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Showing: 541 - 570 of 159690 results

  2. pGEX4T1-Opt WWP1

    Plasmid
    #179327
    Purpose
    E. coli optimized expression of GST-fusion of human WWP1
    Depositor
    Arthur Haas
    Insert
    WWP1 (WWP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli
    Promoter
    Available since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179327-plasmid-map-sequence-id-358706

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  3. pAAV-iCre-2A-cherry

    Plasmid
    #182246
    Purpose
    Expresses iCre linked to mCherry. The promoter is a fragment of the hdc gene promoter which drives pan neuronal expression.
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    pan neuronal gene promoter (hdc) fragment driving iCre recombinase and 2A linked to mCherry
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    fragment of histidine decarboxylase gene promoter…
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    182246-plasmid-map-sequence-id-355958

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  6. pcDNA3.1(+)-AKAP95-FRB

    Plasmid
    #138254
    Purpose
    Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Jin Zhang
    Article
    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89
    Insert
    AKAP95-FRB (AKAP8 Human)
    Use
    Tags
    FRB rapamycin binding domain and HA epitope
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    138254-plasmid-map-sequence-id-307498

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  12. AICSDP-41: ATP2A2-mEGFP

    Plasmid
    #114405
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2
    Depositor
    Allen Institute for Cell Science
    Insert
    ATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114405-plasmid-map-sequence-id-220830

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  14. FRB_SCF2_LD6_CTEV

    Plasmid
    #58879
    Purpose
    MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58879-plasmid-map-sequence-id-94240

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  18. FKBP_SCF2_LD6_NTEV

    Plasmid
    #58878
    Purpose
    MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58878-plasmid-map-sequence-id-94239

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  19. RP4-6

    Plasmid
    #80385
    Purpose
    Helper plasmid for mobilisation derived from RP4. Spectinomycin/Streptomycin resistant
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Spectinomycin/Streptomycin
    Use
    Helper bhr plasmid for mobilizing other plasmids …
    Tags
    none
    Expression
    Mutation
    Promoter
    None
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  21. pCDNA3-LOV-VP16

    Plasmid
    #42499
    Purpose
    LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor
    Depositor
    Ricardo Dolmetsch
    Article
    Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d
    Insert
    FKF1 (N-LOV, G128D mutant) fused with VP16
    Use
    Tags
    F-type VP16 activation domain and HA tag
    Expression
    Mammalian
    Mutation
    G128D 1-168aa
    Promoter
    CMV
    Available since
    March 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    42499-plasmid-map-sequence-id-57336

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  24. p415 TEF Su9‐roGFP2‐Tsa2ΔCR

    Plasmid
    #83240
    Purpose
    The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For mitochondrial matrix expression.
    Depositor
    Tobias Dick
    Article
    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do
    Insert
    Su9-roGFP2-Tsa2dCr
    Use
    Tags
    roGFP2 fusion with Tsa2dCr with Su9 targetting se…
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83240-plasmid-map-sequence-id-164530

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  26. TFORF2019

    Plasmid
    #142312
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYBL2 (MYBL2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142312-plasmid-map-sequence-id-395783

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Showing: 541 - 570 of 159690 results