259,103 results
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Plasmid#124494PurposeExpresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherryDepositorInsertFlipGFP(Caspase-1 cleavage seq) T2A mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR_HCV_xb
Plasmid#11510DepositorInsertFirefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#161928PurposeDonor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and Citrine activation reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpEFAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
SB100X in pCAG globin pA
Plasmid#127909PurposeSleeping Beauty Transposase expression vector driven by the CAG promoterDepositorInsertSleeping Beauty Transposase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) (AAV PHP.eB)
Viral Prep#199777-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) (#199777). In addition to the viral particles, you will also receive purified AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) plasmid DNA. Cre recombinase expression in striatal indirect pathway medium spiny neurons (D2-MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.PromoterTagsNoneAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Lifeact-7
Plasmid#54491PurposeLocalization: Action, Excitation: 587, Emission: 610DepositorTypeEmpty backboneUseTagsmCherry-LifeactExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
The Human Druggable CRISPR library
Pooled Library#200013PurposeThe library contains ~10,000 sgRNAs targeting 1,980 human genes (5 sgRNAs per gene and 100 nontargeting control sgRNAs).DepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_huCAD_ICADL (TevS)
Plasmid#100098Purposedual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequencesDepositorUseTagsExpressionBacterialMutationtwo caspase cleavage sites (DETD-117 and DAVD-224…PromoterT7Available sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PDi-CRISPRn
Plasmid#73500PurposeDox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locusDepositorInsertsCas9
rtTA
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCAG and TREAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
tetO-FOSL1
Plasmid#170687Purposedoxycycline-inducible overexpression of FOSL1DepositorInsertFOSL1 (FOSL1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-EGFP-CNA35
Plasmid#61603Purposebacterial expression of N-terminal fusion protein of CNA35 with EGFPDepositorInsertEGFP and CNA35
UseTagsHis tagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3 N-MYC TIP60
Plasmid#213003Purposeexpresses human TIP60DepositorInsertKAT5 (KAT5 Human)
UseTagsMYCExpressionMammalianMutationPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn WT
Plasmid#36046DepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
STICR_Index1
Pooled Library#186334PurposeHigh Complexity Lentiviral Barcode LibraryDepositorExpressionMammalianSpeciesSyntheticUseLentiviralAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_3XFlag
Plasmid#182494PurposeEmpty plasmid containing Flag tag for C-terminal taggingDepositorInsert3xFlag with 5 Glycolic spacer
UseTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-8
Plasmid#208786PurposeInducibly expresses human Caspase 8 fused to light-activatable Cry2DepositorInsertCaspase 8 (CASP8 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationCARD domain is removedPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
VPS13C^mClover3
Plasmid#118760PurposeVPS13C internally tagged with mClover3 at residue 1914.DepositorInsertVPS13C (VPS13C Human)
UseTagsmClover3 inserted at position aa 1914ExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti‐Cas9‐2A‐Blast
Plasmid#73310PurposeLentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoterDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS-NSAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Liu Mouse CRISPR Knockout Library in lentiCRISPRv2-blast
Pooled Library#1000000173DepositorAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K0-P2A-RFP
Plasmid#105686PurposeDouble fluorescent stalling reporter K0DepositorInsertsP2A-3xFLAG-VHPbeta
K0
P2A-mCherry
UseTagsExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2B-GFP
Plasmid#116869PurposeNuclear localized GFPDepositorInsertGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mCherry-IRES-Flpo (AAV Retrograde)
Viral Prep#55634-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-EF1a-mCherry-IRES-Flpo (#55634). In addition to the viral particles, you will also receive purified pAAV-EF1a-mCherry-IRES-Flpo plasmid DNA. Flpo and bicistronic (IRES) mCherry expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1a and Ef1a/IRESTagsmCherryAvailable sinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5B-Rigor
Plasmid#172204PurposeExpresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cellsDepositorInsertKIF5B (Kif5b Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-dCas9-KRAB-MeCP2
Plasmid#122267PurposeSP-dCas9-KRAB-MeCP2 with doxycycline-inducible expressionDepositorInsertdCas9-KRAB-MeCP2
UseTagsdCas9-KRAB-MeCP2ExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-mCherry (AAV8)
Viral Prep#137132-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-mCherry (#137132). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-mCherry plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre and Flp-dependent)Available sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flpo
Plasmid#60662PurposeExpresses pCAG-Flpo; codon-optimized for mammalian expressionDepositorInsertFlpo
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_25bpLinker
Plasmid#157788PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_25bp linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-scFvGCN4sfGFPTET1CD
Plasmid#82561PurposeExpression vector for antibody-sfGFP-TET1CD.DepositorInsertscFvGCN4sfGFPTET1CD
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only