We narrowed to 7,981 results for: AMPH

1. pBzCas13b-gRNA-2

   Plasmid

   #164859

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   BzCas13b-gRNA-2
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBzCas13b-gRNA-3

   Plasmid

   #164860

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   BzCas13b-gRNA-3
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7gRNA-ccdb

   Plasmid

   #140864

   Purpose

   Backbone for making sgRNAs.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   ccdb/CAM
   

   Use

   Gateway destination vector

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDest-639 (attR4-attR2)

   Plasmid

   #161880

   Purpose

   Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDest-624 (attR4-attR2)

   Plasmid

   #161879

   Purpose

   Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEM-HypaCas9-D1135E

   Plasmid

   #154930

   Purpose

   aTc inducible HypaCas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   HypaCas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   N692A/M694A/Q695A/H698A/D1135E

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEM-Cas9HF1-D1135E

   Plasmid

   #154924

   Purpose

   aTc inducible Cas9HF1 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   Cas9HF1-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A/D1135E and stop codon on …

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPQD0CM0548

   Plasmid

   #162299

   Purpose

   Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   N-terminal tag, HiBiT-TrxA-[TEV cleavage site]
   

   Use

   Synthetic Biology

   Mutation

   codon optimized for E. coli

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEE22

   Plasmid

   #161787

   Purpose

   Hi-SC2 reporter delivery plasmid

   Depositor

   François-Xavier Barre

   Article

   Espinosa et al Mol Cell. 2020 Sep 3;79(5):857-869.e3. d

   Insert

   Hi-SC2 reporter
   

   Use

   Conjugative plasmid to deliver hi-sc2 reporter

   Mutation

   Himar site included MmeI site, tandem loxP sites,…

   Promoter

   n/a

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEPQD0CM0545

    Plasmid

    #162296

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, HiBiT-MBP-[Factor Xa cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPQD0CM0542

    Plasmid

    #162293

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, HiBiT-GST-[thrombin cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Integrase triple controller

    Plasmid

    #126540

    Purpose

    Pben-int5; Ptet-bxb1; Pbad-tp901: colE1 Chlo+

    Depositor

    Jerome Bonnet

    Article

    Zuniga et al Nat Commun. 2020 Sep 21;11(1):4758. doi:

    Insert

    BenR_pBEN-int5_pTet-bxb1_AraC_pBAD-tp901-1
    

    Expression

    Bacterial

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCellFree_KA1

    Plasmid

    #145369

    Purpose

    Gateway destination vector for cell-free and mamalian expression with N-term 8xHis and eGFP tag

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Type

    Empty backbone

    Tags

    His and eGFP

    Expression

    Bacterial

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACYCDuet1-ammA(∆50-56)

    Plasmid

    #134684

    Purpose

    Expresses His6-AmmA(∆50-56) in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    ammA(del50-56)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    deletion of amino acids 50-56

    Promoter

    T7

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACYCDuet1-ammA

    Plasmid

    #134682

    Purpose

    Expresses His6-AmmA in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    ammA
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR-P3P1r

    Plasmid

    #141014

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CSIV-DEST

    Plasmid

    #141053

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC-DEST-R1R4

    Plasmid

    #141054

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-DEST-R5R4-pHygTK

    Plasmid

    #141059

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    HygTK
    

    Expression

    Mammalian

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC-DEST-R5R4

    Plasmid

    #141055

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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