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pESC-MET

Plasmid

#222482

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and MET15 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-LYS

Plasmid

#222483

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Trip-GFP-hOCLN

Plasmid

#32789

Depositor

Tianyi Wang

Article

Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

Insert

OCLN (OCLN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only
pRL25

Plasmid

#70253

Purpose

Shuttle vector suitable for transfer to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains

Depositor

Peter Wolk

Article

Wolk et al J Bacteriol. 1988 Mar;170(3):1239-44.

Type

Empty backbone

Use

Bacterial shuttle vector

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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HS-Par3-GFP

Plasmid

#105947

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Par3

Tags

GFP

Expression

Bacterial

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-HisYFP-Sp100

Plasmid

#53141

Purpose

E.coli expression of HisYFP tagged human Sp100 fragment

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

Sp100A fragment (SP100 Human)

Tags

His-YFP

Expression

Bacterial

Mutation

fragment (amino acids 71-480); deletion of L75

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pRK7-Rheb

Plasmid

#15888

Depositor

John Blenis

Article

Tee et al Curr Biol. 2003 Aug 5. 13(15):1259-68.

Insert

Rheb (RHEB Human)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pAGT9403

Plasmid

#192352

Purpose

L0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) with stop

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

TTCG_Stop-tNOS_CGCT

Expression

Plant

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-ECL-3TM-tevs-KKMP V3

Plasmid

#179640

Purpose

TEVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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TC358

Plasmid

#122613

Purpose

TRE-delta-pes-10-GFP

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

7XTRE-delta-pes-10-GFP

Expression

Worm

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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RFP-eNOS-NLS

Plasmid

#22496

Depositor

William Sessa

Article

Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

Insert

eNOS(G2A)-NLS

Tags

RFP

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pGL2-Full

Plasmid

#16012

Depositor

Wafik El-Deiry

Article

Takimoto et al Oncogene. 2000 Mar 30. 19(14):1735-43.

Insert

KILLER/DR5 (TNFRSF10B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-) FLIPQTV3.0 8m

Plasmid

#63732

Purpose

FLIPQTV3.0 glutamine sensor with R75MY86A mutations

Depositor

Sakiko Okumoto

Article

Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

Insert

FLIPQTV3.0 sensor, 8mM affinity

Expression

Mammalian

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-MKRN1

Plasmid

#78745

Purpose

To overexpress MKRN1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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HA-BICD2(1-500)-FRB

Plasmid

#174648

Purpose

Rapalog-inducible coupling to dynein adaptor BICD2

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

HA-BICD2(1-500)-FRB

Tags

FRB and HA

Expression

Mammalian

Mutation

mmBICD2(aa1-500): Lys213Glu

Promoter

Chicken beta-actin

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBad-Xis/Int Reset Flipper

Plasmid

#38207

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

Bxb1-Excisionase
Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Promoter

pBAD

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-LacZshRNA

Plasmid

#73978

Purpose

LacZshRNA (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the LacZ gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-NL1A

Plasmid

#15227

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 with insert A (Nlgn1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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TR-Csy4

Plasmid

#80601

Purpose

Expresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4

Use

AAV

Expression

Mammalian

Promoter

CBA

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAG1242 NLS-mCherry-FIREtag

Plasmid

#205170

Purpose

Expresses NLS-mCherry-FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

NLS-mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET-11-LECT2-V40

Plasmid

#183031

Purpose

Expression of LECT2 V40 variant protein from T7 promoter

Depositor

Gareth Morgan

Article

Belonogov et al bioRxiv 2022.03.01.482540

Insert

LECT2 (LECT2 Human)

Expression

Bacterial

Mutation

V40 variant

Promoter

T7

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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EcpH-TM

Plasmid

#85389

Purpose

Expresses ecliptic pHluorin–ICAM-1 (intercellular adhesion molecule 1) in mammalian cells. Incorporates into viral membrane to label pseudoviruses

Depositor

Gregory Melikyan

Article

Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do

Insert

ecliptic pHluorin (EcpH) fused to N-terminus of human ICAM-1 transmembrane domain

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T2-Rheb

Plasmid

#15889

Depositor

John Blenis

Article

Tee et al Curr Biol. 2003 Aug 5. 13(15):1259-68.

Insert

Rheb (RHEB Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pWN172

Plasmid

#215291

Purpose

Expresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Tags

His

Expression

Bacterial

Promoter

J23113

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCBFRE-(mt)-luc

Plasmid

#26896

Depositor

Nicholas Gaiano

Article

Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

Insert

CBFRE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated CBF1 sites (see note)

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pBad-Int Set Flipper

Plasmid

#38209

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Insert

Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Tags

His

Promoter

pBAD/AraC

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pMN-296

Plasmid

#195454

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF3 BS - ybTATA - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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mscv2.2-NAIP1

Plasmid

#60200

Purpose

Mouse NAIP1 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NAIP1

Depositor

Russell Vance

Article

Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

Insert

NAIP1

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-MYT1L

Plasmid

#32926

Purpose

Retroviral expression of human Myt1L

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

MYT1L (MYT1L Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pGY208

Plasmid

#239803

Purpose

Express mCherry in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

mCherry

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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p8.9 NdeltaSB D185K/D186L

Plasmid

#132931

Purpose

p8.9 NdeltaSB bearing RT-D185K/D186L mutant reverse transcriptase sequence

Depositor

Jeremy Luban

Article

De Iaco et al Retrovirology. 2011 Dec 6;8:98. doi: 10.

Insert

gag and pol D185K/D186L

Use

Lentiviral

Mutation

D185K/D186L

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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PKC beta II DN

Plasmid

#16385

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K371R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAG1238 PINK1-mCherry-FIREtag

Plasmid

#205174

Purpose

Expresses PINK1-mCherry-FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

PINK1-mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-SauCas9

Plasmid

#174040

Purpose

In vitro transcription of SauCas9 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

SauCas9

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Endoplasmic reticulum-targeted optoPLD

Plasmid

#140060

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-Sac1(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CIBN, CRY2, P2A, Sac1(TM), and mCherry

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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aav-tnt-gfp-v2

Plasmid

#165036

Purpose

AAV-based delivery of GFP into cardiomyocytes in vivo

Depositor

William Pu

Article

Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Type

Empty backbone

Use

AAV

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF.111

Plasmid

#227187

Purpose

pORTMAGE-Ec1 + supplement E. coli SSB

Depositor

Seth Shipman

Article

Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

Insert

CspRecT, mutL_E32K, E. coli SSB

Expression

Bacterial

Mutation

mutL E32K

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mscv2.2-NAIP2

Plasmid

#60201

Purpose

Mouse NAIP2 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NAIP2

Depositor

Russell Vance

Article

Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

Insert

NAIP2 (Naip2 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMZ-180

Plasmid

#195428

Purpose

Transient, constitutive expression of synZiFTR: ZF5-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF5-7 (ZF5) - p65 - RbGpA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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mSA-phi29

Plasmid

#210815

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

mSA-Phi29

Expression

Bacterial

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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