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Showing: 7141 - 7170 of 160936 results

  12. pD649-HAsp-IL11RA-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156788
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IL11RA (IL11RA Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156788-plasmid-map-sequence-id-426815

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  15. pANAP

    Plasmid
    #48696
    Purpose
    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS
    Depositor
    Peter Schultz
    Article
    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540
    Inserts
    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV and human H1 promoter
    Available since
    Nov. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    48696-plasmid-map-sequence-id-197837

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  19. pCFJ1320

    Plasmid
    #84829
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns
    Depositor
    Andrew Fire
    Article
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Use
    Tags
    Expression
    Bacterial and Worm
    Mutation
    Promoter
    Peft-3
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84829-plasmid-map-sequence-id-163669

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  21. mPlum-MyosinIIA-C-18

    Plasmid
    #55989
    Purpose
    Localization: Cytoskeleton, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    MyosinIIA (Myh9 Mouse)
    Use
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    55989-plasmid-map-sequence-id-209005

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  22. PRDM1

    Plasmid
    #25249
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PRDM1_04 (PRDM1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. pBBR15.2-2H12.D11opt-scarRef

    Plasmid
    #221150
    Purpose
    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2.1
    mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2.1)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221150-plasmid-map-sequence-id-437166

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Showing: 7141 - 7170 of 160936 results