165,545 results

1. pLV hUbC-Cas9-T2A-GFP

   Plasmid

   #53190

   Purpose

   3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized Cas9 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT-TO-Nsp16

   Plasmid

   #157723

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp16 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGRG25

   Plasmid

   #16665

   Depositor

   Nancy Craig

   Article

   McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

   Insert

   mTn7::MCS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM-41-HsPABPC1_J

   Plasmid

   #146644

   Purpose

   Bacterial Expression of HsPABPc1

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

   Insert

   HsPABPc1 (PABPC1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two silent mutations compared to the sequence giv…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. HP17deltaP8

   Plasmid

   #235447

   Purpose

   Helper plasmid for regulated phage transfer

   Depositor

   Yolanda Schaerli

   Article

   et al Nat Commun 16, 3569 (2025)

   Insert

   Coding part of the M13KO7 helper phage genome, except gene VIII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PMXS-SLC1A3

   Plasmid

   #72873

   Purpose

   The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   SLC1A3 (SLC1A3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

7. hSyn-DIO-somBiPOLES-mCerulean (AAV5)

   Viral Prep

   #154951-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Promoter

   Syn

   Tags

   mCerulean (Cre-dependent)

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

8. pRRL-MHCK7-GCaMP6

   Plasmid

   #65042

   Purpose

   Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

   Depositor

   Nenad Bursac

   Article

   Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

   Insert

   GCaMP6
   

   Use

   Lentiviral

   Tags

   6xHis, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Mutation

   Promoter

   MHCK7

   Available since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTNT-B18R

   Plasmid

   #58978

   Purpose

   expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   B18R
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7/Sp6

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. RNAPII_CTD27-52

    Plasmid

    #98678

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3xFLAG-TYR

    Plasmid

    #32780

    Depositor

    Ruth Halaban

    Article

    Halaban et al J Invest Dermatol. 2002 Aug;119(2):481-8

    Insert

    Tyrosinase (TYR Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

12. mPlum-N1

    Plasmid

    #54629

    Purpose

    Localization: N1 Cloning Vector, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. tet-AID-PcrA M6-UGI-T2A-mTagBFP2-PGK-Puro-T2A-rtTA

    Plasmid

    #221234

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA M6-UGI-T2A-mTagBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTrichoGate-16

    Plasmid

    #160716

    Purpose

    T-nos Terminator bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    nopaline synthase terminator (T-nos) bordered by BsaI restriction sites
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Incorporation of BsaI restriction sites

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL103-SpCas9-FLAG-3XNLS

    Plasmid

    #182032

    Purpose

    Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP

    Depositor

    Steven Lin

    Article

    Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do

    Insert

    Streptococcus pyogenes cas9 (cas9 Synthetic)
    

    Use

    CRISPR

    Tags

    12X His tag, 3XNLS, FLAG, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. NM1-5S-tRNA-SgH

    Plasmid

    #128178

    Purpose

    Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    gRNA cloning cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    5S rRNA-tRNA(Arg)

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. OPA1-isoform1

    Plasmid

    #70173

    Purpose

    Mammalian expression of hsOPA1 isoform 1

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 1 (OPA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFJ782 - HygroR

    Plasmid

    #190933

    Purpose

    Hygromycin resistance co-injection marker

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Hygromycin resistance gene
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-0

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV3TAG8li-hSTT3A-3FLAG

    Plasmid

    #159140

    Purpose

    Hu STT3A -flag mammalian expression vector.

