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pC0078 RESCUE

Plasmid

#130661

Purpose

pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b-ADAR2dd (RESCUE)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLPCX mito roGFP2-Orp1

Plasmid

#64992

Purpose

Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Mutation

mitochondrial targeting sequence

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDV09_pCMV-ADAR1-p110

Plasmid

#200404

Purpose

Expresses ADAR1 (p110 isoform) constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

ADAR-p110

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE014

Plasmid

#108682

Purpose

All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)

Depositor

Takayuki Kohchi, Shigeo Sugano

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAT9624-BEAR-cloning

Plasmid

#162986

Purpose

BEAR target cloning plasmid with split EGFP

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

exchangeable cassette

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pM355

Plasmid

#173175

Purpose

(Empty Backbone) Expresses gRNA-12xMBS from hU6 promoter

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

gRNA-12xMBS backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PKA-SPARK

Plasmid

#106920

Purpose

Express PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

PKA-SPARK

Expression

Mammalian

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p)

Plasmid

#70210

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.nucGAP

Plasmid

#78736

Purpose

Expression of Ca2+ sensor GAP in the nucleus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

nucGAP

Tags

nucleoplasmin (Xenopus)

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pEdit

Plasmid

#232355

Purpose

The pEdit series plasmids are derived from the pTarget series plasmids by inserting homologous recombination arms targeting the gene of interest, which enables gene knockout or gene insertion at the t

Depositor

Yujie Cai

Article

Gou et al Biotechnol Bioeng. 2025 May;122(5):1233-

Insert

sgRNA

Use

CRISPR

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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3xmNeonGreen-3xrGBD

Plasmid

#176101

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pET21d-myc-BirA

Plasmid

#109424

Purpose

Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

BirA (birA )

Tags

myc

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ERK-SPARK

Plasmid

#106921

Purpose

Express ERK-SPARK, an activity reporter of ERK in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

ERK-SPARK

Expression

Mammalian

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBT374

Plasmid

#125615

Purpose

Expresses evoFERNY-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoFERNY-BE4max-NG

Expression

Mammalian

Mutation

H102P D104N

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pRDA_078

Plasmid

#158582

Purpose

Lentiviral expression of BE4max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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LeGO-Luc2

Plasmid

#154006

Purpose

Lentiviral vector expressing firefly luciferase (Luc2)

Depositor

Boris Fehse

Article

Ellinger et al Viruses. 2020 May 25;12(5). pii: v120505

Insert

Firefly Luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-Dvl2-GFP

Plasmid

#109148

Purpose

Stable Dvl2 expression

Depositor

Mariann Bienz

Article

Gammons et al J Cell Sci. 2016 Oct 15;129(20):3892-390

Insert

Dvl2 (DVL2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

SV40

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBT138b-proD

Plasmid

#138516

Purpose

P3e

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Frb-EGFP-HOTag6

Plasmid

#106919

Purpose

Express Frb-EGFP-HOTag6 fusion in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

Frb-EGFP-HOTag6

Expression

Mammalian

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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