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We narrowed to 5,041 results for: U6...

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Showing: 4161 - 4180 of 5041 results

  3. PEA1-Nuclease-GFP

    Plasmid
    #171994
    Purpose
    Delivers all prime editing nuclease components in a single, GFP selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    171994-plasmid-map-sequence-id-338231

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  4. pCAT105

    Plasmid
    #180510
    Purpose
    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    DpbCasX with R3 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180510-plasmid-map-sequence-id-359734

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  6. pLKO_HA.NWS.VSVg_NGFR

    Plasmid
    #158243
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.NWS.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158243-plasmid-map-sequence-id-329479

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  8. pAcBac1-Mb-Fluoro-Phe D6

    Plasmid
    #197577
    Purpose
    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10
    Inserts
    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    Tags
    Nuclear export sequence + FLAG
    Expression
    Mammalian
    Mutation
    N311A, C313M, V366G, W382T
    Promoter
    CMV and U6/H1
    Available Since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197577-plasmid-map-sequence-id-388240

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  13. pLKO_HA.FLAG.VSVg_NGFR

    Plasmid
    #158245
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.FLAG.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158245-plasmid-map-sequence-id-327706

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  14. pLKO_NWS.FLAG.VSVg_NGFR

    Plasmid
    #158233
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.FLAG.VSVg
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158233-plasmid-map-sequence-id-327650

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  20. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain

    Plasmid
    #187276
    Purpose
    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domain
    Depositor
    Alpha Yap
    Article
    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.
    Insert
    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    Use
    Lentiviral
    Tags
    mCherry
    Mutation
    deltaActin binding domain
    Promoter
    U6, CMV
    Available Since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    187276-plasmid-map-sequence-id-372976

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Showing: 4161 - 4180 of 5041 results