We narrowed to 15,977 results for: LEA

1. pGBW-m4046829

   Plasmid

   #145637

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp11

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pGBW-m4046862

   Plasmid

   #145638

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF7b

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGBW-m4046912

   Plasmid

   #145639

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;Myc

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pGBW-m4046790

   Plasmid

   #145642

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp8

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pGBW-m4046827

   Plasmid

   #145625

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGBW-m4046960

   Plasmid

   #145628

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp11

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046749

   Plasmid

   #145629

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp11

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGBW-m4046903

   Plasmid

   #145632

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGBW-m4046823

   Plasmid

   #145634

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;Myc

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGBW-m4046917

    Plasmid

    #145620

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF7b

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pGBW-m4046984

    Plasmid

    #145621

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pGBW-m4046816

    Plasmid

    #145622

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGBW-m4046771

    Plasmid

    #145624

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGBW-m4046824

    Plasmid

    #145613

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGBW-m4046851

    Plasmid

    #145614

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046739

    Plasmid

    #145617

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4046737

    Plasmid

    #145605

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4046817

    Plasmid

    #145607

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046937

    Plasmid

    #145609

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046847

    Plasmid

    #145602

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF7b

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pGBW-m4046791

    Plasmid

    #145586

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pGBW-m4046805

    Plasmid

    #145589

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 endoRNAse

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pGBW-m4046935

    Plasmid

    #145580

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pGBW-m4046725

    Plasmid

    #145581

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp11

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pGBW-m4046761

    Plasmid

    #145582

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp11

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pGBW-m4046777

    Plasmid

    #145583

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pGBW-m4046734

    Plasmid

    #145576

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 endoRNAse

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pGBW-m4046826

    Plasmid

    #145687

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pME-TCAP

    Plasmid

    #109549

    Purpose

    Multisite gateway vector for 5' tagging with telethonin

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    telethonin (tcap Zebrafish)
    

    Use

    Zebrafish plasmids

    Mutation

    Please see Depositor Comments

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDN-D2irTNG5kwh

    Plasmid

    #128279

    Purpose

    Mammalian linearizer gene circuit in a Flp-In expression system with a hTetR::EGFP fusion protein

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    hTetR::NLS::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV-D2ir promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. ARB340

    Plasmid

    #124023

    Purpose

    Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear iRFP713

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    LS-A4_EF1a-NLS::iRFP713-bgPA_R1
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ARB341

    Plasmid

    #124024

    Purpose

    Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear tagBFP

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    LS-A4_EF1a-NLS::tagBFP-bgPA_R1
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MTK4a_009

    Plasmid

    #123956

    Purpose

    Encodes a nuclear localized EBFP2 (T2A) coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    T2A::NLS::EBFP2
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MTK4a_026

    Plasmid

    #123838

    Purpose

    Encodes ha-tagged and nuclear localized Abscisic Acid Insensitive as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    HA::2xNLS::ABI
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MTK4a_027

    Plasmid

    #123839

    Purpose

    Encodes ha-tagged and nuclear localized Gibberellin Insensitive as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    HA::2xNLS::GAI
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MTK4a_010

    Plasmid

    #123823

    Purpose

    Encodes a nuclear localized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    T2A::NLS::mRuby2
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MTK4a_011

    Plasmid

    #123824

    Purpose

    Encodes a nuclear localized iRFP670 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    T2A::NLS::iRFP670
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MTK4a_013

    Plasmid

    #123826

    Purpose

    Encodes a nuclear localized iRFP713 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    P2A::NLS::iRFP713
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MTK4a_016

    Plasmid

    #123829

    Purpose

    Encodes a nuclear localized tagBFP coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    P2a-NLS::tagBFP
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MTK4a_017

    Plasmid

    #123830

    Purpose

    Encodes a nuclear localized EBFP2 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    P2a-NLS::EBFP2
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. MTK4a_018

    Plasmid

    #123831

    Purpose

    Encodes a nuclear localized mTurquoise2 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    P2a-NLS::mTurquoise
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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42. MTK4a_021

    Plasmid

    #123834

    Purpose

    Encodes a nuclear localized and destabilized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    T2A::NLS::mRuby2::PEST4d
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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43. pHBDuet139

    Plasmid

    #121913

    Purpose

    Charge-Introduced NpuDnaB mini-intein

    Depositor

    Hideo Iwai

    Article

    Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

    Insert

    CI-NpuDnaBΔ290 intein
    

    Tags

    GB1 and His6-GB1

    Expression

    Bacterial

    Mutation

    Deletion of 290-residue endonuclease domain, I53K…

    Promoter

    T7

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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44. pHBDuet140

    Plasmid

    #121914

    Purpose

    Orthogonal NpuDnaB mini-intein intein

    Depositor

    Hideo Iwai

    Article

    Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

    Insert

    Oth-NpuDnaBΔ290 intein
    

    Tags

    GB1 and His6-GB1

    Expression

    Bacterial

    Mutation

    Deletion of 290-residue endonuclease domain, I53K…

    Promoter

    T7

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. pTH825-CEN-minRLuc/slowstaCFLuc

    Plasmid

    #115370

    Purpose

    Expresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferase

    Depositor

    Tobias von der Haar

    Inserts

    Gcn4 5'-UTR + Firefly Luciferase
    Renilla luciferase (codon minimised)
    

    Expression

    Yeast

    Mutation

    All codons have been changed for the least favour…

    Promoter

    ADH1 and TDH3

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. MtMscL delta49

    Plasmid

    #118342

    Purpose

    M. tuberculosis MscL (delta49) in pET19b

    Depositor

    Douglas Rees

    Article

    Herrera et al Sci Rep. 2018 Oct 1;8(1):14566. doi: 10.

    Insert

    MscL (mscL Mycobacterium tuberculosis)
    

    Tags

    6x His Tag and Thrombin Cleavage site

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 102 to Stop Codon

    Promoter

    T7 Promoter

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. LI F-G Gent

    Plasmid

    #104522

    Purpose

    LI resistance gene module. Please note that the plasmid contains Apramycin selection, not Gentamycin.

    Depositor

    Thomas Lahaye

    Article

    Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

    Insert

    Apramycin resistance gene
    

    Use

    Cloning vector

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pJOG822

    Plasmid

    #105430

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    YFP fused to SV40 Nuclear Localisation Signal
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. pJOG142

    Plasmid

    #105397

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    mYFP (GFP variant) fused to PKI nuclear export signal
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pJOG143

    Plasmid

    #105398

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    mYFP (GFP variant) fused to SV40 nuclear localization signal
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge