181,839 results

1. pCIOX

   Plasmid

   #51300

   Purpose

   E.coli expression vector for His8-SUMO tagged recombinant protein

   Depositor

   Andrea Mattevi

   Type

   Empty backbone

   Use

   Tags

   His8 + SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSC-LHX8-IRES-GBX1

   Plasmid

   #182235

   Purpose

   To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with ASCL1, Sox11, FGF2 and two small molecules, forskolin and LDN-193189

   Depositor

   Chun-Li Zhang

   Article

   Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

   Insert

   LHX8-IRES-GBX1 (LHX8, GBX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCXLE-SOX2-p2a-KLF4

   Plasmid

   #193292

   Purpose

   episomal hSK (expressing human SOX2 + KLF4)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   SOX2-p2a-KLF4 (SOX2, KLF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F2A of original pCXLE-SK vector was replaced with…

   Promoter

   CAG

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CNR1-Tango

   Plasmid

   #66254

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   CNR1 (CNR1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. Shank2-HA /pCDNA3

   Plasmid

   #197334

   Purpose

   Plasmid expressing an antigen targeting Shank2

   Depositor

   Morgan Sheng

   Insert

   Shank2 (Shank2 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-PM-RasAR

   Plasmid

   #205569

   Purpose

   Plasma membrane-targeted RasAR.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   PM-RasAR (RAF1 Human, Synthetic)
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-5FLAG-hphMX6

   Plasmid

   #19342

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   hphMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-5FLAG-hphMX6

   Plasmid

   #19342

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   hphMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT010

   Plasmid

   #64762

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, HA to a 2xFLAG epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQCXIP-EGFP-F

    Plasmid

    #73014

    Purpose

    Retroviral expression of EGFP-F

    Depositor

    Michael Grusch

    Insert

    EGFP-F
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAR-Ec318

    Plasmid

    #130871

    Purpose

    A nuclear plasmid encoding the WT TP-DNAP1 for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. YFP NLS Beta-Actin S14C

    Plasmid

    #60614

    Purpose

    Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    S14C

    Promoter

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid

    #111903

    Purpose

    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.

    Depositor

    Andrew Carter

    Inserts

    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    

    Use

    Tags

    8xHis, SNAPf-tag, TEV site, and ZZ-tag

    Expression

    Insect

    Mutation

    Optimised for expression in Sf9 cells.

    Promoter

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Myc-CEBPA

    Plasmid

    #219393

    Purpose

    Express CEBPA in mammalian cells

    Depositor

    Yoichi Furukawa

    Article

    Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

    Insert

    CEBPA (CEBPA Human)
    

    Use

    Tags

    N terminal Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMT006

    Plasmid

    #64761

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, 2xFLAG to an HA-6xHIS epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. XLone-PE2

    Plasmid

    #136463

    Purpose

    Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Insert

    PE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFCiR2

    Plasmid

    #167512

    Purpose

    Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mRFP, Kanamycin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    CinRAM-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PCin

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pThermoCas9_ctrl

    Plasmid

    #100981

    Purpose

    Expresses ThermoCas9 and its sgRNA module

    Depositor

    John van der Oost

    Article

    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

    Inserts

    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    B. coagulans DSM 1 pta promoter and B. smithii xy…

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid

    #159908

    Purpose

    Mutagenesis of Htr2a

    Depositor

    Larry Zweifel

    Insert

    Htr2a (Htr2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRRL.PPT.SFFV.HA.RUNX1C.IRES.eGFP

    Plasmid

    #181980

    Purpose

    Human RUNX1C gene overexpression

    Depositor

    Jan-Henning Klusmann

    Insert

    RUNX1C (human) (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    HA (Human influenza hemagglutinin)

    Expression

    Mutation

    Promoter

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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