We narrowed to 26,694 results for: vit;

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pcDNA-h-mmPtch1-FL-TagBFP

Plasmid

#120921

Purpose

Expresses BFP tagged Ptch1

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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XE241 Rat frizzled-1-GFP-CS2P+

Plasmid

#16817

Depositor

Randall Moon

Article

Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

Insert

frizzled-1 (Fzd1 Rat)

Use

Xenopus expression

Tags

GFP

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pFastBac(His10-SNAP-Csk)

Plasmid

#177895

Purpose

Baculo expression of His10 tag fused to SNAPtag and human Csk

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Csk (CSK Human)

Tags

10xHis-TEV-TVMV-SNAP tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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RapR-Shp2-mVenus-flag

Plasmid

#188655

Purpose

Rapamycin regulated Shp2 phosphatase with mVenus and flag tag

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

Shp2

Tags

Flag and mVenus

Expression

Mammalian

Mutation

iFKBP insertion at V406 with deletion of residues…

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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645 pCS2-etv2

Plasmid

#49005

Purpose

Mammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Invitro transcription, riboprobe template

Expression

Mammalian

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM3-C71,72,105A

Plasmid

#58396

Purpose

Expressed murine IFITM3-C71,72,105A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteines 71, 72, and 105 to Alanine

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pME DN-E-cadherin

Plasmid

#135209

Purpose

Dominate Negative zebrafish e-cadherin label

Depositor

David Tobin

Article

Cronan et al Immunity. 2016 Oct 18;45(4):861-876. doi

Insert

CDH1

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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XE49 pt Beta-catenin-myc

Plasmid

#16840

Depositor

Randall Moon

Article

Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

Insert

pt beta-catenin-myc (ctnnb1.L Frog)

Use

Xenopus expression

Tags

6x myc

Mutation

four point mutations introduced into the GSK-3 ph…

Available Since

June 25, 2009

Availability

Academic Institutions and Nonprofits only
pegRNA_HEK4_20-7

Plasmid

#201974

Purpose

Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm

Depositor

Scot Wolfe

Article

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

pegRNA_HEK4_20-7

Expression

Mammalian

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A

Plasmid

#83483

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pGex-3x MKK4

Plasmid

#14617

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Tags

GST

Expression

Bacterial

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-hSyn-Gαq*-BERKY1

Plasmid

#158430

Purpose

BRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSyn

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-YFP-GRK2(RH)

Use

Lentiviral

Expression

Mammalian

Promoter

hSynapsin

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCASP-SptP120-3G124-HilA

Plasmid

#153323

Purpose

Triggers secretion of SptP120 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3G124 DARPin

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-1xSYN-EGFP

Plasmid

#19973

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

SYN-EGFP-WPRE (SYN1 jellyfish, Human)

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB rhoA WT

Plasmid

#61631

Purpose

mammalian expression of rhoA

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

rhoA (RHOA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pFKBPmCherry-Akt2(K14A)

Plasmid

#86597

Purpose

Expresses recruitable mCherry-tagged human Akt2(K14A) membrane-binding mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

FKBB-mCherry-Akt2(K14A) (AKT2 )

Expression

Mammalian

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSD117A

Plasmid

#218141

Purpose

Expresses AviTag-6xHis-MBP-TEV-SD40 for protein purification from E. coli

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD40

Tags

AviTag-6xHis-MBP-TEV-(GGGGS)2

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(K14A/R25E)

Plasmid

#86595

Purpose

Expresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E) (AKT2 )

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCB128

Plasmid

#205137

Purpose

Expression of mCherry-2xFYVE in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

2xFYVE (from human Hrs) (HGS Synthetic)

Use

Dictyostelium expression

Tags

mCherry

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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CP1028-ABE8e-dimer

Plasmid

#138498

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PsopB-GFPLVA

Plasmid

#23341

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pILGFP97

Plasmid

#83551

Purpose

Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

CYC1 promoter-yEGFP

Expression

Yeast

Promoter

CYC1

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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FUp95GW (B7)

Plasmid

#74016

Purpose

lentiviral expression of shRNA resistant Psd95-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 (DLG4 Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pTYF-sGFAP-LOx-IRES-tdTomato

Plasmid

#166919

Purpose

Express the bacterial lactate oxidase (LOx) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Insert

Lactate oxidase

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-TSC2-NLS

Plasmid

#192441

Purpose

Encodes mCherry-tagged wild-type TSC2 isoform 5 targeted to the nucleus.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2-NLS (TSC2 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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p-mCherry-N1-iChloC

Plasmid

#85467

Purpose

improved slowChloC artificial anion conducting ChR. Codon optimized for mammalian expression (human/mouse)

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

Insert

iChloC

Tags

mCherry

Expression

Mammalian

Mutation

E83Q, E90R, E101S, T159C, D156N

Promoter

CMV (+enhancer)

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-evoA1-seBEC

Plasmid

#174702

Purpose

Small-molecule controlled base editing

Depositor

Rahul Kohli

Article

Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

Insert

Lenti-evoA1-seBEC

Use

Lentiviral

Tags

flag tag

Mutation

UGI-E11K

Promoter

EFS

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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RS18960_pMQ30_KO_Kan

Plasmid

#185393

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS18960 upstream flanking homology region
RR42_RS18960 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgGFP-2

Plasmid

#74961

Purpose

Cas9 + sgGFP-2 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

GFP

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pILL5-NLS-3xmCitrine

Plasmid

#192525

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

IAA-leu-resistant-like5 (ILL5 Mustard Weed)

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

ILL5

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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