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    STT3 oligosaccharyltransferase complex catalytic subunit A (STT3A Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac1-6His-DHX9

    Plasmid

    #211440

    Purpose

    generation of Bacmid for insect cell expression of DHX9 N-terminally fused to 6XHis-Tag

    Depositor

    Helmut Pospiech, Frank Grosse

    Article

    Zhang et al J Biol Chem. 1997 Apr 25. 272(17):11487-

    Insert

    DHX9 (DHX9 Human)
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PiggyBac-TetO-Ascl1-neomycin

    Plasmid

    #176482

    Purpose

    TetO-Ascl1 in a PiggyBac vector with neomycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PCDNA3-PAR2-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113609

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    PAR2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAID5.3-C

    Plasmid

    #145813

    Purpose

    An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

    Type

    Empty backbone

    Use

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RFP-(SUMO)3

    Plasmid

    #127150

    Purpose

    Mammalian expression plasmid for mCherry-(SUMO)3 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    3 copies of human SUMO1 separated by (GGS)4 (SUMO3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Ultra-Exon1Q23-Myc-A

    Plasmid

    #110486

    Purpose

    Lentiviral vector to express wild-type N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged wild type huntingtin exon1 fragment with 23 repeats of Q)

    Depositor

    Baoji Xu

    Article

    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do

    Insert

    Homo sapiens huntingtin (HTT Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Short 3'UTR wild type huntingtin exon1 fragm…

    Promoter

    UbC

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q60

    Plasmid

    #111749

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q60 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a_Pro-IL-33

    Plasmid

    #214342

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL33 (IL33 Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HCT46_rAAV-eHGT_1188m-minBglobin-SYFP2-WPRE3-BGHpA

    Plasmid

    #223943

    Purpose

    Direct-expressing SYFP2 AAV virus

    Depositor

    Tanya Daigle, The Allen Institute for Brain Science

    Article

    Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    minBglobin

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HCT49_rAAV-3Xcore-eHGT_1139m-minBglobin-SYFP2-WPRE3-BGHpA

    Plasmid

    #223944

    Purpose

    Direct-expressing SYFP2 AAV virus

    Depositor

    Tanya Daigle, The Allen Institute for Brain Science

    Article

    Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    minBglobin

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRT801

    Plasmid

    #68112

    Purpose

    Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.

    Depositor

    Maggie Smith

    Article

    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

    Inserts

    aac3(IV)
    oriT
    attP
    int
    

    Use

    Streptomyces integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. EGFPC1-huNFATc1EE-WT

    Plasmid

    #24219

    Depositor

    Jerry Crabtree

    Article

    Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

    Insert

    NFATc1 (NFATC1 Human)
    

    Use

    Tags

    EE and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Fuw-dCas9-dead Tet1CD-P2A-BFP

    Plasmid

    #108246

    Purpose

    Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

    Insert

    dCas9-dead Tet1CD-HA-P2A-BFP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

    Promoter

    Ubc

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAVS1-TetOn-dCas9-KRAB

    Plasmid

    #115545

    Purpose

    Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

    Depositor

    Rene Maehr

    Article

    Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

    Plasmid

    #214885

    Purpose

    Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDY0216 PASTEv1 PE2-P2A-Bxb1

    Plasmid

    #179104

    Purpose

    PASTEv1 PE2-P2A-Bxb1

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-PE2-P2A-Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLL3.7-TNFa-shRNA3

    Plasmid

    #72598

    Purpose

    Express shRNA against mouse TNF-alpha

    Depositor

    Kazuhiro Oka

    Article

    Ogawa et al PLoS One. 2014 Mar 18;9(3):e92073. doi:

    Insert

    mouse TNF-alpha shRNA (Tnf Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pBGSA C5aR1-EGFP

    Plasmid

    #62612

    Purpose

    Constitutive expression of C5aR1-EGFP in mammalian cells

    Depositor

    Heini Miettinen

    Article

    Suvorova et al Traffic. 2005 Feb;6(2):100-15.

    Insert

    C5aR1 (C5AR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

    Plasmid

    #86419

    Purpose

    C-terminal PCR tagging module for yeast codon optimized split GFP1-10

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDEST/LIfeAct-mCherry-N1

    Plasmid

    #40908

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    LifeAct
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    No stop codon to create c-terminal fusion

    Promoter

    CMV

    Available since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA5/FRT/TO_Lifeact_HaloTag9

    Plasmid

    #175545

    Purpose

    Expression of HaloTag9 at the actin filaments of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge