We narrowed to 24,987 results for: Nov;
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Plasmid#164877PurposeBFP-preSTOP-GFP reporterDepositorInsertBFP-preSTOP-GFP
ExpressionMammalianAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
LLP783_SpyCatcher-DNMT3A-CO
Plasmid#211776PurposeSpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavudin-DNMT3A
Tags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP618_αGCN4-KRAB-CO
Plasmid#211782PurposeSunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertaGCN4-mCherry
Tags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect mCherr…PromoterpEF1a and pSV40Available SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP617_αGCN4-mCherry
Plasmid#211785PurposeSunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selectionDepositorInsertSnoopCatcher-DNMT3A
Tags2xOLLASExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2 EGFP sgRNA2
Plasmid#164933PurposeExpresses EGFP sgRNA2 in mammalian cellsDepositorInsertsgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
UseLentiviralPromoterEFS promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAV1-K*R-HA
Plasmid#27765DepositorInsertcaveolin-1, lysine null mutant (CAV1 Human)
TagsHAExpressionMammalianMutationAll lysines mutated to arginines (K*R)Available SinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2 hSmad1-EVE-MM
Plasmid#22994DepositorInsertSmad1 (SMAD1 Human)
TagsFLAGExpressionMammalianMutationS214A, S206A, S195A, S187A, S463E, S465EAvailable SinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II KS(+) ACMV DNA-A 1.4mer
Plasmid#159134PurposeInfectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-ADepositorInsertACMV DNA-A 1.4mer
UseInfectious clone for bombarding plantsAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9
Plasmid#118842PurposeTargets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertcdkn2a gRNA (LOC100329528 Zebrafish)
UseCRISPR; Tol2Available SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbE1k-3
Plasmid#71722Purposetyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from humanDepositorPromoterpTrcAvailable SinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21ΔRING-Box
Plasmid#105523PurposeT7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryMutationdeletion of RING domain (aa124-462)PromoterT7Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBJ5 BRG1 DN
Plasmid#17874DepositorAvailable SinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
px330-CD4
Plasmid#136938PurposeNHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDHDepositorAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AktAR2-NES
Plasmid#175011PurposeCytosol-targeted AktAR2.DepositorInsertAktAR-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
eNme2-C TadDE
Plasmid#193842PurposeExpress TadDE (with eNme2-C variant) in mammalian cellsDepositorInserteNme2-C TadDE
ExpressionMammalianAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AktAR2-NLS
Plasmid#175012PurposeNuclear-targeted AktAR2.DepositorInsertAktAR-NLS
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB_Cdc42-TomKat-sensor-CAAX
Plasmid#172473PurposePiggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.DepositorInserttdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
UsePiggybac transposonTagspolybasic and CAAX motif from Kras, tdKatushka2, …ExpressionMammalianPromoterCAGAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
HR-TERT-promotor
Plasmid#71398PurposeHomologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.DepositorInsertTERT (TERT Human)
PromoterEndogenous TERT promotorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH
Plasmid#179586PurposeePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)DepositorInsertNLS-NG-T2A-hSHH
UsePiggybacTagsNeonGreenPromoterTREAvailable SinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human cofilin S3A
Plasmid#50854Purposeexpresses constituvely active cofilin in mammalian cellsDepositorInsertcofilin 1 S3A (CFL1 Human)
ExpressionMammalianMutationconstitutively active, S3APromoterCMVAvailable SinceFeb. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5/FRT/TO-FNTA-FLAG
Plasmid#86858PurposeTo express FNTA with a FLAG tag at C terminus.DepositorInsertFNTA (FNTA Human)
TagsFLAGExpressionMammalianMutationThe sequence of the insert is isoform 2, which di…PromoterCMVAvailable SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Actin-7
Plasmid#57339PurposeLocalization: Actin, Excitation: 505 / 569, Emission: 516 / 581DepositorAvailable SinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV_ER-RE_MLPmin_BC1065-luc2
Plasmid#227111PurposeEstrogen receptor response element for luciferase and barcode assays; barcode BC1065DepositorInsert12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304 GDF11-V5 blast
Plasmid#83098PurposeLentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tagDepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
3462 pSFFV-neo Bcl-2
Plasmid#8776DepositorInsertBcl-2 (Bcl2 Mouse)
ExpressionMammalianAvailable SinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSV-CaMKIV
Plasmid#45062PurposeExpression of CaMKIVDepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2+PhyBΔCreC
Plasmid#131781PurposeMammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.DepositorInsertPhyBCreC
UseCre/Lox; Zebrafish expressionExpressionMammalianPromoterCMVAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH3
Plasmid#168979PurposeAMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldADepositorTypeEmpty backboneUseSynthetic Biology; Heterologous expression in asp…Available SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAEL006511-Cas9
Plasmid#100580PurposeThe promoter of AAEL006511 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL006511-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL006511 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS
Plasmid#107319PurposeExpresses SpCas9 fused to dSaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to nuclease dead SaCas9 (D10A, N5…PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only