We narrowed to 6,021 results for: crispr cas9 expression plasmids

1. pX459V2.0-eSpCas9(1.1)+K810A

   Plasmid

   #108297

   Purpose

   pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-RSV-SpCas9

   Plasmid

   #85450

   Purpose

   Express SpCas9 in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pRSV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pRSV

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

   Plasmid

   #221385

   Purpose

   Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene

   Depositor

   Junwei Shi

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCgCas9_recT

   Plasmid

   #153337

   Purpose

   Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-SMVP-Cas9N

   Plasmid

   #80930

   Purpose

   Expresses Cas9N in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9-EcRT-Z3-Hyg

   Plasmid

   #232095

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Z3

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas9-EcRT-Z3-Nat

   Plasmid

   #232094

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Z3

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9-EcRT-Z3-Kan

   Plasmid

   #232096

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Z3

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1137=Hsp70Bb-Cas9[dsRed]

   Plasmid

   #149424

   Purpose

   The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Mutation

   Promoter

   Dmel Hsp70Bb

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. EC2_2_dCas9_VP64_sgRNA

    Plasmid

    #163707

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VP64+SV40 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-GFP (PX458)

    Plasmid

    #48138

    Purpose

    Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid

    #48140

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

    Plasmid

    #184294

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Adrb1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Grp

    Plasmid

    #184292

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Grp

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Grp
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Oprl1

    Plasmid

    #184291

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Oprl1

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Oprl1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Rxfp3

    Plasmid

    #184290

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Rxfp3

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Rxfp3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

    Plasmid

    #184295

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Adrb1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-Cas9-gRNA-TagBFP2

    Plasmid

    #124774

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-Cas9-gRNA-GFP

    Plasmid

    #124770

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NAX_CAGGS_CAS9_VPR_KanR neo

    Plasmid

    #167893

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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