We narrowed to 14,530 results for: TIM

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pET28a(+)-Gx-LB

Plasmid

#220126

Purpose

Expresses the 18-kDa Large BiT (LB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

Depositor

Maarten Merkx

Article

Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

Insert

Gx-LB

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHT-6xR.ads-PxylA-DI

Plasmid

#47386

Purpose

Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Inserts

ads
dxs
idi

Tags

6xR and His

Expression

Bacterial

Promoter

Pgrac and PxylA

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Pmel-1_TCRB

Plasmid

#175288

Purpose

Expresses Pmel-1 TCR beta chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor beta chain (Tcrb Mouse)

Use

Suitable for ivt

Expression

Mammalian

Promoter

CMV and T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Pmel-1_TCRA

Plasmid

#175287

Purpose

Expresses Pmel-1 TCRA alpha chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor alpha chain (Tcra Mouse)

Use

Suitable for ivt

Expression

Mammalian

Promoter

CMV and T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Sestrin2

Plasmid

#72593

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN2 (SESN2 Human)

Tags

HA

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-FLEX-GT

Plasmid

#26198

Depositor

Edward Callaway

Article

Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Enhanced green fluorescent protein, TVA (eGFP Mouse, TVA is a mouse codon optimized version of an avian gene.)

Use

AAV and Cre/Lox

Mutation

TVA mammalian codon optimized

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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SDHC-pHLuorin-sems

Plasmid

#107767

Purpose

labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

Depositor

Karin Busch

Article

Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

Insert

Subunit C of SDH fused to pHluorin (SDHC Human)

Tags

pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP gRNA (BRDN0000561167)

Plasmid

#80034

Purpose

3rd generation lentiviral gRNA plasmid targeting EGFP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV sMTase (eGFP)

Plasmid

#89930

Purpose

Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

dCas9-MC-IRES-MN

Tags

Flag and HA

Expression

Mammalian

Mutation

deactivated Cas9

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSLIK Tgfbr3-HA neo

Plasmid

#58508

Purpose

Lentiviral expression vector for an inducible HA-tagged mouse Tgfbr3

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

Tgfbr3_HA (Tgfbr3 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-HiBiT-CD9

Plasmid

#162592

Purpose

N-terminally HiBiT-tagged human CD9 under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

human CD9 (CD9 Human, human, Synthetic)

Tags

HiBiT

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSW003-Pc-mNeonGreen

Plasmid

#205018

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

Pc

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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CRY2PHR(W349R)-mCherry

Plasmid

#75370

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-498 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

W349R

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a TM-ER

Plasmid

#8353

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

T32A, S253A, S315A, mouse estrogen receptor (G525…

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
pAB1679 CMV-HIVNES-GS-Cas13bt3

Plasmid

#176317

Purpose

CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

human codon optimized Cas13bt3

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCA12-pHR-SFFV-BFP-B23

Plasmid

#199443

Purpose

Expression of BFP-B23 in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

B23 (NPM1 Human)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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Twitch-2B pRSETB

Plasmid

#48203

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2B

Tags

6xHIS, cpVenusCD, and mCerulean3

Expression

Bacterial

Promoter

T7

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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p415 TEF roGFP2‐Tsa2ΔCPΔCR

Plasmid

#83239

Purpose

The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

Insert

roGFP2-Tsa2dCpdCr

Tags

roGFP2 fusion with Tsa2dCpdCr

Expression

Yeast

Promoter

TEF

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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GST-BHMT-I/G

Plasmid

#104442

Purpose

Autophagy cargo assay

Depositor

Carol Mercer

Article

Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

Insert

BHMT (BHMT Human)

Tags

GFP-myc GFP is from Aequorea victoria and GST

Expression

Mammalian

Mutation

N43A in GST (in a potential KFERQ-like sequence).…

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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6691 Bicistronic_ires_puro

Plasmid

#64335

Purpose

This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Type

Empty backbone

Use

Retroviral

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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