We narrowed to 81,643 results for: TRI

1. pGREAT1

   Plasmid

   #170889

   Purpose

   GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NOSt+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pNOS

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_mGFP-FGFR3

   Plasmid

   #191752

   Purpose

   Expression of N-terminally mGFP labelled wildtype FGFR3 (isoform IIIc)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Tags

   mGFP

   Expression

   Mammalian

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTEV5_ RpMatB_2

   Plasmid

   #78206

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

   Insert

   matB (His6 C106A S170A R286C Q457C K488A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A S170A R286C Q457C K488A

   Promoter

   T7

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3xFLAG-CMV14-mULK1

   Plasmid

   #24301

   Depositor

   Noboru Mizushima

   Article

   Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

   Insert

   a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pLenti-CMV-GFP-2A-G(N2c)-WPRE

   Plasmid

   #194352

   Purpose

   Lentivirus expressing eGFP and Rabies Glycoprotein (G) from CVS-N2c strain

   Depositor

   Marco Tripodi

   Article

   Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi

   Inserts

   Enhanced Green Fluorescent Protein (eGFP)
   Rabies CVS-N2c glycoprotein (G)
   

   Use

   Lentiviral

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-UGCG-KO

   Plasmid

   #80010

   Purpose

   gRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   UGCG (UGCG Human)
   

   Use

   CRISPR

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Nrxn1beta AS4(-)-Fc

   Plasmid

   #59313

   Purpose

   Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

   Depositor

   Peter Scheiffele, Tito Serafini

   Article

   Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

   Insert

   Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
   

   Tags

   Fc (from human IgG)

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-Promoter-4xBS_BHLHE40

   Plasmid

   #153064

   Purpose

   Four BHLHE40 consensus binding sites cloned upstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   4X consensus BHLHE40 binding sites
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. myc-BioID-Rab7

   Plasmid

   #121049

   Purpose

   Expresses myc tagged BioID and human Rab7a in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

   Inserts

   BioID
   Rab7A
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R118G (highly promiscuous form)

   Promoter

   CMV

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX Flag RagC WT

    Plasmid

    #112756

    Purpose

    express Flag-RagC

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p8103 LentiCRISPR v2 sgNT-2

    Plasmid

    #163313

    Purpose

    Expresses Cas9 and a non-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    sgNT-2
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-Akt(K179M)

    Plasmid

    #39535

    Depositor

    Julian Downward

    Article

    Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

    Insert

    Akt(K179M) (Akt1 Mouse)
    

    Tags

    6G linker, EGFP, and HA

    Expression

    Mammalian

    Mutation

    K179M

    Available Since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pMuLE_ENTR_TOP-NLuc1.1_L5-L4

    Plasmid

    #113867

    Purpose

    Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    TOP (wnt dependent promoter)
    NLuc
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIA1363

    Plasmid

    #166861

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp8 (ORF1ab Synthetic)
    

    Tags

    His-GB1

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-AU1-mTOR-L1460P

    Plasmid

    #19995

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    mammalian target of rapamycin-L1460P (Mtor Rat)
    

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    Changed Leucine 1460 to Proline

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-Nrxn1beta AS4(+)-Fc

    Plasmid

    #59314

    Purpose

    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

    Depositor

    Peter Scheiffele, Tito Serafini

    Article

    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

    Insert

    Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
    

    Tags

    Fc (from human IgG)

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRX-INU-TEX264 LIR4A-FLAG

    Plasmid

    #128259

    Purpose

    Expresses TEX264 LIR4A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    TEX264 LIR4A (TEX264 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed FEEL (aa 273-276) to alanines

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_nLightR

    Plasmid

    #217657

    Purpose

    Expresses norepinephrine indicator nLightR under mammalian promoter

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    nLightR
    

    Expression

    Mammalian

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIA1362

    Plasmid

    #166860

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp7 (ORF1ab Synthetic)
    

    Tags

    His-GB1

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

    Plasmid

    #182824

    Purpose

    Cre-dependent expression of cytosolic APEX

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    APEX.NES-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Promoter

    EF1a

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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