168,956 results

1. pcDNA3.1(-).TagRFP.T-2A-V5-Tau2N4R-WPRE

   Plasmid

   #226381

   Purpose

   Expresses tau propagation ORF with V5 tag on WT 2N4R tau

   Depositor

   Bradley Hyman

   Article

   Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

   Insert

   Tau2N4R (MAPT synthetic, Human)
   

   Tags

   TagRFP.T.2A.V5

   Expression

   Mammalian

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TfR-pHuji

   Plasmid

   #61505

   Purpose

   Human transferrin receptor tagged with pH sensitive red fluorescent protein pHuji

   Depositor

   David Perrais

   Article

   Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

   Insert

   Transferrin receptor (TFRC Human)
   

   Tags

   pHuji

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJYS3_ΔcrtYf

   Plasmid

   #85542

   Purpose

   Constitutive transcription of FnCpf1 and crRNA of crtYf

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   crRNA of crtYf
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Expression

   Bacterial

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.3-enCjCas9-BlastR

   Plasmid

   #220978

   Purpose

   Expresses human codon-optimized enCjCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   enCjCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. vHB8

   Plasmid

   #193454

   Purpose

   Expression of GFP-Cas9-NLS and gRNA in A. castellanii

   Depositor

   Chantal Abergel

   Article

   Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

   Insert

   GFP-Cas9-NLS
   

   Use

   CRISPR

   Tags

   GFP

   Promoter

   GAPDH

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn1-eYFP (AAV9)

   Viral Prep

   #117382-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Promoter

   Syn

   Tags

   eYFP

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

7. pET28sumo-hRig-I-Full-length

   Plasmid

   #210827

   Purpose

   protein expression in ecoli

   Depositor

   Anna Pyle

   Article

   Luo et al Structure. 2012 Nov 7;20(11):1983-8. doi

   Insert

   hRIG-I (DDX58) Full length protein (RIGI Human)
   

   Expression

   Bacterial

   Mutation

   NA

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-beta-catenin deltaN90

   Plasmid

   #36985

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   beta-catenin deltaN90 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 1-90 (Constitutively Acti…

   Promoter

   EF1a

   Available Since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPR

   Plasmid

   #42875

   Purpose

   A crRNA expression plasmid for targeting a specific sequence.

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   CRISPR-BsaI
   

   Use

   CRISPR; E.coli

   Expression

   Bacterial

   Available Since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

    Plasmid

    #134858

    Purpose

    Expresses EGFP-tagged early endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Ras-related protein 5A
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+ Grx1-roCherry

    Plasmid

    #141068

    Purpose

    Fluorescent biosensor for 2GSH/GSSG ratio

    Depositor

    Dmitry Bilan

    Article

    Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

    Insert

    Grx1-roCherry
    

    Expression

    Mammalian

    Mutation

    Changed Alanine 150 to Cysteine, inserted Threon…

    Promoter

    CMV, SP6

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid

    #185913

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLJM1-FIRE-pHLy

    Plasmid

    #170775

    Purpose

    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    CMV

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR221-NR1H2

    Plasmid

    #79513

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    LXR BETA (NR1H2 Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-CRX-T2A-NeuroD1

    Plasmid

    #194607

    Purpose

    Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

    Depositor

    Haruhisa Inoue

    Article

    Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

    Insert

    CRX-T2A-NeuroD1
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. v5 Cas9-eVLP

    Plasmid

    #228468

    Purpose

    Expresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag(C507V)–Cas9
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    MMLVgag(C507V)

    Promoter

    CMV

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-FOXO3a WT

    Plasmid

    #1787

    Depositor

    Michael Greenberg

    Article

    Brunet et al Cell 1999 Mar 19;96(6):857-68.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP_mNG2(11)_Clathrin light chain

    Plasmid

    #82608

    Purpose

    Expresses mNG2(11) tagged clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNG2(11)_Clathrin light chain
    

    Expression

    Mammalian

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLminP_Luc2P_RE17

    Plasmid

    #90359

    Purpose

    NFAT - PKC/Ca++ gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    NFAT

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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