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LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

Plasmid

#192405

Purpose

Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ

Plasmid

#192403

Purpose

Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

Plasmid

#192381

Purpose

To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

Plasmid

#192379

Purpose

To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

Plasmid

#192377

Purpose

To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO

Plasmid

#192378

Purpose

To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEY83

Plasmid

#191078

Purpose

flp-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-8 (flp-8 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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EK0544 CMV-TO-mCherry-SG(s70587-dblstop)SG-EGFP (FLP-IN)

Plasmid

#191176

Purpose

Mammalian expression of EK0209 with a second stop codon.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

CMV-TO:mCherry:s70587:EGFP (double-stop)

Expression

Mammalian

Mutation

additional stop codon

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0546 SFFV-mCherry-SGc(GAPDH.s1-dblstop)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191178

Purpose

Mammalian expression of EK0493 with second stop codon.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:GAPDH.s1:EGFP (double-stop)

Expression

Mammalian

Mutation

additional stop codon

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)

Plasmid

#191166

Purpose

Mammalian expression of a EGFP mutant N213D derived from EK0379.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

EGFP(N213D)

Expression

Mammalian

Mutation

c.637A>G(N213D; CCA>CCG)

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA

Plasmid

#191148

Purpose

AAV expression of a Cre reporter with EYFP output

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

DIO-EYFP

Use

AAV

Expression

Mammalian

Mutation

WT

Promoter

hEF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0362 SFFV-mCherry-SG(Bdnf.s1L36+54@202)SG-EGFP (FLP-IN)

Plasmid

#191153

Purpose

Mammalian expression of a 90 bp (36+54) long sensor for murine Bdnf 3' UTR where the trigger sequence is split

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1L36+54:EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_dPC_2xChromo

Plasmid

#179398

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

dPC_2xChromo

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-SpRYgest_targetplasmid (KAC833)

Plasmid

#181748

Purpose

pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Type

Empty backbone

Use

Substrate plasmid

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBI101.1/ProGOXL6:GUS

Plasmid

#175562

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL6

Tags

GUS

Expression

Plant

Promoter

GOXL6

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBI101.1/ProGOXL5:GUS

Plasmid

#175561

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL5

Tags

GUS

Expression

Plant

Promoter

GOXL5

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBI101.1/ProGOXL4:GUS

Plasmid

#175560

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL4

Tags

GUS

Expression

Plant

Promoter

GOXL4

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBI101.1/ProGOXL3:GUS

Plasmid

#175559

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL3

Tags

GUS

Expression

Plant

Promoter

GOXL3

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBI101.1/ProGOXL2:GUS

Plasmid

#175558

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL2

Tags

GUS

Expression

Plant

Promoter

GOXL2

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CaMKII-GCaMP6f-4x128-mAGNET

Plasmid

#166023

Purpose

Expresses GCaMP6f in CaMKII+ cells with low miRNA-128 expression

Depositor

Xue Han

Article

Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

Insert

GCaMP6f with 4 miR-128 binding sites

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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