Addgene

We narrowed to 14,780 results for: NOL

Showing: 6091 - 6120 of 14780 results

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pT7-TaGu-END-3xF-ORF

Plasmid

#238489

Purpose

For production of C-term flag-tagged TaGu EN-dead ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Expression

Mutation

DD1057/1070->AA

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLUEF1 Luc2-IRES-GFP

Plasmid

#224467

Purpose

Ef1a downstream of a ubiquitous chromatin opening element drives expression of Luciferase2 and IRES GFP

Depositor

Robert Kerbel

Article

Khan et al Cancer Res. 2025 Apr 14. doi: 10.1158/00

Insert

Luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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1010Av2

Plasmid

#125642

Purpose

Expresses FAR protein under 10xUAS control

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

FAR protein

Use

Tags

Expression

Insect

Mutation

Promoter

10xUAS

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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1010Bv2

Plasmid

#125643

Purpose

Expresses FAR protein under 10xUAS control

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

FAR protein

Use

Tags

Expression

Insect

Mutation

Promoter

10xUAS

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N73_eMagBF_V5

Plasmid

#235642

Purpose

Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5. No nuclear export sequence to allow nuclear localization.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5

Expression

Mammalian

Mutation

residues 1-73

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_Fbl_N73_eMagBF_V5

Plasmid

#235643

Purpose

Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, Fbl for targeting to nucleolus.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

Fbl-N73- and eMagBF-V5

Expression

Mammalian

Mutation

residues 1-73

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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PX459_L31_CRISPR_downstream_sgRNA

Plasmid

#235610

Purpose

Encodes CRISPR gRNA for cleaving downstream of insertion site at C terminus of mouse Rpl31

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus downstream sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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PX459_L31_CRISPR_upstream_sgRNA

Plasmid

#235609

Purpose

Encodes CRISPR gRNA for cleaving upstream of insertion site at C terminus of mouse Rpl31

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus upstream sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N73_eMagBF_V5_NES

Plasmid

#235614

Purpose

Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-73

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_BirA

Plasmid

#235634

Purpose

Encodes full-length BirA fused to a nuclear export sequence and V5. Used as a positive control in ALIBi experiments

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

BirA

Use

Tags

NES-V5

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N65_eMagBF_V5_NES

Plasmid

#235612

Purpose

Encodes ALIBi N-terminal fragment, residues 1-65, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-65

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N77_eMagBF_V5_NES

Plasmid

#235616

Purpose

Encodes ALIBi N-terminal fragment, residues 1-77, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-77

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N99_eMagBF_V5_NES

Plasmid

#235618

Purpose

Encodes ALIBi N-terminal fragment, residues 1-99, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Use

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-99

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN10-Pct3-(–)-Eldh1(cHis6)-noAccs(ElA2)

Plasmid

#233880

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh1 (Elen_0511 Eggerthella lenta)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN8-Pct3-(+)-Eddh(cHis6)-noAccs(ElA2)

Plasmid

#233879

Purpose

Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eddh (Elen_0501 Eggerthella lenta)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF26-Pct3-(+)-Eldh1(nHis6)(GpDSM19378)

Plasmid

#233874

Purpose

Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1 (GPA_25810 Gordonibacter pamelaeae DSM 19378)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF14-Pct3-ElVadh(cHis6)-Accs(ElDSM2243)

Plasmid

#233871

Purpose

Expresses His-tagged Eggerthella lenta DSM 2243 El Vadh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

El Vadh (Elen_0497 Eggerthella lenta)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF12-Pct3-(–)-Eldh1(cHis6)-Accs(ElA2)

Plasmid

#233870

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh1 (Elen_0511 Eggerthella lenta)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF10-Pct3-(+)-Eddh(cHis6)-Accs(ElA2)

Plasmid

#233869

Purpose

Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eddh (Elen_0501 Eggerthella lenta)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2092

Plasmid

#228342

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2093

Plasmid

#228343

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2094

Plasmid

#228344

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2095

Plasmid

#228345

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2096

Plasmid

#228346

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT1487

Plasmid

#228326

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT1980

Plasmid

#228334

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT1981

Plasmid

#228335

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2076

Plasmid

#228337

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2077

Plasmid

#228338

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT1434

Plasmid

#228317

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge