179,539 results
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Plasmid#10977DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMOV10 (MOV10 Human)
TagsmycExpressionMammalianAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFLAG/HA-TNRC6B
Plasmid#10979DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTNRC6B (TNRC6B Human)
TagsFLAG and HAExpressionMammalianAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSUPER.PKCzeta.RNAi
Plasmid#10803DepositorAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FLAG.PKCzeta.T410E
Plasmid#10804DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPKC zeta (Prkcz Rat)
TagsFLAGExpressionMammalianMutationActivation loop active: T410E (Thr 410 to Glu)Available sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
1053 pBabe puroL Akt K179M T308A S473A
Plasmid#9013DepositorInsertAkt1 K179M T308A S473A (AKT1 Human)
UseRetroviralTagsHAExpressionMammalianMutationK179M T308A S473AAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pAAV-hSyn-DIO-EGFP (AAV9)
Viral prep#50457-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceSept. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMAL-7xHis-TEV-hexa
Plasmid#222868PurposeExpresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
Tags7xHis and MBPExpressionBacterialMutationC19V, L56V, C110V, C130S, S135G, S219V. Insert in…Available sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (AAV1)
Viral prep#62723-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (#62723). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] plasmid DNA. Cre-dependent expression of ChrimsonR-tdTomato under the control of the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagstdTomato (Cre-dependent)Available sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcVpx.myc
Plasmid#213388PurposeGenerate lentiviral vector which increases gene delivery into primary human myeloid cellsDepositorInsertSIVmac vpx (vpx Simian Immunodeficiency Virus)
UseLentiviralTagsmyc-6xHisExpressionMammalianPromoterCMVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6xHis-SNAP-PI(4,5)P2 Probe
Plasmid#211513PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteriaDepositorInsertPLCdelta1-PH
Tags6xhis-SNAPExpressionBacterialAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
B-95.ΔA
Bacterial strain#197933PurposeBL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acidsDepositorBacterial resistanceNoneAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Soma-jGCaMP8m (AAV9)
Viral prep#169257-AAV9PurposeReady-to-use AAV9 particles produced from AAV-hSyn-Soma-jGCaMP8m (#169257). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8m plasmid DNA. Syn-driven expression of soma-targeted calcium sensor jGCaMP8m. These AAV preparations are suitable purity for injection into animals.DepositorAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-mNeonGreen (AAV1)
Viral prep#99133-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-fDIO-mNeonGreen (#99133). In addition to the viral particles, you will also receive purified pAAV-CAG-fDIO-mNeonGreen plasmid DNA. CAG-driven, Flp-dependent mNeonGreen expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmNeonGreen (Flp-dependent)Available sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-hSyn1-GFP
Plasmid#177810PurposeEncodes a GFP driven by human synapsin I promoterDepositorInsertCopGFP inserted behind human synapsin I promoter (SYN1 Human)
UseLentiviralExpressionMammalianPromoterSyn1Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
mito-meGFP
Plasmid#172481PurposemeGFP targeted to the mitochondrial matrix with a human COX8 presequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMito (COX8A Human)
TagsmeGFPExpressionMammalianPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-S5E2-GCaMP6f (AAV PHP.eB)
Viral prep#135632-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gi2-CASE
Plasmid#168121PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at C112/E115 within GNAI2Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-hSyn-Soma-jGCaMP8m
Plasmid#169257PurposeAAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.DepositorHas serviceAAV1, AAV9, and AAV RetrogradeInsertSoma-jGCaMP8m
UseAAVTags6xHis-tagExpressionMammalianAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA-FLAG-METTL3-APPA
Plasmid#160251PurposeExpress catalytic inactive FLAG-METTL3 in human cellsDepositorInsertmethyltransferase like 3 (METTL3 Human)
TagsFLAGExpressionMammalianMutationchanges in aa 395–398, DPPW → APPAPromoterCMVAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBbS5a-Opto-Cre-Vvd-2
Plasmid#160400PurposeUpdated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.DepositorInsertOpto-Cre-Vvd-2
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterlacUV5Available sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-Gαi*-BERKY3
Plasmid#158198PurposeBRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
TagsmycExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOlyA(WT)
Plasmid#132391PurposeOstreolysin A for sensing sphingomyelin/cholesterol complexesDepositorInsertOstreolysin A wild-type
TagsHis6 tagExpressionBacterialMutationMutations: C62S, C94S, S151CAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP
Plasmid#156448PurposeFor transient expression of human CTCF tagged with eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman CTCF (CTCF Human)
ExpressionMammalianMutationnoneAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M13KO7-ΔPS-ΔgeneIII
Plasmid#134351PurposeHelper phage for M13 phage productionDepositorInsertHelper phage gene
ExpressionBacterialAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
psgRNA_EcoWG1
Pooled library#131625PurposeBacterial inhibition library targeting every small RNA and ORF of the E. coli MG1655 genome. This library has higher inhibition efficiency than the psgRNA library.DepositorExpressionBacterialSpeciesEscherichia coliUseCRISPRAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTol2-HuC(elavl3)-CaMPARI2
Plasmid#137185Purposepan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)DepositorInsertHuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
UseTol2 plasmid for zebrafishTagsFLAG, HA, mycPromoterHuC(elavl3)Available sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CyanoGate Kit
Plasmid kit#1000000146PurposeVectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.DepositorApplicationCloning and Synthetic BiologyVector typeBacterial ExpressionCloning typeGolden Gate (MoClo)Available sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM114
Plasmid#127211PurposeBeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDSDepositorInsertWUS2, IPT, sgRNA, Luc
ExpressionPlantMutationcodon optimized CDSsAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC3A2
Plasmid#132213PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC3A2 (SLC3A2 Human)
ExpressionMammalianAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
FRESCA-syntide
Plasmid#132415PurposeFRET-based sensor for CaMKII activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFRESCA-syntide
ExpressionBacterial and MammalianPromoterCMVAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC7A5
Plasmid#132197PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC7A5 (SLC7A5 Human)
ExpressionMammalianAvailable sinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
HR180-LGR5-GFP
Plasmid#129092PurposeKI EGFP with a puromycin/mRuby selection cassette into human LGR5 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) (AAV2)
Viral prep#121538-AAV2PurposeReady-to-use AAV2 particles produced from pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) (#121538). In addition to the viral particles, you will also receive purified pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) plasmid DNA. Synapsin-driven, HA-tagged hM4D(Gi) for neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsHAAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUGW mCherry-KASH
Plasmid#131505PurposemCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-inDepositorInsertmCherry_KASH
UseLentiviralExpressionMammalianPromoterhUBCAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-mScarlet
Plasmid#131001PurposeAAV vector to drive the expression of mScarlet under the control of human Synapsin promoterDepositorHas serviceAAV1 and AAV8InsertmScarlet
UseAAVPromoterhSynAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSGAb-spe
Plasmid#122000PurposeA sgRNA expression plasmid for genome editing in Acinetobacter baumanniiDepositorInsertnone
UseCRISPRAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL0284 (pQCascade_entry)
Plasmid#130635PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFD152
Plasmid#125546Purposederivative of pFD116 with an optimized RBS for E. coliDepositorInsertoptimized RBS
UseCRISPRPromoteranhydrotetracycline Ptet promoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFRT_TO_FlagHA_G3BP1
Plasmid#127106DepositorInsertG3BP1 (G3BP1 Human)
ExpressionMammalianAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB-rtTA
Plasmid#126034PurposePiggyBac cargo vector expressing rtTA. Also confers resistance to G418.DepositorInsertrtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
UsePiggybacExpressionMammalianPromoterhUbiCAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-fDIO-mNeonGreen
Plasmid#99133PurposeAn AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoterDepositorHas serviceAAV1InsertfDIO-mNeonGreen
UseAAVExpressionMammalianPromoterCAGAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBS_12x-601_MA1
Plasmid#114358Purpose12x Widom 601 nucleosome sequenceDepositorInsert12X601-MA1
UseUnspecifiedExpressionMammalianAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EB1-EGFP
Plasmid#118084PurposeExpresses EB1-EGFP in mammalian cellsDepositorAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TRE-fDIO-GFP-IRES-tTA
Plasmid#118026PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-fDIO-GFP-IRES-tTA
UseAAVMutationSee depositor comments belowPromoterTREAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
2Flag-pDEST-C
Plasmid#118372PurposeGateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2FlagExpressionMammalianAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VN-alpha Synuclein
Plasmid#89470Purposeexpresses human alpha Synuclein fused to the N-terminal part of VenusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSNCA (SNCA Human)
TagsN-terminal part of VenusExpressionMammalianPromoterCMVAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSGKP-spe
Plasmid#117234PurposeA sgRNA expression plasmid for genome editing in Klebsiella PneumoniaeDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pScI_dCas9-CDA-UL
Plasmid#108551PurposeBacterial Target-AID UGI-LVA vectorDepositorInsertdCas9-PmCDA1-UGI-LVA
TagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-mCherry
Plasmid#108685PurposeStrong CAG promoter with mCherry expression for transfection efficacy screeningDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry
ExpressionMammalianPromoterCAGAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by