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We narrowed to 2,417 results for: CAG promoter

Showing: 1251 - 1300 of 2417 results

  7. pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

    Plasmid
    #197952
    Purpose
    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pcoCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU6-26 and pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197952-plasmid-map-sequence-id-387870

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  11. pC1300_pUB10_pcoCASphi_E9t_V2_2x35Sp_HSP18t_ribozyme_AtPDS3_gRNA10

    Plasmid
    #197959
    Purpose
    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by 2x35Sp and flanked by ribozymes.
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pcoCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S promoter and pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197959-plasmid-map-sequence-id-387883

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  12. pC1300_pUB10_pcoCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

    Plasmid
    #197960
    Purpose
    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by UBQ10 gene promoter and flanked by ribozymes.
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pcoCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197960-plasmid-map-sequence-id-388015

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  13. ATP1A1_G8_MTOR_Nick_Intron-45_Dual_sgRNA

    Plasmid
    #178106
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 + MTOR nick intron-45 sgRNAs
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178106-plasmid-map-sequence-id-350189

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  14. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

    Plasmid
    #68405
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
    FlpO
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68405-plasmid-map-sequence-id-119955

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  15. pSP571

    Plasmid
    #139498
    Purpose
    CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA
    Depositor
    Serge Pelet
    Article
    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:
    Insert
    pGPD Cas9 / sgRNA (STL162)
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    pGPD
    Available since
    June 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139498-plasmid-map-sequence-id-275585

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  16. CMV:AsCpf1-2A-GFP-U6-GFP-sg

    Plasmid
    #194717
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target GFP
    Depositor
    Rudolf Jaenisch
    Article
    Haiting et al bioRxiv
    Insert
    AsCpf1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    194717-plasmid-map-sequence-id-381222

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  18. pNSU299_RRT8

    Plasmid
    #166080
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RRT8 (RRT8 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166080-plasmid-map-sequence-id-330356

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  19. MtPT4-p3

    Plasmid
    #160003
    Purpose
    Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Inserts
    DsRed (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160003-plasmid-map-sequence-id-323050

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  23. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid
    #160139
    Purpose
    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    Use
    In vitro transcription of sgrna from t7 promoter
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160139-plasmid-map-sequence-id-333510

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  26. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201151
    Purpose
    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    SadCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mutation
    gRNA sequence: ATCAGTGATAGAGAACGTATG
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201151-plasmid-map-sequence-id-401792

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  27. pNSU299_RPL39

    Plasmid
    #166078
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166078-plasmid-map-sequence-id-330349

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  28. pL0-MtBCP1Pro

    Plasmid
    #159415
    Purpose
    Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Insert
    MtBCP1 Promoter
    Use
    Puc19-derived
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    159415-plasmid-map-sequence-id-323165

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  29. pCKC764

    Plasmid
    #226625
    Purpose
    piggyBAC pCAG-hCas9-T2A-Unbiased3
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT S. pyogenes, Human, Chicken)
    EF1a promoter, blasticidin resistance
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226625-plasmid-map-sequence-id-449160

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  30. pAAV-shMcu-1

    Plasmid
    #181868
    Purpose
    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter
    Depositor
    Hilmar Bading
    Article
    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc
    Insert
    mitochondrial calcium uniporter (Mcu Mouse)
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181868-plasmid-map-sequence-id-356032

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  32. pAAV-shMcu-2

    Plasmid
    #181867
    Purpose
    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter
    Depositor
    Hilmar Bading
    Article
    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc
    Insert
    mitochondrial calcium uniporter (Mcu Mouse)
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181867-plasmid-map-sequence-id-356033

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  37. Banshee-ShCit-A

    Plasmid
    #85216
    Purpose
    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.
    Depositor
    Alex Minella
    Article
    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002
    Insert
    shCit-A
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1
    Available since
    Feb. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    85216-plasmid-map-sequence-id-171277

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  38. Banshee-ShCit-B

    Plasmid
    #85217
    Purpose
    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.
    Depositor
    Alex Minella
    Article
    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002
    Insert
    shCit-B
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1
    Available since
    Feb. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    85217-plasmid-map-sequence-id-171275

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  39. pmIL-6 mut AP-1+C/EBP

    Plasmid
    #61292
    Purpose
    drives luciferase from mouse IL-6 promoter with mutations in both AP-1 & C/EBP sites
    Depositor
    Gail Bishop
    Article
    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Mutated both AP-1 binding site from TGAGTCT to TG…
    Promoter
    IL-6 promoter with mutant AP-1 and C/EBP binding …
    Available since
    May 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  40. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid
    #68356
    Purpose
    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68356-plasmid-map-sequence-id-119628

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  41. CBSH3- 3m shRNA3

    Plasmid
    #160213
    Purpose
    Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Tags
    Expression
    Mutation
    TGTATGACCTCaGGcTGtACCA (mutations shown in lower …
    Promoter
    mouse U6 promoter
    Available since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    160213-plasmid-map-sequence-id-314924

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  42. CBSH3- 3m shRNA2

    Plasmid
    #160211
    Purpose
    Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Tags
    Expression
    Mutation
    TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …
    Promoter
    mouse U6 promoter
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160211-plasmid-map-sequence-id-313962

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  43. CBSH3- 3m shRNA1

    Plasmid
    #160209
    Purpose
    Knock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Tags
    Expression
    Mutation
    GATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…
    Promoter
    mouse U6 promoter
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160209-plasmid-map-sequence-id-313960

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  44. pX551-miniCMV-SpCas9D10A nickase

    Plasmid
    #216735
    Purpose
    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    T7 and mini-CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216735-plasmid-map-sequence-id-430321

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  45. pAAV-nEF-SpCas9D10A nickase

    Plasmid
    #216736
    Purpose
    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    EF-1-alpha core
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216736-plasmid-map-sequence-id-430323

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Showing: 1251 - 1300 of 2417 results