We narrowed to 33,148 results for: Eng;
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Plasmid#229945PurposeBacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)DepositorInsertluxOFP
TagsHis-tagExpressionBacterialPromoteraraBADAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cytoFLARE1.0/1.1-TF
Plasmid#234516PurposeTranscriptional reporter for detecting calcium transients in neuronal cultures (transcription factor)DepositorInsertERT2-MK2-hLOV-TEVcs-FLAG-tTA
UseAAVExpressionMammalianAvailable SinceApril 30, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMC-1-StUbi-6
Plasmid#197710PurposePhytobrick (MoClo) Level 0 PartDepositorInsertStUbi promoter
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT138b-proB
Plasmid#138515PurposeP3fDepositorInsertT7-RNAP
UseSynthetic BiologyAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VCre
Plasmid#89575PurposeExpresses VCre under pCAGDepositorInsertVCre recombinase
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb
Plasmid#175534PurposeExpression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag7-myc_PDGFRtmb
ExpressionMammalianAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV-EF1s HA.NLS.Sce(opt)
Plasmid#31479DepositorInsertSFFV/Ef1s HA NLS I-SceI
UseLentiviralTagsHA and NLSMutation"opt" indicates the coding sequence has…Available SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-BCCP-PupE
Plasmid#113403PurposeExpresses BCCP-PupE in bacteriaDepositorInsertBCCP-PupE
TagsGSTExpressionBacterialPromotertacAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIV297
Plasmid#204820PurposeAMA1 plasmid with amdS selection markerDepositorInsertamdS
UseFungal expressionExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans trpC promoterAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD441_WT_hi
Plasmid#128857PurposeHigh copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
ExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-luxKate
Plasmid#229946PurposeBacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)DepositorInsertluxKate
TagsHis-tagExpressionBacterialPromoteraraBADAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D-cyto-Ruby3-iATPSnFR1.0
Plasmid#102554Purposeexpresses cytoplasmic ATP sensor in astrocytes with red reference proteinDepositorInsertmRuby3-iATPSnFR1.0
UseAAVAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330S-5
Plasmid#58781PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
p32Lb
Plasmid#158154PurposeConstruct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_210d
Plasmid#91109PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: noneDepositorTypeEmpty backboneExpressionPlantPromoter35SAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
peGFP-N1-SHB
Plasmid#139605PurposeExpresses eGFP-SHB fusion protein in mammalian cellsDepositorAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
FnR9-10
Plasmid#182567PurposeHuman fibronectin fragment, type-III repeats 9-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 9-10
TagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379AAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTarget12f1
Plasmid#171183PurposeConstitutive expression of sgRNA without donor DNADepositorInsertsgRNA scaffold in the CRISPR/Cas12f1 system
UseCRISPRExpressionBacterialPromoterJ23119(SpeI) promoterAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDD285
Plasmid#66826PurposemKate2^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmKate2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized mKate2ExpressionWormAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-ald*-alaE
Plasmid#134939PurposeLow phosphate inducible alanine dehydrogenase and alanine exporterDepositorInsertalanine dehydrogenase and L-alanine exporter
ExpressionBacterialPromoteryibDAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0679
Plasmid#149689PurposeBacterial expression of SENP_EuH proteaseDepositorInsertSENP_EuH protease
TagsHis14-tag and Tev protease cleavage siteExpressionBacterialAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
ykgMO-IGS-sfGFP
Plasmid#207231PurposeCalprotectin sensing promoter with sfGFP reporterDepositorInsertykgMO-IGS
UseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
ykgMO-IGS-LuxCDABE
Plasmid#207232PurposeCalprotectin sensing promoter with luciferase reporterDepositorInsertykgMO-IGS
UseLuciferase and Synthetic BiologyExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-IPT-9
Plasmid#197746PurposePhytobrick (MoClo) Level 0 PartDepositorInsertIsopentyl transferase (IPT)
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.IP
Plasmid#79472PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, IRES PuromycinDepositorTypeEmpty backboneUsePromoterless gateway destination vectorPromoternoneAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_201
Plasmid#91105PurposeTransformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDVDepositorTypeEmpty backboneExpressionPlantPromoterNo PromoterAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFlag-CMV2-Smyd2
Plasmid#13521DepositorAvailable SinceFeb. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_NES_HaloTag9
Plasmid#175537PurposeStrict cytosolic expression of HaloTag9 in mammalian cellsDepositorInsertNES-HaloTag9
ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-AsCas12j-2
Plasmid#191655PurposeAll-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2DepositorInsertAsCas12j-2
ExpressionBacterialPromoterrpsL(XC)-BbsIAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2-STOP-dsRed
Plasmid#60944PurposeDrosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.DepositorInsertdsRED
UseUnspecifiedPromoter3XP3Available SinceNov. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpaCas12f1-gRNA_MS13
Plasmid#190877PurposeThe plasmid of CRISPR/SpaCas12f1(Syntrophomonas palmitatica Cas12f1) for genome editing in human cellsDepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH2.4_mCherry-MMLV_Psi
Plasmid#205522PurposeExpress mCherry-Psi (export tag consisting of MMLV Psi)DepositorInsertmCherry
ExpressionMammalianMutationWTPromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCL28-decoder
Plasmid#159450PurposeInsert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.DepositorInsertPlexAtet-LexA-ER-B112-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPlexAtetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL66-decoder
Plasmid#159453PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ143-ZmUbi-tRNA
Plasmid#158400PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ133-tRNA2.0; assembly of 3 gRNAsDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterMaize ubiquitin 1Available SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_212
Plasmid#91111PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCVDepositorTypeEmpty backboneExpressionPlantPromoter35SAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB8644
Plasmid#126919PurposePlasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYBDepositorInsertXdCrtI and HMG1
ExpressionYeastAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUM
Plasmid#133130PurposeA recombinant plasmid expressing fum (fumarase) geneDepositorInsertfumarase gene
ExpressionBacterialPromoterPEP-carboxykinase promoterAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
MK1283
Plasmid#71428PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709DepositorInsertMixed Feedback Loop version of the UBER system
MutationT7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330S-6
Plasmid#58782PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mClover3
Plasmid#133453PurposeExpresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mClover3
TagsGSGSGSGS linker and His6ExpressionBacterialPromoterT7Available SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF6a-FKBP-CD28
Plasmid#113401PurposeExpresses FKBP-CD28 in mammalian cellDepositorInsertFKBP-CD28
ExpressionMammalianPromoterEF-1αAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
LRP6-pCS2-VSVG
Plasmid#27282DepositorInsertlow density lipoprotein receptor-related protein 6 (LRP6 Human)
UseXenopus injectionTagsVSVGAvailable SinceMarch 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAMP-CY1_NF1 T1 (+minintr)
Plasmid#119204Purposetransfer vectorDepositorInsertNF1 type 1 mini-gene (NF1 Human)
UseTransfer vectorAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-flex-oChIEF-citrine
Plasmid#50973Purposeflexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITRDepositorInsertChIEF-citrine
UseAAVTagscitrineMutationinverted and inserted between two lox sitesPromoterhuman synapsin promoterAvailable SinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-N-Tandem-mCherry-EGFP
Plasmid#123216PurposeN-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTagsEGFP and mCherryExpressionMammalianPromoterCMVAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-Gal4
Plasmid#53366Purposefull-length native Gal4 with stop in Gateway middle entry cloneDepositorInsertnative (full-length) Gal4
Promoterno promoterAvailable SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_260d
Plasmid#91126PurposeTransformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: noneDepositorTypeEmpty backboneExpressionPlantPromoterPvUbi2Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-secAnxaV-NS
Plasmid#67718PurposeGateway entry clone containing secreted human AnxaV with no stop codonDepositorInsertsecAnxaV-NS
ExpressionMammalianAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-oriT
Plasmid#32556DepositorInsertoriT
UseSynthetic BiologyExpressionBacterialPromoterN/AAvailable SinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only