We narrowed to 5,419 results for: U6 gRNA

1. pmU6-gRNA

   Plasmid

   #53187

   Purpose

   Expresses the S. pyogenes sgRNA from the mouse U6 promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

   Plasmid

   #164266

   Purpose

   sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6 promoter from W05B2.8

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)

   Plasmid

   #216871

   Purpose

   This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

   Depositor

   Ori Bar-Nur

   Article

   Lenardic et al J Clin Invest. 2024 May 7:e166998. doi:

   Insert

   Cas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   mdx mutation in mouse dystrophin

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFD3.1-w-dU6:3gRNA

   Plasmid

   #123366

   Purpose

   gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

   Depositor

   Simon Bullock

   Insert

   U6:3-gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA-eGFP-Reporter

   Plasmid

   #60719

   Purpose

   Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and mouse U6

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_ATP1A1_G7_Dual_sgRNA

   Plasmid

   #173206

   Purpose

   Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_ATP1A1_G3_Dual_sgRNA

   Plasmid

   #173205

   Purpose

   Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

   Plasmid

   #184381

   Purpose

   This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

   Depositor

   Ori Bar-Nur

   Article

   Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

   Insert

   Cas9, two gRNAs targeting dystrophin
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-Cj-sgRNA

   Plasmid

   #89753

   Purpose

   U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   Cj-SgRNA
   

   Use

   Sgrna expression under u6 promoter

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-gRNA

    Plasmid

    #53062

    Purpose

    Transcription of guide RNA (gRNA) in wheat cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    wheat U6 promoter - gRNA
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    wheat U6

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFD5-U6-3-t-attP40

    Plasmid

    #133561

    Purpose

    gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    attP40 region guide RNAs
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9_sgRNA_0

    Plasmid

    #70763

    Purpose

    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating

    Depositor

    Regine Kahmann

    Article

    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10

    Inserts

    U6 promoter
    cas9
    tnos terminator
    

    Use

    Self-replicating in ustilago maydis, conferring c…

    Tags

    N-terminal NLS, C-terminal HA-tag +NLS

    Expression

    Mutation

    Streptococcus pyogenes gene codon-optimized for U…

    Promoter

    U6 from Ustilago maydis and synthetic otef promot…

    Available since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-U6-shR-Scr

    Plasmid

    #19800

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Insert

    scrambled shRNA
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

14. p-mCherry2-sgRNA (empty)

    Plasmid

    #198330

    Purpose

    Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    U6-sgRNA(F+E) empty
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRII-Topo U6-SpAi9L-U6SpDMDL

    Plasmid

    #78608

    Purpose

    Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9L-U6SpDMDL
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

16. pX330-U6-E690_guide-CBh-hSpCas9

    Plasmid

    #188545

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-U6-W308_guide-CBh-hSpCas9

    Plasmid

    #188546

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-U6-L101_guide-CBh-hSpCas9

    Plasmid

    #188547

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. U6-TM2Emp-6T_iBlue

    Plasmid

    #200235

    Purpose

    Backbone for cloning short RNA triggers that activate iSBH-sgRNAs

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PlayBack-U6-Xba1

    Plasmid

    #203300

    Purpose

    For expression of sgRNAs in a PlayBack Vector with XbaI as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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