We narrowed to 9,280 results for: Pol;

1. TLCV2-sgHPDL #3

   Plasmid

   #174165

   Purpose

   knock out HPDL in human cell lines

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   sgRNA against HPDL
   

   Use

   Lentiviral

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TLCV2-sgHPDL #9

   Plasmid

   #174166

   Purpose

   knock out HPDL in human cell lines

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   sgRNA against HPDL
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCellFree_G03 CYP2D6

   Plasmid

   #67090

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CYP2D6 (CYP2D6 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD24-sfGFPx2

   Plasmid

   #51559

   Purpose

   Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

   Depositor

   Sankar Adhya, Francisco Malagon

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   superfolder GFP
   

   Tags

   GLESTCRHASLAVLADERRFSA and MARARA

   Expression

   Bacterial

   Mutation

   Contains additonal aa in the N and C terminus (in…

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEx-deletion-erm

   Plasmid

   #172213

   Purpose

   For allelic exchange in erythromycin-sensitive Prevotella copri strains

   Depositor

   Till Strowig

   Article

   Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

   Type

   Empty backbone

   Use

   Allelic exchange in prevotella copri

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AzoRS-4

   Plasmid

   #182884

   Purpose

   A pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

   Depositor

   Miriam Amiram

   Article

   Israeli et al Adv. Funct. Mater. 2021, 31, 2011276

   Insert

   AzoRS-4
   

   Expression

   Bacterial

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pK-myc-Par3b

   Plasmid

   #19388

   Depositor

   Ian Macara

   Article

   Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

   Insert

   Partitioning-defective protein 3b (PARD3 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Full length, isoform b.

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-CAG-GFPd2

   Plasmid

   #115665

   Purpose

   Piggybac transposon plasmid for destabilized eGFP (GFPd2)

   Depositor

   Jordan Green

   Article

   Rui et al ACS Appl Mater Interfaces. 2019 Feb 22.

   Insert

   GFPd2
   

   Use

   Synthetic Biology

   Tags

   Destabilization

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCellFree_G03 CYP3A4

   Plasmid

   #67104

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CYP3A4 (CYP3A4 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFW6000

    Plasmid

    #224215

    Purpose

    Bacteroides genomic editing tool; aTc inducible-Fncpf1

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 human cofilin WT

    Plasmid

    #50853

    Purpose

    expresses human cofilin in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 (CFL1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pmGFP-P2A-K(AAG)20-P2A-RFP

    Plasmid

    #105689

    Purpose

    Double fluorescent stalling reporter K(AAG)20

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Inserts

    P2A-3xFLAG-VHPbeta
    K(AAG)20
    P2A-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV (from vector)

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFPC1-6XHis-FLKSRP

    Plasmid

    #23001

    Depositor

    Douglas Black

    Article

    Hall et al Mol Biol Cell. 2004 Feb . 15(2):774-86.

    Insert

    KSRP (KHSRP Human)
    

    Tags

    EGFP and His

    Expression

    Mammalian

    Mutation

    valine inserted at aa#2

    Available Since

    Nov. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-mNFM

    Plasmid

    #32909

    Purpose

    Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of mouse neurofilament protein M

    Depositor

    Anthony Brown

    Article

    Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

    Insert

    EGFP-mouse NFM fusion (Nefm Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. HDM_RSV_A_1982_F

    Plasmid

    #237355

    Purpose

    Expression plasmid for RSV A F from 1982

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV A F from 1982 human codon optimized from Genbank Accesion KJ723472
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. HDM_RSV_A_2020_F

    Plasmid

    #237356

    Purpose

    Expression plasmid for RSV A F from 2020

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV A F from 2020 human codon optimized from Genbank Accesion PP495954
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA11-pMa-sgTS3-17xMS2V5-sgTS3

    Plasmid

    #199442

    Purpose

    CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    HITI donor template
    

    Use

    CRISPR

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. NylCK_pET28a

    Plasmid

    #215422

    Purpose

    Expression construct for NylCK gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCK
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS hShh (CT#401)

    Plasmid

    #13996

    Depositor

    Cliff Tabin

    Article

    Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

    Insert

    Shh (SHH Human)
    

    Expression

    Bacterial

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmGFP-P2A-K(AAA)12-P2A-RFP

    Plasmid

    #105687

    Purpose

    Double fluorescent stalling reporter K(AAA)12

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Inserts

    P2A-3xFLAG-VHPbeta
    K(AAA)12
    P2A-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV (from vector)

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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