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We narrowed to 16,609 results for: IGH@

Showing: 11301 - 11350 of 16609 results
  1. pX330-Flag-dHeFSpCas9

    Plasmid
    #92116
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Insert
    dead/inactive HeFSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    Cbh
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pCMV_FLAG-NFL(K211TAG)

    Plasmid
    #182660
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position K211 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.
    Insert
    mouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    K211TAG
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. p1.1-Tr2-eGFP

    Plasmid
    #162782
    Purpose
    Fluorescent reporter for protein expression studies
    Depositor
    Insert
    enhanced green fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pICE-EGFP-FLAG-Ku70siR-Mut6E

    Plasmid
    #46962
    Purpose
    For constitutive or doxycycline-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Insert
    Ku70 (XRCC6 Human)
    Use
    Tags
    GFP-FLAG
    Expression
    Mammalian
    Mutation
    Mut6E corresponding to K282E K287E T300E K331E K3…
    Promoter
    CMV Tet
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
  5. pSN-A112C-GpA

    Plasmid
    #191238
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Use
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 11301 - 11350 of 16609 results