167,513 results

1. pTW2159

   Plasmid

   #236582

   Purpose

   ISYmu1 CHLl1 gRNA9 TRV2 Architecture_B

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Insert

   ISYmu1 TnpB
   

   Expression

   Plant

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTW2155

   Plasmid

   #236579

   Purpose

   ISYmu1 CHLl1 gRNA5 TRV2 Architecture_B

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Insert

   ISYmu1 TnpB
   

   Expression

   Plant

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. FIRESFLIP-PBCS-E670

   Plasmid

   #228701

   Purpose

   Sensor cassette FIRE2, sensor in backwards orientation, FIRE fluorescent ratiometric sensor

   Depositor

   Carolyn Sangokoya

   Article

   Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

   Insert

   FIRE2 SENSOR
   

   Use

   Synthetic Biology and Unspecified

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a(+)-T7-OmpA-MNaseB-6xHis

   Plasmid

   #214809

   Purpose

   Expression of 6xhis-tagged micrococcal nuclease B (MNaseB)

   Depositor

   Peter Sarin

   Article

   Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

   Insert

   OmpA-Micrococcal nuclease B
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNLF1-C_CSNK2A2:1-350

   Plasmid

   #211081

   Purpose

   Mammalian expression of full-length human CSNK2A2. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   CSNK2A2:M1-R350 (CSNK2A2 Human)
   

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

   Plasmid

   #213728

   Purpose

   Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

   Depositor

   Chaolin Zhang

   Article

   Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

   Insert

   bIV spectrin (repeats 13-15) (Sptbn4 Mouse)
   

   Expression

   Mammalian

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-SlugCas9-P2A-puro

   Plasmid

   #214353

   Purpose

   Expresses SlugCas9 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCas9 and puromycin resistance genes
   

   Expression

   Mammalian

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pfa6a mScarlet-FKBP::KANMX

   Plasmid

   #185905

   Purpose

   Plasmid for cloning mScarlet fused to FKBP into yeast.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   mScarlet-FKBP
   

   Tags

   mScarlet fused to FKBP

   Expression

   Yeast

   Promoter

   SP6

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH47811-pAtAct2::Cas9-nls::tSlATPase

   Plasmid

   #160221

   Purpose

   Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   pAtACT2::Cas9-nls::tSlATPase
   

   Use

   Synthetic Biology; Moclo toolkit level 1 acceptor…

   Expression

   Plant

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPR-tagBFP

    Plasmid

    #75160

    Purpose

    Derived from LentiCRISPR v1 but expresses tagBFP instead of puromycin resistance

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV2/7

    Plasmid

    #112863

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap7
    

    Use

    AAV

    Promoter

    Truncated P5

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid

    #112021

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta/alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Sept. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-mBaoJin-P2A-FusionRed

    Plasmid

    #229572

    Purpose

    Expresses mBaoJin and FusionRed in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    mBaoJin
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

    Plasmid

    #214102

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Expression

    Mammalian

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Myc-NLRP3(R260W)

    Plasmid

    #73956

    Purpose

    Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    R260W

    Promoter

    CMV iE

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

16. GFP-JIP3

    Plasmid

    #164624

    Purpose

    Expresses JIP3 with a GFP tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    JIP3 (MAPK8IP3 Human)
    

    Tags

    cycle 3 GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLR5-CAG-44B12mut23(Ser5ph)-SNAP

    Plasmid

    #174877

    Purpose

    PiggyBac transposon vector for expression of RNAPII Ser5ph mintbody

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    RNAPII Ser5ph mintbody-SNAP
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pON.mCherry

    Plasmid

    #84821

    Purpose

    Constitutive Expression plasmid with mCherry fluorescent protein

    Depositor

    Howard Shuman

    Article

    Gebhardt et al PLoS One. 2017 Mar 3;12(3):e0173116. doi

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    Tac Promoter

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. MCP-M-MLV RT∆RNase H

    Plasmid

    #213749

    Purpose

    For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    MCP-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST14-SpyCatcher

    Plasmid

    #35044

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyCatcher
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Deletion of C-terminal till I113, and point mutat…

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1-MEF2C-HA

    Plasmid

    #32515

    Depositor

    Andrew Lassar

    Article

    Kozhemyakina et al Mol Cell Biol. 2009 Nov;29(21):5751-62.

    Insert

    Mef2c (Mef2c Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEM040 [Multiome Perturb-seq sgRNA expression vector]

    Plasmid

    #224898

    Purpose

    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments

    Depositor

    Thomas Norman

    Article

    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.

    Inserts

    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBAMD1-2

    Plasmid

    #61564

    Purpose

    Standardized mini-Tn5 delivery plasmid, kanamycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Available Since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. ABE-NW-NL

    Plasmid

    #240621

    Purpose

    Adenine base editor with narrowed editing window

    Depositor

    Tingting Jiang

    Article

    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

    Insert

    TadA8e(N108Q,M151C,Q154Y)-nCas9 (D10A)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    TadA8e(N108Q,M151C,Q154Y), Cas9(D10A)

    Available Since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEGFP-C1 Lifeact-EGFP

    Plasmid

    #58470

    Purpose

    Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Bioarchitecture. 2015 Aug 28:1-14.

    Insert

    Lifeact
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. PIK3CA - Exon20 H1047R

    Plasmid

    #16639

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H1047R

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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27. Su9-EGFP

    Plasmid

    #23214

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Subunit 9 of the F0-ATPase
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains AA 1-69 of subunit 9

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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28. pANAP

    Plasmid

    #48696

    Purpose

    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

    Depositor

    Peter Schultz

    Article

    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

    Inserts

    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    

    Expression

    Mammalian

    Mutation

    mutant E.coli leucyl synthetase specific for Anap…

    Promoter

    CMV and human H1 promoter

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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29. pSQT1601

    Plasmid

    #53369

    Purpose

    human codon optimized Csy4 and FokI-dCas9 expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    hCsy4-T2A-NLS-hFokI-dCas9-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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30. pICH-roGFP2-ORP1

    Plasmid

    #191677

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    redox(ro)GFP2-ORP1
    

    Use

    Binary agrobacterium ti plasmid

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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