We narrowed to 15,226 results for: NOL

1. pColE1_sgRNA_M2S_AmpR

   Plasmid

   #119154

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   J23119

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB6682

   Plasmid

   #106152

   Purpose

   EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. TAL2236

   Plasmid

   #36687

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   CHD4-TALEN_Left (Chd4 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. EK0500 SFFV-mCherry-SGc(RPLP1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191184

   Purpose

   Expression of RPLP1 sensor in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:RPLP1.s1:EGFP-3xMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)

   Plasmid

   #191175

   Purpose

   Mammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   CMV-TO:mCherry:s70587:EGFP (frameshifted)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP is frameshifted

   Promoter

   CMV-TO

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ

   Plasmid

   #192396

   Purpose

   To act as a 2-input state of the AND gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ

   Plasmid

   #192391

   Purpose

   To act as a 2-input state of the OR gate.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEU3-NII-HaloLICNot

   Plasmid

   #140185

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, Halo-tag and NotI restriction site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   Halo

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBGSA FPR1

   Plasmid

   #62600

   Purpose

   Constitutive expression of FPR1 in mammalian cells

   Depositor

   Heini Miettinen

   Article

   Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

   Insert

   FPR1 (FPR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha

   Available since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pASH3 [pmyo-3::BeCNG1-YFP]

    Plasmid

    #168167

    Purpose

    Expression of BeCNG1-YFP in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

    Insert

    BeCNG1-YFP
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSiP1

    Plasmid

    #21952

    Depositor

    Klaus Rajewsky

    Article

    Xiao et al Nat Immunol. 2008 Apr . 9(4):405-14.

    Insert

    Pten (Pten Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2010

    Availability

    Academic Institutions and Nonprofits only

12. LZF70: Cre-off hSyn-QuasAr3-dark Citrine (FAS)

    Plasmid

    #104117

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    QuasAr3-dark Citrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRII-HSV2.3 TCR beta chain

    Plasmid

    #74077

    Purpose

    gBT-I TCR beta chain cDNA in pCRII

    Depositor

    Francis R. Carbone

    Article

    Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

    Insert

    TCR beta cDNA clone HSV2.3 (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLP675_L2_pV01_1I_vox-OCS-Rluc_TCTP_Vika_lacZ

    Plasmid

    #192427

    Purpose

    To act as the on state for testing of the alt Vika recombinase.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::vox-OCS-vox::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZ

    Plasmid

    #192432

    Purpose

    To act as a 2-input state of the NOR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-B3RT-Rluc-B3RT-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNB330

    Plasmid

    #60147

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    NLuc
    

    Use

    Tags

    Nuclear localization signal (NLS)

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUPD2 SV40-AcrIIA4 (GB3333)

    Plasmid

    #160605

    Purpose

    SV40-AcrIIA4 codon optimized for N.benthamiana

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

    Insert

    AcrIIA4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KG#470

    Plasmid

    #110930

    Purpose

    Expresses proteins pan-neuronally in C. elegans (entire nervous system)

    Depositor

    Kenneth Miller

    Article

    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

    Inserts

    rab-3 promoter
    unc-54 3' control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACBB-TetT-LVA

    Plasmid

    #32555

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    TetR
    

    Use

    Synthetic Biology

    Tags

    LVA tag

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pL59-Nat

    Plasmid

    #140465

    Purpose

    CRISPR/Cas9 engineering with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCellFree_G03 PRKCA

    Plasmid

    #67115

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PRKCA Protein kinase C alpha type (PRKCA Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

22. PB-HD118

    Plasmid

    #172655

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD118A/pegRNA-HD118B
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL3_4xUAS_CP-candidate_luc+

    Plasmid

    #125154

    Purpose

    STAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    4 x upstream activating sequence (UAS)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre

    Plasmid

    #192421

    Purpose

    To act as the 2-input state of the OR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-loxP-OCS-FRT-loxP::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

    Plasmid

    #87560

    Purpose

    Expresses aa397-423 of Flp recombinase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    FlpO aa397-423
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    PYL

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

    Plasmid

    #87559

    Purpose

    Expresses aa1-396 of Flp recombinase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    FlpO aa1-396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TAL2406

    Plasmid

    #36809

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    JAK2-TALEN_Left (Jak2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

28. EK0499 SFFV-mCherry-SGc(PPIB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191183

    Purpose

    Expression of PPIB sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:PPIB.s1:EGFP-3xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBM13-sd4U

    Plasmid

    #179691

    Purpose

    Bacterial expression of base editor

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q1310R, S1330I, T1380I

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EK0289 SFFV-mCherry-SG(s17d9b)SG-EGFP (FLP-IN)

    Plasmid

    #191142

    Purpose

    Expression of Sensor 2 (for EK0211) in mammalian cells; mCherry marker, EGFP output.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s17d9b:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge