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We narrowed to 18,346 results for: multi

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Showing: 4361 - 4380 of 18346 results

  2. pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

    Plasmid
    #175523
    Purpose
    Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells
    Depositor
    Kai Johnsson
    Publication
    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LAMP1-HaloTag11 and Tomm20-HaloTag9
    Expression
    Mammalian
    Mutation
    HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    175523-plasmid-map-sequence-id-343758

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  6. pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9

    Plasmid
    #84242
    Purpose
    Expresses GA-inducible KRAB-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9
    GID1-KRAB
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    84242-plasmid-map-sequence-id-160883

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  7. pBT272_(pRosa26-GTET)

    Plasmid
    #36882
    Depositor
    Liqun Luo
    Publication
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GFP
    tTA2
    beta-globin intron
    Neo
    tdT-3Myc
    insulator
    diphteria toxin A
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, inserti…
    Promoter
    CAG (chicken beta actin promoter and CMV enhancer…
    Available since
    Aug. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    36882-plasmid-map-sequence-id-46131

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  13. pBrain-GFP-TACC3KDP-shTACC3

    Plasmid
    #59356
    Purpose
    Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.
    Depositor
    Stephen Royle
    Publication
    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    Use
    RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Silent mutations in amino acids 30-33 to confer s…
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    59356-plasmid-map-sequence-id-94885

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  14. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid
    #132393
    Purpose
    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Publication
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Proline 301 to Leucine (P301L)
    Promoter
    TREtight
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    132393-plasmid-map-sequence-id-341619

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  15. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Publication
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

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  17. SP6-VEE-OKS-iG

    Plasmid
    #58975
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Publication
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58975-plasmid-map-sequence-id-91419

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  18. pQhsCDtRNA+

    Plasmid
    #86014
    Purpose
    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene
    Depositor
    Hong Li
    Publication
    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:
    Inserts
    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Promoter
    LPP and Ptac
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86014-plasmid-map-sequence-id-169485

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  19. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid
    #84244
    Purpose
    Expresses GA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sa dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    84244-plasmid-map-sequence-id-160887

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  20. pDN-D2irTNG4kwh

    Plasmid
    #44722
    Depositor
    Gabor Balazsi
    Publication
    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    pCMV-D2i promoter
    htetR::NLS::EGFP
    Use
    Synthetic Biology; Expression regulator/reporter;…
    Tags
    Rabbit β-globin intron II and WPRE
    Expression
    Mammalian
    Mutation
    Initiator motif (Inr) displaced relative to pCMV-…
    Promoter
    pCMV-D2i
    Available since
    April 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    44722-plasmid-map-sequence-id-61263

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Showing: 4361 - 4380 of 18346 results