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G12R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Promoter

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAav-MDM2-PQS2-3xHA

    Plasmid

    #84886

    Purpose

    rAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

    Inserts

    MDM2 genomic sequence HR1 (MDM2 Human)
    MDM2 genomic insert HR2 (MDM2 Human)
    

    Use

    AAV

    Tags

    PQS2 3xHA

    Expression

    Mutation

    Promoter

    no

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-KrasG13D/sgKras/Cre

    Plasmid

    #99857

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-KrasG12S/sgKras/Cre

    Plasmid

    #99853

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pXPR_BRD003-sgWRN-EIJ

    Plasmid

    #125774

    Purpose

    constitutive expression of a guide RNA targeting an exon-intron junction of human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgWRN-EIJ (WRN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-KrasG12A/sgKras/Cre

    Plasmid

    #99849

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXPR_BRD003-sgPOLR2D

    Plasmid

    #125769

    Purpose

    constitutive expression of a guide RNA targeting human POLR2D (CRISPR positive control)

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgPOLR2D (POLR2D Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mdka (midkine-related growth factor)_R (OZ528)

    Plasmid

    #27205

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting mdka (mdka Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

17. mdka (midkine-related growth factor)_L (OZ527)

    Plasmid

    #27204

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting mdka (mdka Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

18. AAV-KrasG13V/sgKras/Cre

    Plasmid

    #99860

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CACNA1D (2of2) or cav1.3b_L (OZ547)

    Plasmid

    #28072

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting CACNA1D (2of2) or cav1.3b (cacna1db Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

20. CACNA1D (2of2) or cav1.3b_R (OZ548)

    Plasmid

    #28073

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting CACNA1D (2of2) or cav1.3b (cacna1db Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

21. AAV-KrasG13S/sgKras/Cre

    Plasmid

    #99859

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV-KrasG13A/sgKras/Cre

    Plasmid

    #99855

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AAV-KrasG13R/sgKras/Cre

    Plasmid

    #99858

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV

    Plasmid

    #74055

    Purpose

    retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc2, isoform C (NFATC2 AS 1-686 (N terminus deleted), Human)
    

    Use

    Retroviral

    Tags

    AVI-TEV and FLAG

    Expression

    Mammalian

    Mutation

    silent mutation A1723C in the sequence of NM_1730…

    Promoter

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. zebrafish similar to gpr151 (or gpcr-2037)_L (OZ535)

    Plasmid

    #27212

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

26. zebrafish similar to gpr151 (or gpcr-2037)_R (OZ536)

    Plasmid

    #27213

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

27. gRNA_Cloning Vector

    Plasmid

    #41824

    Purpose

    An empty gRNA expression vector, used to create a gRNA to a specific sequence.

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    AflII site for Gibson Assembly

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pANCH2-Nat

    Plasmid

    #87249

    Purpose

    gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    Nat
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Cdc2-HA

    Plasmid

    #1888

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdc2 (CDK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Mitochondrial cpGFP

    Plasmid

    #215711

    Purpose

    Expresses cpGFP in the mitochondria

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    cpGFP
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and mitochondrial localization sequence…

    Expression

    Mammalian

    Mutation

    circularly permuted GFP

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge