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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,855 results for: KIN

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Showing: 11351 - 11400 of 17855 results

  10. pVH028

    Plasmid
    #80393
    Purpose
    Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    Taz chimeric histine kinase
    CusS (cusS )
    NahR (nahR )
    Use
    Synthetic Biology
    Tags
    Fused by GS linkers to 4 SH3 domains
    Expression
    Bacterial
    Mutation
    Glycine 448 changed to Alanine, makes it primaril…
    Available Since
    Nov. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    80393-plasmid-map-sequence-id-166533

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  29. P47-COMP-blac-flag-his

    Plasmid
    #111000
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    secreted ookinete protein, putative (PSOP12) (PF3D7_0513700 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111000-plasmid-map-sequence-id-224544

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  30. SOAP-COMP-blac-flag-his

    Plasmid
    #110997
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    secreted ookinete adhesive protein (SOAP) (PF3D7_1404300 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110997-plasmid-map-sequence-id-224488

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  31. PF3D7_0620000-COMP-blac-flag-his

    Plasmid
    #110993
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    secreted ookinete protein 25, putative (PF3D7_0620000 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110993-plasmid-map-sequence-id-224484

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  32. P25-COMP-blac-flag-his

    Plasmid
    #110991
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    ookinete surface protein P25 (PF10_0303 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110991-plasmid-map-sequence-id-224481

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  34. A3A-Gam

    Plasmid
    #119769
    Purpose
    Conversion of C to T monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426
    Insert
    Gam-APOBEC3A-nCas9-NLS-2XUGI-NLS
    Tags
    NLS (flanking UGI)
    Expression
    Plant
    Mutation
    nCas9 has a mutation D10A in wild type SpCas9
    Promoter
    maize Ubiquitin-1
    Available Since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    119769-plasmid-map-sequence-id-257611

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  37. pREST-donor

    Plasmid
    #113807
    Purpose
    CRISPR donor plasmid to tag human transcription factor REST with GFP
    Depositor
    Kevin White
    Insert
    REST homology arms flanking EGFP-IRES-Neo cassette (REST Human)
    Use
    CRISPR
    Mutation
    rs4864608
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113807-plasmid-map-sequence-id-231037

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  39. AAV2_hSyn_iChloC_CA_Citrine

    Plasmid
    #98220
    Purpose
    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iChloC_C128A gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    E83Q, E90R, E101S, C128A, T159C, D156N
    Promoter
    human synapsin
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    98220-plasmid-map-sequence-id-256053

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  40. pDONR223_CASP8_WT_V5

    Plasmid
    #82999
    Purpose
    Gateway Donor vector containing CASP8, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    Jesse Boehm, Matthew Meyerson, David Root
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    CASP8 (CASP8 Human)
    Use
    Gateway Cloning
    Mutation
    AA 184_191delERSSSLEGinsGEELCGVMTISD; D194R; 196_…
    Promoter
    None
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82999-plasmid-map-sequence-id-162797

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  41. pFLAG-CMV2-Hsp27-S15D/S82D

    Plasmid
    #85186
    Purpose
    Hsp27 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp27 (HSPB1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S15D/S82D, phosphomimicking mutation
    Promoter
    CMV
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    85186-plasmid-map-sequence-id-167858

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  42. pFLAG-CMV2-Hsp27-S15D/S78D

    Plasmid
    #85185
    Purpose
    Hsp27 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp27 (HSPB1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S15D/S78D, phosphomimicking mutation
    Promoter
    CMV
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    85185-plasmid-map-sequence-id-167841

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  46. FLAG-KIF17-short-pcDNA3

    Plasmid
    #47545
    Purpose
    Expresses FLAG epitope-tagged short form of mouse KIF17 in mammalian cells
    Depositor
    Richard Maurer
    Insert
    KIF17-short (Kif17 Mouse)
    Tags
    FLAG epitope
    Expression
    Mammalian
    Mutation
    G152S*
    Promoter
    CMV
    Available Since
    Sept. 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    47545-plasmid-map-sequence-id-67853

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Showing: 11351 - 11400 of 17855 results