Addgene

We narrowed to 1,608 results for: nif

Showing: 841 - 880 of 1608 results

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pKEW215_AcrIIA13

Plasmid

#138281

Purpose

Expression of AcrIIA13 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA13

Use

CRISPR

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPR

Plasmid

#113252

Purpose

pUC19 backbone with E. coli CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

E. coli CRISPR array

Use

Integration substrate

Tags

Expression

Mutation

Promoter

NA

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA5_gb36_lenti

Plasmid

#138306

Purpose

Lenti expression of AcrIIA5

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA5

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAW091

Plasmid

#113253

Purpose

pUC19 backbone with S. pyogenes CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes CRISPR array

Use

Integration substrate

Tags

Expression

Mutation

Promoter

NA

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p2CT-Cdi

Plasmid

#83044

Purpose

Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

Depositor

Jennifer Doudna

Article

Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

Insert

CdiCas9

Use

Tags

His-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only
pCF525-AcrIIA4_gb39_lenti

Plasmid

#138305

Purpose

Lenti expression of AcrIIA4

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA4

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac-Giardia Dicer

Plasmid

#89146

Purpose

Expression of Giardia Dicer in Sf9 cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

Giardia Dicer

Use

Tags

His6-teve

Expression

Insect

Mutation

Promoter

Available since

April 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA13b_gb76_GF31-2-hom_lenti

Plasmid

#138310

Purpose

Lenti expression of AcrIIA13b

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA13b

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA15_gb79_Cand27_lenti

Plasmid

#138313

Purpose

Lenti expression of AcrIIA15

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA15

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF525-AcrIIA14_gb77_Cand9_lenti

Plasmid

#138311

Purpose

Lenti expression of AcrIIA14

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKEW270-pTet-empty

Plasmid

#115669

Purpose

Vector for anti-CRISPR expression with pTet

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAIP4_4QMD

Plasmid

#234174

Purpose

Heterologous protein expression of envoplakin in Escherichia coli

Depositor

Christopher Bahl

Insert

4QMD (EVPL Human)

Use

Tags

10x HisTag-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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lentiGuideB3GNT5-neo

Plasmid

#220867

Purpose

encodes CRISPR sgRNA targeting human B3GNT5

Depositor

Jennifer Kohler

Insert

B3GNT5 (B3GNT5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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L0-T3 mTurq-tUBQ10

Plasmid

#195886

Purpose

Level 0 mTurq-tUBQ10 for integrase target construction

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

mTurq-UBQ10

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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L1P56

Plasmid

#195935

Purpose

Level 1 PhiC31 cassette with constitutive promoter : p35S:UbGln:PhiC31-NLS:tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

p35S:UbGln:PhiC31-NLS:tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P59

Plasmid

#195936

Purpose

Level 1 PhiC31-intein(DnaE-Npu) cassette : p35S-PI-t35S

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

p35S-PI-t35S

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P60

Plasmid

#195937

Purpose

Level 1 Intein(DnaE-Ssp)-PhiC31 cassette pUBQ10-IP-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10-IP-tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW092

Plasmid

#113250

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas1 H205A

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas1

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

H205A

Promoter

T7

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW188

Plasmid

#113178

Purpose

E. coli expression vector for untagged E. coli Cas1 R131A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R131A

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW181

Plasmid

#113127

Purpose

E. coli expression vector for His-MBP-E. coli Cas1 R138A

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Insert

Cas1

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R138A

Promoter

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW167

Plasmid

#113187

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 R40E

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R40E

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW156

Plasmid

#113185

Purpose

E. coli expression vector for untagged E. coli Cas1 K259E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

K259E

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW186

Plasmid

#113184

Purpose

E. coli expression vector for untagged E. coli Cas1 R163A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R163A

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW151

Plasmid

#113183

Purpose

E. coli expression vector for untagged E. coli Cas1 R146A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R146A

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW150

Plasmid

#113182

Purpose

E. coli expression vector for untagged E. coli Cas1 S143A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

S143A

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW174

Plasmid

#113181

Purpose

E. coli expression vector for untagged E. coli Cas1 R138A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R138A

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW189

Plasmid

#113180

Purpose

E. coli expression vector for untagged E. coli Cas1 Q136A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

Q136A

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW185

Plasmid

#113179

Purpose

E. coli expression vector for untagged E. coli Cas1 R132A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R132A

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW184

Plasmid

#113176

Purpose

E. coli expression vector for untagged E. coli Cas1 R112A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R112A

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW152

Plasmid

#113175

Purpose

E. coli expression vector for untagged E. coli Cas1 K12E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

K12E

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW158

Plasmid

#113177

Purpose

E. coli expression vector for untagged E. coli Cas1 R112E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Use

Tags

Expression

Bacterial

Mutation

R112E

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA707

Plasmid

#186238

Purpose

KanR-BBR1 entry vector for HR donor

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

Plasmid

#138315

Purpose

Lenti expression of mTgBFP2

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

mTAGBFP2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-TNFalpha

Plasmid

#28089

Depositor

Jennifer Stow

Article

Manderson et al J Cell Biol. 2007 Jul 2. 178(1):57-69.

Insert

TNFalpha (Tnf Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pKEW302-AcrVA4

Plasmid

#115656

Purpose

Expresses AcrVA4 in bacteria, under TetR control.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKEW303-AcrVA5

Plasmid

#115657

Purpose

Expresses AcrVA5 in bacteria, under TetR control.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
L1P80

Plasmid

#195941

Purpose

Level 1 pLBD16 reporter : pLBD16-mScarlet-NLS-tRBCS

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pLBD16-mScarlet-NLS-tRBCS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p2301y-tdtomato-dCas9_DegronDead_TPLN188

Plasmid

#205409

Purpose

dCas9 guided repressor

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

dCas9_DegronDead_TPLN188

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP8A-ccdB

Plasmid

#83883

Purpose

Destination vector for integration at HIS3, expression from the ADH1 promoter

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
L1_ARF19_4

Plasmid

#195916

Purpose

Level 1 PhiC31 cassette with LR dvp promoter: pARF19-PhiC31-DST-tUBQ1

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pARF19-PhiC31-DST-tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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