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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,976 results for: LEA

Showing: 11001 - 11050 of 15976 results

  1. DOT1L

    Plasmid
    #36196
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (GeneScript Synthesized) (DOT1L Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-420
    Available Since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. JMJD1CA-c089

    Plasmid
    #79644
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JMJD1C (JMJD1C Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79644-plasmid-map-sequence-id-150204

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  4. pMH220

    Plasmid
    #122836
    Purpose
    dCas9 with GreenGate B-C flanking sequences
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    Use
    Golden gate compatible cloning vector
    Mutation
    Contains D10A and H840A mutations
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122836-plasmid-map-sequence-id-239365

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  11. CBX4

    Plasmid
    #25237
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX4_02 (CBX4 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. MECR

    Plasmid
    #38850
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MECR (Mecr Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pGBW-m4046203

    Plasmid
    #145488
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145488-plasmid-map-sequence-id-306276

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  15. pQE30-TEV-pro-conA

    Plasmid
    #159527
    Purpose
    Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.
    Depositor
    Joshua Mylne
    Article
    Nonis et al bioRxiv. 2020.
    Insert
    Canavalia ensiformis pro-concanavalin A
    Tags
    Six-Histidine tag and tobacco etch virus (TEV) pr…
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    159527-plasmid-map-sequence-id-317076

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  21. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

    Plasmid
    #154399
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154399-plasmid-map-sequence-id-304590

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  34. PA-mCherry1-LaminA-C-18

    Plasmid
    #57185
    Purpose
    Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    LaminA (LMNA Human)
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pRSETA_crE6G2

    Plasmid
    #172929
    Purpose
    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain
    Depositor
    Arnold Boersma
    Article
    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.
    Insert
    CrowdingSensorE6G2
    Tags
    His
    Expression
    Bacterial
    Mutation
    Val317Gly, Met316Gly
    Promoter
    T7
    Available Since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172929-plasmid-map-sequence-id-339474

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  46. mEos3.2-LaminA-C-18

    Plasmid
    #57463
    Purpose
    Localization: Nuclear Envelope, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    LaminA (LMNA Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. pNUT3

    Plasmid
    #175744
    Purpose
    Single-component photo-inducible (CRY2-based) nucleolus-targeting tool 3
    Depositor
    Yubin Zhou
    Article
    Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655
    Insert
    mCherry-CRY2PHR-NoLS (human nuclear factor-κB inducing kinase)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    175744-plasmid-map-sequence-id-361253

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  48. CHD1 (CHD1A-c107)

    Plasmid
    #110253
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CHD1 (CHD1 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110253-plasmid-map-sequence-id-212335

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  49. pICSL50015

    Plasmid
    #50318
    Purpose
    Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)
    Depositor
    Nicola Patron
    Article
    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43
    Insert
    CDS, mNEON (Branchistoma lanceolatum)
    Use
    Plant expression
    Mutation
    BsaI/BbsI sites removed by point-mutation
    Available Since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    50318-plasmid-map-sequence-id-75075

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Showing: 11001 - 11050 of 15976 results