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We narrowed to 44,295 results for: tra

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Showing: 4901 - 4920 of 44295 results

  1. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196048-plasmid-map-sequence-id-385883

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  4. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

    Plasmid
    #49077
    Purpose
    Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x HA and mCerulean
    Expression
    Mammalian
    Mutation
    262 silent mutations from native hNKCC1
    Promoter
    CMV
    Available Since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49077-plasmid-map-sequence-id-72205

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  7. pPFO*

    Plasmid
    #120400
    Purpose
    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.
    Depositor
    Arun Radhakrishnan
    Article
    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11
    Insert
    Mutant form of Perfringolysin O
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Amino acids 1-27 are deleted, starts at amino aci…
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120400-plasmid-map-sequence-id-233255

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  20. pHRE

    Plasmid
    #134909
    Purpose
    Overexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.
    Depositor
    George Lomonossoff
    Article
    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    134909-plasmid-map-sequence-id-267026

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Showing: 4901 - 4920 of 44295 results