We narrowed to 19,554 results for: Tec

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pcDNA3.1 FLIPE-1m

Plasmid

#13544

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_TauFRET_3xKQ_wpre

Plasmid

#207522

Purpose

Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

Depositor

Bradley Hyman

Article

Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

Insert

TauFRET_P301L_3xKQ

Use

Lentiviral

Tags

NeonGreen and mTurquoise2

Mutation

P301L; K311Q; K317Q; K321Q

Promoter

EF1a

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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QPM-sgR (pTaU3)

Plasmid

#140448

Purpose

For sgRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

Insert

TaU3-sgRNA

Use

CRISPR

Expression

Plant

Promoter

TaU3

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pN7CAST

Plasmid

#190661

Purpose

Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB508-cTP-mCherry

Plasmid

#213862

Purpose

Binary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

Insert

Plastid transit peptide fused mCherry-UnaG

Expression

Bacterial and Plant

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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PrimeRoot.v2C-FLP

Plasmid

#201740

Purpose

For DNA insertion test in protoplast

Depositor

Caixia Gao

Article

Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

ePPE,FLP

Use

CRISPR

Expression

Plant

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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BPK3082

Plasmid

#78742

Purpose

Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

LbCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HaloTag N58-(GGS)X6-Lifeact

Plasmid

#171436

Purpose

Expression of HaloTag N58-(GGS)X6-Lifeact in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-Lifeact

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA

Plasmid

#194247

Purpose

Expresses 3 miRNAs targeting human alpha-Synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

miRaSynuclein(human) (SNCA Human)

Use

AAV and RNAi

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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GW1-mScarlet-Epac-GeNL

Plasmid

#207874

Purpose

Mammalian expression of the FRET/BRET cAMP sensor

Depositor

Mathew Tantama

Article

French et al ACS Omega. 2019 Sep 12;4(13):15504-15511

Insert

mScarlet-hEpac1(149-881)-mNeonGreen-NanoLuc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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TCARZ-CMV-Cherry-PAmin-GF3-R4A22

Plasmid

#203770

Purpose

For production of template RNA for use in PRINT, mCherry under CMV promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for mCherry

Use

Ivt template

Promoter

CMV

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pX330 spCas9-mSA

Plasmid

#113096

Purpose

pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

CAS9-MSA

Use

Synthetic Biology

Tags

MSA (monomeric streptavidin

Mutation

none

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAGC-Lb12-P1Bb

Plasmid

#231387

Purpose

For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis

Depositor

Qi-Jun Chen

Article

Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

Insert

U6-29

Use

CRISPR

Expression

Plant

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pT3-Neo-EF1a-GFP

Plasmid

#69134

Purpose

Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

Depositor

Martin Bonamino

Article

Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

Type

Empty backbone

Use

Sleeping beauty transposon

Expression

Mammalian

Promoter

U3MSCV

Available Since

Jan. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_ATP1.0

Plasmid

#167581

Purpose

Express the ATP sensor GRAB_ATP1.0 in Cre positive cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Promoter

EFS

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+cPLA2-mKate2

Plasmid

#162068

Purpose

Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

Depositor

Philipp Niethammer

Article

Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Avian, xenopus, zebrafish

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS1

Plasmid

#196977

Purpose

Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS1 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAF-HdrB3

Plasmid

#184908

Purpose

carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cas9-sgRNA-homology arms

Use

CRISPR; Broad-host-range

Expression

Bacterial

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA-SV40-puro

Plasmid

#240539

Purpose

Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBT29-splitD

Plasmid

#122599

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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