We narrowed to 34,835 results for: CaS;

1. NAX_CAGGS_CAS9_m4_Puro

   Plasmid

   #167871

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. M-SPcas

   Plasmid

   #48668

   Purpose

   Mammalian S. pyogenes Cas9 expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPhygCAS9

   Plasmid

   #63555

   Purpose

   Expresses CAS9 in Leishmania

   Depositor

   Greg Matlashewski

   Article

   Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

   Insert

   Humanized Streptococcus pyogenes Cas9 from PX330 (Addgene)
   

   Use

   Leishmania expression

   Tags

   flag

   Expression

   Mutation

   Promoter

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. DS-ST1casN-

   Plasmid

   #48659

   Purpose

   Bacterial nuclease-null ST1 Cas9 expression

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9, nuclease-null
   ST1 tracrRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D to A, DH to AA, N to A

   Promoter

   proC

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF_HA_CASP_sigma

   Plasmid

   #197614

   Purpose

   For expression of HA-tagged CASP sigma in E. coli

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   HA-tagged CASP sigma
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQCas12j2

   Plasmid

   #189780

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoter

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Insert

   Cas12j2
   

   Use

   CRISPR; Gateway compatible cas12j2 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. e-ciCas9_pcDNA5

   Plasmid

   #100553

   Purpose

   Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   e-ciCas9
   

   Use

   CRISPR; Frt, flp-in

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. DS-ST1cas

   Plasmid

   #48647

   Purpose

   Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   ST1 tracrRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   proC

   Available since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9m2

   Plasmid

   #47314

   Purpose

   Cas9 D10A+H840A

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   Cas9m2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

10. evoCas9

    Plasmid

    #138558

    Purpose

    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    evoCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    M495V, Y515N, K526E, R661Q

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9_mouse_Adar1_ccB

    Plasmid

    #158122

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9_mouse_Adar1_ccA

    Plasmid

    #158120

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. NAX_CAGGS_CAS9_m4_VPR_Hygro

    Plasmid

    #167883

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNOC_dCas9_sgRNA NC

    Plasmid

    #176259

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. NmeCas9_3XNLS_pMCSG7

    Plasmid

    #120078

    Purpose

    Bacterial expression of wtNmeCas9 with 3X NLS

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    NmeCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pxCas9CR4

    Plasmid

    #111656

    Purpose

    PCas9CR4 with the xCas9 mutations allowing NG PAM sites

    Depositor

    Christopher Reisch

    Insert

    TetR and xCas9
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    xCas9 mutations

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas5

    Plasmid

    #82378

    Purpose

    sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PJ23119

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. bbCas9pluspAAA

    Plasmid

    #82581

    Purpose

    The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA

    Depositor

    Ivo Huijbers

    Article

    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCasY1

    Plasmid

    #87687

    Purpose

    p15A plasmid expressing CasY1

    Depositor

    Jennifer Doudna

    Article

    Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

    Insert

    CasY1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. NAX_CAGGS_CAS9_EBFP2

    Plasmid

    #167857

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge