We narrowed to 5,627 results for: mcs
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Plasmid#27437DepositorInserthistone-GFP-2A-TVA receptor-2A-rabies glycoprotein
UseAAV; Adeno-associated viralMutationN/AAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-mCherry-GPI
Plasmid#65299Purposesynchronize trafficking of mCherry-GPI from the ER (RUSH system)DepositorInsertGPI anchor
UseLentiviralTagsIL-2 Signal Sequence and Streptavidin Binding Pro…PromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SypHer2-DAAO-NES
Plasmid#119184PurposeFusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.DepositorInsertSypHer2-DAAO-NES
UseAAVTagsNESExpressionMammalianPromoterCMVAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV
Plasmid#91719PurposeAAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2DepositorInsertmEGFP-P2A-paAIP2(R5AR6A)
UseAAVPromoterCaMKII 1.3Available SinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRAN473
Plasmid#120813PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target proteinDepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
ExpressionBacterialPromoterPtetAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI H2B-RFP
Plasmid#92398PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.DepositorInsertIRES H2B RFP
ExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Hph
Plasmid#188741PurposepUC19 plasmid (MCS intact) with Hygromycin selection markerDepositorInsertHygromycin
UseFilamentous fungal expressionPromotergpdAAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGoldenGreenGate-M (pGGG-M)
Plasmid#165422PurposeA Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).TypeEmpty backboneUseSynthetic Biology; Level 2 golden gate (moclo) du…PromoterContain GG cassette and MCS with LacZ for blue/wh…Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVH1002-Tier2(pUC ori)
Plasmid#169600PurposeTier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).DepositorTypeEmpty backboneExpressionMammalianAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM123: pAAV-EFS-CasRx-control presgRNA
Plasmid#166871PurposeAAV vector for expressing CasRx and control presgRNA for RNA-editingDepositorInsertsU6-control presgRNA
RfxCas13d
UseAAV and CRISPRTagsHA and NLSExpressionMammalianPromoterEFS and U6Available SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_neomycin
Plasmid#65307PurposeLuminal ER hook only (RUSH system)DepositorInsertStreptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
UseLentiviralTagsKDEL motif and Signal SequencePromoterCMVAvailable SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-DeltaFosB
Plasmid#68545PurposeAAV expression of DeltaFosBDepositorInsertDeltaFosB-IRES-GFP
UseAAVExpressionMammalianMutationtruncated splice variant of FosBPromoterCMVAvailable SinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-dnPERK-IRES-eGFP
Plasmid#36954DepositorInsertdnPERK (EIF2AK3 Human)
UseRetroviralExpressionMammalianMutationdeletion of aa592-1061 to create dominant negativePromoterCMVAvailable SinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFH2.155_LV-Puro-T2A-mTagBFP2
Plasmid#205553PurposeExpress puromycin resistance marker and mTagBFP2DepositorInsertPuro-T2A-mTagBFP2
UseLentiviralExpressionMammalianMutationWTPromoterEF1Available SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMuLE (R4-R3) PRB
Plasmid#206164PurposepMuLE R4-R3 entry plasmid containing human full length PRB gene, for 3-component Gateway assemblyDepositorAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI H2B-GFP
Plasmid#92399PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.DepositorInsertIRES H2B EGFP
ExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-EGFP-Ecadherin
Plasmid#65290PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
UseLentiviralTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…PromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol2-nbt-Electra2-P2A-mScarlet
Plasmid#196114Purposezebrafish expression of nbt-Electra2-P2A-mScarletDepositorInsertnbt-Electra2-P2A-mScarlet
UseZebrafish expressionAvailable SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-tdTomato-cNLS
Plasmid#138532PurposeLentiviral inducible expression of tdTomato with nuclear localization signalDepositorInserttdTomato
UseLentiviralTags3x SV40 cNLSExpressionMammalianPromoterTRE-CMVAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-iCreV-4x6T
Plasmid#196412PurposeAstrocytic expression of light-inducible iCreV recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertiCreV
UseAAV; Astrocyte-selectiveExpressionMammalianPromoterCAG and GfaABC1DAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only