We narrowed to 24,746 results for: CHI

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pJH2256

Plasmid

#132365

Purpose

Prgef-1 INS-6::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-6::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-6 (ins-6 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

Jan. 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Nano-lantern(ATP0)/pRSETB

Plasmid

#51989

Purpose

bacterial expression of a Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP0

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-TAL-NC2

Plasmid

#43855

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Tags

Flag and FokI nuclease

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGGS-TAL-NC2

Plasmid

#43856

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Tags

Flag and FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pJH2255

Plasmid

#132364

Purpose

Prgef-1 INS-4::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-4::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-4 (ins-4 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

Oct. 7, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Nano-lantern(ATP1)/pENTR1A

Plasmid

#51993

Purpose

Gateway entry vector for Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Use

Entry vector

Mutation

S257G in Rluc8

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pSG 416

Plasmid

#63863

Purpose

Detect translation initiation from AUA start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

AUA start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-125b-3p-Decoy.hPGK.GFP.WPRE

Plasmid

#46582

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-125b-3p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pGC4785

Plasmid

#113409

Purpose

Translation reporter with inducible translation coupling (low native GFP expression, high under coupling)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Insert

359_31121113_2

Use

Synthetic Biology

Tags

fused to codon deptimized sfGFP with Flexible lin…

Expression

Bacterial

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-128-Decoy.hPGK.GFP.WPRE

Plasmid

#46584

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-128 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pEF-DEST51 LacZ

Plasmid

#123292

Purpose

Mammalian expression of control truncated LacZ

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

LacZ

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pClneoFLAG mouse RASSF2

Plasmid

#37017

Depositor

Yutaka Hata

Article

Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

Insert

RASSF2 (Rassf2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

D72G and S127F

Promoter

CMV

Available Since

Aug. 13, 2012

Availability

Academic Institutions and Nonprofits only
Nano-lantern(Ca2+)_117/pRSETB

Plasmid

#51979

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=117nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-2GS

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern(Ca2+)_150/pRSETB

Plasmid

#51978

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=150nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-2G

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.GFP.miR-152-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85872

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-152-3p Sensor (MIR152 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac-LacI-LAP

Plasmid

#114034

Purpose

Production of baculovirus with LacI-LAP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP

Expression

Insect

Mutation

none

Promoter

CMV

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pATI (pKD-G7)

Plasmid

#62686

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123/amiR-eGFP419

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mEGFP

Plasmid

#105137

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P3nmt1-mEGFP

Tags

3nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAL942

Plasmid

#119721

Purpose

precursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 Cysteine

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

precusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C

Tags

none

Expression

Bacterial

Mutation

Alanine 248 to Threonine, Alanine 188 to Cysteine…

Promoter

T7

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-zEPLIN

Plasmid

#40957

Depositor

Elizabeth Luna, Scott Maul

Article

Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

Insert

EPLIN (lima1 Zebrafish)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pISH-Sncg

Plasmid

#105217

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Saraiva et al Sci Rep. 2015 Dec 16;5:18178. doi: 10.10

Insert

Sncg (Sncg Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEsg1-6K-Luc

Plasmid

#15394

Depositor

Shinya Yamanaka

Article

Amano et al BMC Dev Biol. 2006 Feb 28;6:11. doi: 10.

Insert

Upstream region of mouse Esg1 gene (Dppa5a Mouse)

Use

Luciferase

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pFETCh_SOX5

Plasmid

#86292

Purpose

Donor vector for 3' FLAG tag of human SOX5

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

SOX5 homology arms (SOX5 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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mEos2-Talin-ABM-10

Plasmid

#57421

Purpose

Localization: Talin Actin Binding Module, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin AMB

Tags

mEos2

Expression

Mammalian

Mutation

Actin Binding Module; I/LWEQ = 197 amino acids fr…

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
BB3rN_AH

Plasmid

#98556

Purpose

empty BB3 for insertion and overexpression of 7 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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hIRF-1 gRNA #409 (Sa)

Plasmid

#98134

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #409

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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hIRF-1 gRNA #351 (Sa)

Plasmid

#105283

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #351

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pATM (pKD-G11)

Plasmid

#62690

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123/amiR-eGFP419/tdTomato

Use

RNAi

Expression

Mammalian

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER-retro-puro-shNup88-HindIII

Plasmid

#87329

Purpose

To express shRNA against human Nup88

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup88

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p7139 pHAGE-P-CMVt-N-HA-GAW-PHKG2

Plasmid

#100159

Purpose

Expresses PHKG2

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

PHKG2 (PHKG2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p7138 pHAGE-P-CMVt-N-HA-GAW-PHKB

Plasmid

#100158

Purpose

Expresses PHKB

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

PHKB (PHKB Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_ZNF644

Plasmid

#86264

Purpose

Donor vector for 3' FLAG tag of human ZNF644

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF644 homology arms (ZNF644 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_SOX5_1

Plasmid

#86357

Purpose

Encodes gRNA for 3' target of human SOX5

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against SOX5 (SOX5 Human)

Use

CRISPR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Human NCKX2-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75202

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEST-p15A-OR2-OR1-Pr-UTR1-deGFPT500 (121p)

Plasmid

#70197

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_3_TOPO_TA-ms2-VP64

Plasmid

#79371

Purpose

Recruits VP64 to a compatible gRNA for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2-VP64

Use

CRISPR

Expression

Mammalian

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-16-2-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85876

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-16-2-3p Sensor (MIR16-2 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAct:MCP-VP64

Plasmid

#78904

Purpose

Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2:VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

March 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG 415

Plasmid

#63862

Purpose

Detect translation initiation from AUC start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

AUC start codon

Promoter

PtacI

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only
pSG 414

Plasmid

#63861

Purpose

Detect translation initiation from UUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UUG start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 431

Plasmid

#63866

Purpose

Detect translation initiation from GUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

GUG start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pBS Alpha 2 globin

Plasmid

#19231

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Alpha 2 globin (HBA1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pSB77

Plasmid

#19590

Depositor

Jay Keasling

Article

Bang et al Appl Environ Microbiol. 2000 Sep . 66(9)

Insert

thiosulfate reductase

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_LUC

Plasmid

#45290

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
p201N 1514

Plasmid

#47025

Purpose

Contains soybean miRNA miR1514 recognition sequence to produce siRNAs from 3' target sequences and induce RNA silencing

Depositor

Wayne Parrott

Article

Jacobs et al Plant Biotechnol J. 2015 Mar 27. doi: 10

Inserts

gma-miR1514 recognition sequence
nptII selectable marker

Use

RNAi

Promoter

GmUbi and StUbi-3

Available Since

Aug. 30, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
AB.pCCL.sin.cPPT.U6.miR-718-Decoy.hPGK.GFP.WPRE

Plasmid

#46632

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-718 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pGS1044

Plasmid

#63797

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection marker

Depositor

Monika Golas, Bjoern Sander

Article

Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1

Insert

3xFLAG followed by KlLEU2

Use

E. coli cloning vector

Tags

3xFLAG

Promoter

none

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.U6.miR-211-Decoy.hPGK.GFP.WPRE

Plasmid

#46600

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-211 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pHL1575

Plasmid

#53028

Purpose

PconNoHind:csrB, PLtetO-1:RBS(st3):csrD

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

csrB
csrD

Use

Synthetic Biology

Tags

RBS (st3)

Expression

Bacterial

Promoter

PconNoHind and pLtetO-1

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-ybbR-HIS-10FNIII(x4)-DocI

Plasmid

#58712

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

10FNIII(x4)-DocI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 9801 - 9850 of 24746 results

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