167,514 results

1. Ki67-mNeonGreen-IRESpuro2

   Plasmid

   #183737

   Purpose

   Expresses Ki67-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 (MKI67 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11b-MIF

   Plasmid

   #149446

   Purpose

   Expression of WT MIF in E. coli for protein production

   Depositor

   Elias Lolis

   Insert

   MIF (MIF Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS415-GPD

   Plasmid

   #183940

   Purpose

   Control vector that has a GPD promoter with no insert

   Depositor

   Michael Downey

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. T7-p73DD-pcDNA3

   Plasmid

   #22978

   Depositor

   William Kaelin

   Article

   Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

   Insert

   tumor protein p73 (TP73 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Mutation

   truncated cDNA encodes truncated protein (aa 327-…

   Available Since

   Jan. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 TEV (full-length)

   Plasmid

   #64276

   Purpose

   Expresses tobacco etch virus protease (TEV)

   Depositor

   Xiaokun Shu

   Article

   To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Insert

   Tobacco etch virus (TEV) protease
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cx36-GCaMP

   Plasmid

   #123604

   Purpose

   Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

   Depositor

   John O'Brien

   Article

   Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

   Insert

   Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
   

   Tags

   GCaMP3

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PCMV-intron myc Rab2 S20N

   Plasmid

   #46780

   Purpose

   expression of S20N Rab2a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB2 (RAB2A Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S20N dominant negative

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pYZ463

   Plasmid

   #187971

   Purpose

   TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBDC

   Plasmid

   #135188

   Purpose

   Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   CmR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Promoter

   cat

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. YES1 gRNA (BRDN0001148961)

    Plasmid

    #77967

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human YES1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    YES1 (YES1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. YES1 gRNA (BRDN0001145317)

    Plasmid

    #77965

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human YES1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    YES1 (YES1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. YES1 gRNA (BRDN0001487120)

    Plasmid

    #77966

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human YES1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    YES1 (YES1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICE-HA-NLS-I-PpoI

    Plasmid

    #46963

    Purpose

    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    NLS-I-PpoI
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV-TET

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLD-puro-MTS-EGFP

    Plasmid

    #190270

    Purpose

    Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

    Depositor

    Christian Münch

    Article

    Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

    Insert

    MTS-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCap-101-Hgy

    Plasmid

    #227508

    Purpose

    Capture plasmid with hygromycin resistance and pIJ101 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209725

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-NLS-HA-Dre

    Plasmid

    #51272

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Dre
    

    Tags

    NLS-HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA WDR24 pRK5

    Plasmid

    #46335

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR24 (WDR24 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pN3-MGA

    Plasmid

    #107715

    Purpose

    Expression plasmid for 3xFLAG-tagged MGA

    Depositor

    Guntram Suske

    Article

    Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

    Insert

    MGA (MGA Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pET28a-6xHis-MBP-TEV site-TadA8.20

    Plasmid

    #194702

    Purpose

    Expresses N-terminal MBP fused TadA8.20 in bacteria

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    TadA8.20
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    T7 promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. evoBE4-SpRY

    Plasmid

    #226854

    Purpose

    Expresses evoBE4max-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    evoBE4max-SpRY
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. qTAG-C-V5-Puro

    Plasmid

    #207730

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. pLM1110_LEU2

    Plasmid

    #168460

    Purpose

    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    mRFP1
    

    Use

    Synthetic Biology; Yeast assembly vector for big-…

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. PB533-44B12m23-sfGFP

    Plasmid

    #186778

    Purpose

    RNAP2 Ser5ph-mintbody expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    44B12m23-scFv
    

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. IGF2BP1

    Plasmid

    #155676

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    IGF2BP1 (IGF2BP1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRel-Hyg

    Plasmid

    #227582

    Purpose

    Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-hSyn1-eYFP (AAV5)

    Viral Prep

    #117382-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Promoter

    Syn

    Tags

    eYFP

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

28. pCDZ2FE_Pogz_1-1410

    Plasmid

    #105674

    Purpose

    Expresses Flag-tagged full-length human POGZ protein

    Depositor

    Thanos Halazonetis

    Insert

    POGZ (POGZ Human)
    

    Tags

    2X Flag tag

    Expression

    Mammalian

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pAAV-Syn-DIO-NaChBac-dTomato

    Plasmid

    #236252

    Purpose

    Cre dependent NaChBac-TdTomato expression

    Depositor

    Janelia Viral Tools

    Article

    Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

    Insert

    NaChBac-TdTomato
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. M43: pHR-SFFVp-iLID::EGFP::FTH1

    Plasmid

    #122149

    Purpose

    Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Lentiviral

    Tags

    NLS-iLID-GFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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