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pU6-sgRNA EF1Alpha-puro-T2A-BFP

Plasmid

#60955

Purpose

Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP

Plasmid

#60955

Purpose

Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-rDA3h

Plasmid

#208703

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3h

Use

AAV

Promoter

hSyn

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-rDA3h

Plasmid

#208703

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3h

Use

AAV

Promoter

hSyn

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTransposon.CAG.CasRX-P2A-Blast

Plasmid

#212965

Purpose

Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.

Depositor

Roland Rad

Article

Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

Insert

CasRx

Use

Transposon

Tags

P2A-Blasticidin resistance

Expression

Mammalian

Promoter

CAGGS

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTransposon.CAG.CasRX-P2A-Blast

Plasmid

#212965

Purpose

Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.

Depositor

Roland Rad

Article

Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

Insert

CasRx

Use

Transposon

Tags

P2A-Blasticidin resistance

Expression

Mammalian

Promoter

CAGGS

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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5HT6-mCherry-G-GECO1.0

Plasmid

#47500

Purpose

Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

G-GECO1.0

Tags

5HT6 and mCherry

Expression

Mammalian

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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5HT6-mCherry-G-GECO1.0

Plasmid

#47500

Purpose

Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

G-GECO1.0

Tags

5HT6 and mCherry

Expression

Mammalian

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pGEMS-II-PEST

Plasmid

#204766

Purpose

GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

EGFP-PEST-m6Alinker-DHFR

Mutation

mutated all RACs in EGFP to RGC/RCC

Promoter

CMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMS-II-PEST

Plasmid

#204766

Purpose

GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

EGFP-PEST-m6Alinker-DHFR

Mutation

mutated all RACs in EGFP to RGC/RCC

Promoter

CMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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GoldenBraid 2.0 Kit

Plasmid Kit

#1000000076

Purpose

Modular cloning of multigenic DNA constructs in Plant Synthetic Biology applications. 95 GoldenBraid cloning compatible plasmids, containing standard basic parts and transcriptional units

Depositor

Diego Orzaez

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

Plasmid

#182287

Purpose

Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Inserts

codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT

Tags

nuclear export signal (NES)

Expression

Mammalian

Mutation

Y306A/Y384F (AF) double mutant of Methanosarcina …

Promoter

CMV and U6

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

Plasmid

#182287

Purpose

Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Inserts

codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT

Tags

nuclear export signal (NES)

Expression

Mammalian

Mutation

Y306A/Y384F (AF) double mutant of Methanosarcina …

Promoter

CMV and U6

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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LifeAct-HaloTag

Plasmid

#176105

Purpose

F-actin marker

Depositor

Dorus Gadella

Insert

LifeAct

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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LifeAct-HaloTag

Plasmid

#176105

Purpose

F-actin marker

Depositor

Dorus Gadella

Insert

LifeAct

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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3HA-ERHBD-Hoxb8

Plasmid

#212932

Purpose

Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

Depositor

Hans Haecker

Article

Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

Insert

3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Mouse, Human)

Use

Retroviral

Tags

Triple HA tag

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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3HA-ERHBD-Hoxb8

Plasmid

#212932

Purpose

Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

Depositor

Hans Haecker

Article

Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

Insert

3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Mouse, Human)

Use

Retroviral

Tags

Triple HA tag

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Prig-3::CD4::spGFP1-10

Plasmid

#65829

Purpose

For membrane contact

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)

Tags

spGFP1-10

Expression

Worm

Promoter

Prig-3

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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Prig-3::CD4::spGFP1-10

Plasmid

#65829

Purpose

For membrane contact

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)

Tags

spGFP1-10

Expression

Worm

Promoter

Prig-3

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

Plasmid

#61762

Purpose

Dual Color betaGlobin reporter to study splicing kinetics in vivo

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

b-Globin

Tags

24 X MS2 hairpin cassette in 3'UTR of b-Glob…

Expression

Mammalian

Promoter

Dox inducible Tet-ON promoter

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

Plasmid

#61762

Purpose

Dual Color betaGlobin reporter to study splicing kinetics in vivo

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

b-Globin

Tags

24 X MS2 hairpin cassette in 3'UTR of b-Glob…

Expression

Mammalian

Promoter

Dox inducible Tet-ON promoter

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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DLX2-hygro

Plasmid

#97330

Purpose

Generating lentiviruses to express Dlx2 and hygromycin resistance

Depositor

Marius Wernig

Article

Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

Insert

DLX2 (Dlx2 Mouse)

Use

Lentiviral

Promoter

TetO

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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DLX2-hygro

Plasmid

#97330

Purpose

Generating lentiviruses to express Dlx2 and hygromycin resistance

Depositor

Marius Wernig

Article

Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

Insert

DLX2 (Dlx2 Mouse)

Use

Lentiviral

Promoter

TetO

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3

Plasmid

#75127

Purpose

Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3

Plasmid

#75127

Purpose

Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-Myoferlin HA

Plasmid

#22443

Depositor

William Sessa

Article

Bernatchez et al J Biol Chem. 2007 Oct 19. 282(42):30745-

Insert

Myoferlin

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-Myoferlin HA

Plasmid

#22443

Depositor

William Sessa

Article

Bernatchez et al J Biol Chem. 2007 Oct 19. 282(42):30745-

Insert

Myoferlin

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-puro GFP-p62

Plasmid

#38277

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-puro GFP-p62

Plasmid

#38277

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pINDUCER dCas9-TET1CD

Plasmid

#101921

Purpose

The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

dCas9-TET1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge