Addgene

We narrowed to 29,586 results for: REP

Showing: 4321 - 4340 of 29586 results

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eGFP-KIF5A-Rigor

Plasmid

#172202

Purpose

Expresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

Insert

KIF5A (Kif5a Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Changed Glycine 235 to Alanine

Promoter

CMV

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78307

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADflluw

Use

Tags

Expression

Mutation

Promoter

Available since

July 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_C192A-Myc

Plasmid

#201168

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

C192A

Promoter

MESV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Ascl1.MIRT124_x4 Xma_lost.WPRE

Plasmid

#67290

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Asc1 (Ascl1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-PKA-RIIβ-sREACh

Plasmid

#114113

Purpose

Mammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

PKA regulatory subunit RII beta tagged by sREACh (low-irradiant YFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase

Plasmid

#188391

Purpose

Reporter for BCAM expression

Depositor

Markus Frank

Article

Sasamoto et al Cell Rep. 2022 Aug 9;40(6):111166. doi:

Insert

BCAM reporter

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221-TAZ CA

Plasmid

#79504

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WWTR1 (WWTR1 Human)

Use

Multisite gateway entry clone

Tags

3xFLAG

Expression

Mutation

S89A

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162336

Purpose

Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6; mU6

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-GCN4-dCEN-INCENP

Plasmid

#108510

Purpose

expression of vsv-GCN4-dCEN-INCENP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

GCN4-dCEN-INCENP (INCENP Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

replace CEN box for GCN4 dimerazation domain

Promoter

CMV

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGabrg1

Plasmid

#124856

Purpose

Mutagenesis of Gabrg1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg1 (Gabrg1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-UD

Plasmid

#140304

Purpose

Expresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

contains only the SH4 and UD regions

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-KD

Plasmid

#140296

Purpose

Expresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

contains only the SH4 and KD regions

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S32

Plasmid

#140308

Purpose

Expresses HA-tagged human Src-S32 in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

contains only the SH3 and SH2 domains

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

Plasmid

#124864

Purpose

Mutagenesis of Grin2b

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2b (Grin2b Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-JUN

Plasmid

#79512

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

JUN (JUN Human)

Use

Multisite gateway entry clone

Tags

HA Tag

Expression

Mutation

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pNIC28-BSA4-G-S18

Plasmid

#127825

Purpose

Production of recombinant human ribosomal protein S18

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS18 (RPS18 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGex4t2 with 244-458AA SUUR fragment

Plasmid

#112989

Purpose

Expresses fusion of GST and amino acids 244-458 of Drosophila SUUR

Depositor

Terry Orr-Weaver

Article

Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

Insert

SuUR (SuUR Fly)

Use

Tags

Expression

Bacterial

Mutation

amino acids 244-458

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAWp100

Plasmid

#157977

Purpose

pBT264-mutmU6p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgITGB1-1

Plasmid

#121533

Purpose

sgITGB1-1 sequence: GAAGCAGGGCCAAATTGTGGG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Stanley Qi, Ovijit Chaudhuri

Article

Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

Insert

sgITGB1-1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgITGB4-1

Plasmid

#121535

Purpose

sgITGB4-1 sequence: GAGGCGCAGTCCTTATCCACA. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Stanley Qi, Ovijit Chaudhuri

Article

Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

Insert

sgITGB4-1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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