We narrowed to 17,434 results for: She

1. FLAG-hIes2

   Plasmid

   #15355

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   PAP-1 binding protein (INO80B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

2. PD-L1-miSFIT-Ce67

   Plasmid

   #124686

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-L1 (CD274 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HiBiT-FZD10

   Plasmid

   #195848

   Purpose

   Expresses human FZD10 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD10 (FZD10 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

   Plasmid

   #218864

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8s
   

   Use

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   S26M F286Y Q315H

   Promoter

   hsp70

   Available since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. single polypeptide FPX biosensor for caspase-8

   Plasmid

   #60884

   Purpose

   FPX biosensor for caspase-8

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for caspase-8
   

   Use

   Tags

   HindIII site and NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pSS1 (hLPL-FLAG)

   Plasmid

   #102447

   Purpose

   Expresses FLAG-tagged human LPL in mammalian cells

   Depositor

   Brandon Davies

   Article

   Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

   Insert

   Lipoprotein lipase (LPL Human)
   

   Use

   Tags

   6x His and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSP64-hKCNQ1

   Plasmid

   #53048

   Purpose

   Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

   Insert

   Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-lox-3xSTOP-lox (JDW 869)

   Plasmid

   #224521

   Purpose

   A Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LoxP-3xStop-LoxP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTet-GLI2shR

   Plasmid

   #136691

   Purpose

   Inducible Gli2 shRNA lentiviral plasmid (target sequence: human GLI2 5′CCGGCCTGGCATGACTACCACTATGCTCGAGCATAGTGGTAGTCATGCCAGGTTTTTG 3′)

   Depositor

   LuZhe Sun

   Article

   Zhu et al Lab Invest. 2018 Nov;98(11):1384-1396. d

   Insert

   shRNA_humanGli2 (GLI2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYLsgRNA-OsU3m/LacZ

    Plasmid

    #66192

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    OsU3m (SpeI site destroyed)

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX304_zeo

    Plasmid

    #160092

    Purpose

    Lentiviral vector for mammalian gene expression

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PD-1-miSFIT-1x

    Plasmid

    #124679

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Ef1a-DIO hChR2(E123A)-EYFP

    Plasmid

    #35507

    Purpose

    AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_RBMX-eA3A-UdgX-HF-nCas9

    Plasmid

    #163548

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-HF-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHS18 (mANGPTL3-Strep)

    Plasmid

    #109111

    Purpose

    Expresses StrepII-tagged mouse ANGPTL3 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL3 (Angptl3 Mouse)
    

    Use

    Tags

    StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE)

    Plasmid

    #214878

    Purpose

    Lentiviral vector encoding RfxCas13d targeting SURF1 guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crSURF1-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CaMKIIa-hChR2(E123A)-mCherry

    Plasmid

    #35506

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Has Service

    AAV9

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV_UdgX-Anc689-UdgX-nCas9-RBMX

    Plasmid

    #163552

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-Anc689-UdgX-nCas9-RBMX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pME-mScarlet-I-P2A-iCre (JDW 917)

    Plasmid

    #224516

    Purpose

    A Gateway-compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet-I-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CaMKIIa-hChR2(E123A)-EYFP

    Plasmid

    #35505

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Has Service

    AAV9

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR221_eGFP (open)

    Plasmid

    #160094

    Purpose

    Expresses eGFP in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    eGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MSCV-human CSF-1R-Y969F,S301-IRES—GFP

    Plasmid

    #115524

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    human CSF-1 receptor constitutively activated mutant (CSF1R Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Y969F,S301

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-FLAG-MTOR-T1977R

    Plasmid

    #69011

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-T1977R (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Threonine 1977 to Arginine

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pKD279.8

    Plasmid

    #173171

    Purpose

    Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

    Depositor

    Jeffrey Tabor

    Article

    Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

    Inserts

    SO_4388(REC)-PsdR(DBD) 137
    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23108 and PpsdA110

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. T7-VEE-CFP

    Plasmid

    #193357

    Purpose

    Self-replicating RNA vector expressing CFP fluorescent protein + PuroR

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    CFP
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA flag-Msi1

    Plasmid

    #184029

    Purpose

    Msi1 clone with N-terminal flag tag

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Msi1 (Msi1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AICSDP-55:DMD-mEGFP

    Plasmid

    #159744

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Insert

    DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pME-Lifeact-EGFP-3xHA (JDW 1322)

    Plasmid

    #224494

    Purpose

    Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Liefact-EGFP-3xHA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TOP2A sgRNA1

    Plasmid

    #138190

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TOP2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TOP2A sgRNA2

    Plasmid

    #138191

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TOP2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HiBiT-FZD5

    Plasmid

    #195843

    Purpose

    Expresses human FZD5 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD5 (FZD5 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCS-CG-WT-Cer-VWF

    Plasmid

    #124827

    Purpose

    Expresses multimeric human VWF-ECFP fusion protein. The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.

    Depositor

    Sriram Neelamegham

    Article

    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

    Insert

    VWF with cyan fluorescent protein fusion between D'D3 and A1 domains (VWF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mCardinal-H2B-C-10

    Plasmid

    #56162

    Purpose

    Localization: Nucleus/Histones, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

34. AAV_Actb HMEJ donor

    Plasmid

    #97318

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid

    #218867

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8f
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEB3-miRFP670nano

    Plasmid

    #127433

    Purpose

    EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    EB3-miRFP670nano (MAPRE3 Synthetic, Human)
    

    Use

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-Tag2-Sox9 T236A

    Plasmid

    #111455

    Purpose

    Transient expression of Flag-tagged human Sox9 with T236A mutation

    Depositor

    Sharon Pine

    Article

    Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

    Insert

    Sox9 (SOX9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T236A mutation

    Promoter

    CMV

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-FLAG-MTOR-F1888L

    Plasmid

    #69010

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-F1888L (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Phenylalanine 1888 to Leucine

    Promoter

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

39. mCardinal-Tubulin-C-18

    Plasmid

    #56176

    Purpose

    Localization: Microtubules, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

40. pBMN Parkin

    Plasmid

    #89299

    Purpose

    For expression of untagged Parkin

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge