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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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173,387 results

Showing: 9881 - 9920 of 173387 results

  1. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-498727

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  10. mPlum-VE-Cadherin-N-10

    Plasmid
    #56005
    Purpose
    Localization: Tight Junctions, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    VE-Cadherin (CDH5 Human)
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    I503T
    Promoter
    CMV
    Available Since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    56005-plasmid-map-sequence-id-474989

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  11. Perox-B

    Plasmid
    #209867
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP B
    Tags
    Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209867-plasmid-map-sequence-id-415401

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  17. TFORF2053

    Plasmid
    #142912
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BCL6 (BCL6 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142912-plasmid-map-sequence-id-391564

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  25. ZJOM136

    Plasmid
    #133660
    Purpose
    Integration of ELO3-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-ELO3-mTagBFP2 (ELO3 Budding Yeast)
    Tags
    mTagBFP2
    Expression
    Yeast
    Available Since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133660-plasmid-map-sequence-id-265888

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  29. pExp-Trx

    Plasmid
    #112577
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-TrxA and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112577-plasmid-map-sequence-id-217212

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Showing: 9881 - 9920 of 173387 results