We narrowed to 22,359 results for: ESC

1. LI D-E C-NES c1

   Plasmid

   #54429

   Depositor

   Martin Parniske

   Article

   Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

   Insert

   NES of heat stable inhibitor (PKI)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET29b-ClbP-S95A

   Plasmid

   #48245

   Purpose

   Untagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assays

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Changed Serine 95 to Alanine

   Promoter

   T7

   Available since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDR-AmTrac-GS

   Plasmid

   #47769

   Purpose

   expresses AmTrac-GS in yeast

   Depositor

   Wolf Frommer

   Article

   De Michele et al eLife. 2013 Jul 2;2:e00800.

   Insert

   AmTrac-GS
   

   Use

   Tags

   mcpGFP

   Expression

   Yeast

   Mutation

   Promoter

   PMA

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pKK233-hCALML3

   Plasmid

   #47599

   Depositor

   Emanuel Strehler

   Article

   Rhyner et al Biochemistry. 1992 Dec 29;31(51):12826-3

   Insert

   CALML3 (CALML3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   contains 538 bp fragment

   Promoter

   trc

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNH052

   Plasmid

   #42158

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   YFP-RT-YFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNH054

   Plasmid

   #42160

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   mTFP1-RT-mTFP1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNH039

   Plasmid

   #42151

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   CFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNH058

   Plasmid

   #42161

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   TAP Tag::mTFP1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKB234.1

   Plasmid

   #170022

   Purpose

   Encodes LacI-controlled expression of surface-displayed hBD3 alpha-helix fragment and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   hBD3 alpha-helix
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and PvirK

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKB235.4

    Plasmid

    #170026

    Purpose

    Encodes LacI-controlled expression of surface-displayed CCL20 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    CCL20 Δ alpha-helix
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKB236

    Plasmid

    #170027

    Purpose

    Encodes LacI-controlled expression of surface-displayed TCP and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    TCP
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKB236.2

    Plasmid

    #170029

    Purpose

    Encodes LacI-controlled expression of surface-displayed TCP C-terminal alpha-helix fragment and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    TCP C-terminal alpha-helix
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNIR-FbALFA

    Plasmid

    #184678

    Purpose

    NIR-Fb to ALFA-tag

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbALFA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lamp1-mScarlet-I

    Plasmid

    #98827

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Lamp1
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. T444T-GFP(NCO)

    Plasmid

    #183972

    Purpose

    Enhanced RNAi clone for C. elegans knockdown of non-codon-optimized GFP

    Depositor

    David Matus

    Article

    Palmisano et al CourseSource, 2022

    Insert

    GFP (non-codon-optimized)
    

    Use

    RNAi

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBAD-Flp

    Plasmid

    #122969

    Purpose

    Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

    Depositor

    Lydia Freddolino

    Article

    Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

    Insert

    Flp recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Aug. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. LifeAct-mNeonGreen

    Plasmid

    #98877

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    LifeAct
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psiCHECK-2-XNS

    Plasmid

    #196655

    Purpose

    Promega's psiCHECK-2 Dual-Luciferase Reporter vector modified to allow the insertion of the target in opposite directions

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-shRNA-ctrl

    Plasmid

    #85741

    Purpose

    shRNA ctrl

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA ctrl
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-SFFV-GFP1-10

    Plasmid

    #80409

    Purpose

    Expresses GFP1-10 in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP1-10
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRN11

    Plasmid

    #84455

    Purpose

    shuttle plasmid for sarA P1-mCherry expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    mCherry
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTH100

    Plasmid

    #84458

    Purpose

    pJB38-NWMN29-30 + SarA_P1-sGFP-Term

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    SarA_P1-sGFP from pCM29
    

    Use

    Tags

    6xHis (on sfGFP)

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEVOL-pAzF

    Plasmid

    #31186

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

    Depositor

    Peter Schultz

    Article

    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

    Insert

    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32T, E107N, D158P, I159L, L162Q, D286R

    Promoter

    Available since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mTagBFP2-Endosomes-14

    Plasmid

    #55293

    Purpose

    Localization: Endosomes, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Endosomes (RHOB Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

25. Str-Ii_VSVG-SBP-EGFP

    Plasmid

    #65300

    Purpose

    synchronize trafficking of VSVG from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ATeam1.03-nD/nA/pcDNA3

    Plasmid

    #51958

    Purpose

    mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

    Insert

    ATeam1.03-nD/nA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV-CAG-ChR2-GFP

    Plasmid

    #26929

    Purpose

    AAV-mediated expression of ChR2-GFP for neuronal excitation

    Depositor

    Edward Boyden

    Article

    Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

    Insert

    ChR2
    

    Use

    AAV; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFA6a-link-yoEGFP-Kan

    Plasmid

    #44900

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBTK622

    Plasmid

    #180592

    Purpose

    tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

    Inserts

    tdk
    kanR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFA6a-link-yomEos2-Kan

    Plasmid

    #44944

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomEos2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEX6P1-GFP-Nanobody

    Plasmid

    #61838

    Purpose

    Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    GFP-Nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTn7-SCOUT11.G

    Plasmid

    #200999

    Purpose

    pLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23104

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-G-Flamp2

    Plasmid

    #192782

    Purpose

    green biosensor for cAMP in mammalian cells

    Depositor

    Jun Chu

    Article

    Liu et al Front Pharmacol. 2022 May 12;13:902290.

    Insert

    G-Flamp2
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Lamp1-mNeonGreen

    Plasmid

    #98882

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Lamp1
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1-GFP(1-10)

    Plasmid

    #70219

    Purpose

    Expresses GFP(1-10) in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Has Service

    Cloning Grade DNA

    Insert

    GFP(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHR-scFv-GCN4-sfGFP-GB1-dWPRE

    Plasmid

    #60907

    Purpose

    The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCas9CyMutL

    Plasmid

    #166981

    Purpose

    Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Inserts

    MutL (mutL )
    CymR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    pCymRC and pLacI

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Lamp1-mTurquoise2

    Plasmid

    #98828

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Lamp1
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-GR-ICUE2

    Plasmid

    #173017

    Purpose

    Improved green-red FRET based cAMP indicator.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    GR-ICUE2
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTn7-SCOUT12.G

    Plasmid

    #201000

    Purpose

    pLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23104

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge