163,387 results

1. pcDNA3-CXCL12-sfGFP

   Plasmid

   #98961

   Purpose

   Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CXCL12 (CXCL12 Human)
   

   Use

   Tags

   Superfolder GFP

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 His-V5 Myc

   Plasmid

   #176049

   Purpose

   Expresses Myc tagged with His and V5 in Mammalian cells

   Depositor

   Linda Penn

   Article

   Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

   Insert

   Myc
   

   Use

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1-GfaABC1D-Rpl22-HA

   Plasmid

   #111811

   Purpose

   Express Rpl22HA in astrocytes

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Ribosomal protein L22 (Rpl22 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-RFP

   Plasmid

   #22907

   Depositor

   Edward Callaway

   Article

   Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

   Insert

   human Synapsin I promoter driving DsRedExpress (SYN1 Human)
   

   Use

   AAV; Adeno-associated virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_SETDB1_WT

   Plasmid

   #82291

   Purpose

   Gateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SETDB1 (SETDB1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60228

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Centrin2-N-10

   Plasmid

   #55018

   Purpose

   Localization: Centrosomes, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Centrin2 (CETN2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAuroraA-GFP-AURKA-mCherry

   Plasmid

   #99878

   Purpose

   Expression of GFP-AURKA-mCherry in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   AURKA minimal

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mPiezo2-CMV-Sport6

   Plasmid

   #81073

   Purpose

   mouse Piezo2

   Depositor

   Ardem Patapoutian

   Article

   Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

   Insert

   Piezo2 (please see depositor comments below) (Piezo2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

    Plasmid

    #141378

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pHAGE-BRAF-V600E

    Plasmid

    #116204

    Purpose

    Lentiviral expression of BRAF V600E

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    V600E

    Promoter

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221899

    Purpose

    Expresses alpha tubulin fused to mStayGold

    Depositor

    Janelia Integrative Imaging

    Insert

    mStayGold-aTubulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG148 FRB-NFAST

    Plasmid

    #130812

    Purpose

    Expresses FRB-NFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

    Insert

    FRB-NFAST
    

    Use

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBHRSF184

    Plasmid

    #89482

    Purpose

    Express asparaginyl endopeptidase 1 from O. affinis (OaAEP1)

    Depositor

    Hideo Iwai

    Article

    Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

    Insert

    Asparaginyl endopeptidase from Oldenlandia affinis
    

    Use

    Tags

    Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETSXM2-pLacI-mtrCAB

    Plasmid

    #174618

    Purpose

    Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    mtrCAB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-mCherry-Rheb

    Plasmid

    #192436

    Purpose

    Encodes mCherry-tagged wild-type Rheb.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-Rheb (RHEB Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid

    #90085

    Purpose

    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA

    Depositor

    Raffaella Sordella

    Article

    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Destabilized Domain and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS3 (CK2alpha, CK2beta)

    Plasmid

    #27092

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Inserts

    CK2beta (CSNK2B Human)
    CK2alpha (CSNK2A1 Human)
    

    Use

    Tetracycline-regulated expression

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    bidirectional tet-responsive promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. DsRed-rab7 WT

    Plasmid

    #12661

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV CAMKII PSAM4 GlyR IRES EGFP (AAV5)

    Viral Prep

    #119744-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from AAV CAMKII PSAM4 GlyR IRES EGFP (#119744). In addition to the viral particles, you will also receive purified AAV CAMKII PSAM4 GlyR IRES EGFP plasmid DNA. CamKII-driven expression of PSAM4 GlyR IRES EGFP for neuronal silencing. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Promoter

    CaMKII

    Tags

    EGFP

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

21. PCR4-Shh::lacZ-H11

    Plasmid

    #139098

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Shh

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pWXC078

    Plasmid

    #222961

    Purpose

    C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

    Depositor

    Oliver Hobert

    Article

    Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

    Insert

    mScarlet-I3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAV-5S-Squash

    Plasmid

    #177914

    Purpose

    Expresses 5S rRNA fused to Squash aptamer in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Squash aptamer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5S

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mCherry-Alpha-Actin-12

    Plasmid

    #54971

    Purpose

    Localization: Actin, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Alpha-Actin (ACTA2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA

    Plasmid

    #171383

    Purpose

    Expression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized minidCas13X.1-REPAIRv2
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1(+)-iBB-GECO1

    Plasmid

    #194215

    Purpose

    Far-red fluorescent Ca2+ ion indicator for mammalian cell expression.

    Depositor

    Jin Zhang

    Article

    Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

    Insert

    iBB-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

    Plasmid

    #141373

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pCMV-eNme2-C-ABE8e

    Plasmid

    #185667

    Purpose

    Expresses eNme2-C-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. VN-Tau (wt)

    Plasmid

    #87368

    Purpose

    expresses hTau fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-TAF1

    Plasmid

    #65395

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    TAF1 (TAF1 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge