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We narrowed to 25,285 results for: crispr

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Showing: 5261 - 5280 of 25285 results

  2. pDHt/sk-CE

    Plasmid
    #92128
    Purpose
    Expression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reesei
    Depositor
    Zhihua Zhou
    Publication
    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-eGFP
    Use
    CRISPR; Trichoderma reesei expression
    Tags
    EGFP
    Promoter
    Pcbh2
    Available since
    Nov. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92128-plasmid-map-sequence-id-195044

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  4. GuideVector_BbsI

    Plasmid
    #110692
    Purpose
    For rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.
    Depositor
    Douglas Mahoney
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110692-plasmid-map-sequence-id-211319

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  6. GG-EBNA-OSK2M2L1-PP

    Plasmid
    #102902
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OSK2M2L1-gRNAs-PGK-puro
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    102902-plasmid-map-sequence-id-198388

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  8. pY036_ATP1A1_G3_Array

    Plasmid
    #86619
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    86619-plasmid-map-sequence-id-168855

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  10. pYL-kasOp-FnCas12a2

    Plasmid
    #177874
    Purpose
    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.
    Depositor
    Yunzi Luo
    Publication
    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptomyces codon optimized FnCas12a
    Use
    CRISPR
    Promoter
    kasOp*
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    177874-plasmid-map-sequence-id-352015

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  12. pRha-ABE8e-NRCH

    Plasmid
    #165417
    Purpose
    For bacterial purification of ABE8e-NRCH protein
    Depositor
    David Liu
    Insert
    ABE8e-NRCH
    Tags
    8xHIS and BP-NLS
    Expression
    Bacterial
    Promoter
    pRhaBAD
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165417-plasmid-map-sequence-id-334014

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  14. PB-tight-DDdCas9GFP-IRES-Neo

    Plasmid
    #102907
    Purpose
    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102907-plasmid-map-sequence-id-198394

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  16. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    104910-plasmid-map-sequence-id-206061

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  17. pXPR_016

    Plasmid
    #107143
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    David Root
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    107143-plasmid-map-sequence-id-205293

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  18. dCas9-dMSK1

    Plasmid
    #165602
    Purpose
    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)
    Depositor
    Isaac Hilton
    Publication
    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1
    Insert
    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Synthetic, S. Pyogenes, Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    165602-plasmid-map-sequence-id-327024

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  19. P628_SpCas9-RecA

    Plasmid
    #87263
    Purpose
    Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency
    Depositor
    Yonglun Luo
    Publication
    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RecA
    Use
    CRISPR
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    no
    Promoter
    CBh
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    87263-plasmid-map-sequence-id-173851

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Showing: 5261 - 5280 of 25285 results