215,785 results

1. pLVX-Puro-hGSDME

   Plasmid

   #154876

   Purpose

   Expresses hGSDME in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (GSDME Human)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. actc1b-2PH-PLCd-mKate2

   Plasmid

   #109500

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-2PH-PLCd-mKate2
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Butelase AY

   Plasmid

   #221847

   Purpose

   Expresses Butelase AY with Val237Ala and Thr238Tyr mutations

   Depositor

   Artur Krężel

   Article

   Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

   Insert

   Butelase 1 ligase
   

   Expression

   Bacterial

   Mutation

   Val237Ala, Thr238Tyr

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. M43: pHR-SFFVp-iLID::EGFP::FTH1

   Plasmid

   #122149

   Purpose

   Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FTH1 (FTH1 Human)
   

   Use

   Lentiviral

   Tags

   NLS-iLID-GFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiGuide-mCherry

   Plasmid

   #217005

   Purpose

   lentiviral plasmid for gRNA cloning with selectable mCherry expression

   Depositor

   Deepta Bhattacharya

   Article

   D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-myc3-RBBP4

   Plasmid

   #20715

   Depositor

   Yue Xiong

   Article

   He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

   Insert

   retinoblastoma binding protein 4 (RBBP4 Human)
   

   Tags

   myc3

   Expression

   Mammalian

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-myc3-RBBP4

   Plasmid

   #20715

   Depositor

   Yue Xiong

   Article

   He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

   Insert

   retinoblastoma binding protein 4 (RBBP4 Human)
   

   Tags

   myc3

   Expression

   Mammalian

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hy_pMT Flag-UAP56 WT

   Plasmid

   #110125

   Purpose

   Expresses Flag-tagged human UAP56 (DDX39B)

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   UAP56 (DDX39B Human)
   

   Tags

   Flag

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.3_SOX2

   Plasmid

   #26817

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

   Insert

   5'UTR-SOX2-3'UTR (SOX2 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA3.3_SOX2

    Plasmid

    #26817

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-SOX2-3'UTR (SOX2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pLenti.CAG.H2B-cerFP.W.SINloxP

    Plasmid

    #51006

    Purpose

    Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-cerFP
    

    Use

    Lentiviral

    Tags

    histone 2B

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti.CAG.H2B-cerFP.W.SINloxP

    Plasmid

    #51006

    Purpose

    Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-cerFP
    

    Use

    Lentiviral

    Tags

    histone 2B

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-ISceID44A

    Plasmid

    #59424

    Purpose

    Expresses a catalytically inactive form of the endonuclease ISceI

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    endonuclease ISceI mutant (I-SceI )
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed aspartate 44 to alanine (D44A)

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-ISceID44A

    Plasmid

    #59424

    Purpose

    Expresses a catalytically inactive form of the endonuclease ISceI

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    endonuclease ISceI mutant (I-SceI )
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed aspartate 44 to alanine (D44A)

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCKmatBC

    Plasmid

    #138587

    Purpose

    matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    matB contains native GUG start codon

    Promoter

    Plac promoter

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR GFP-CaaX

    Plasmid

    #113020

    Purpose

    for lentivirus production of GFP-CaaX

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    GFP-CaaX
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T-1 3xFlag-JNK1a1

    Plasmid

    #47574

    Purpose

    Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    JNK1a1 (MAPK8 Human)
    

    Tags

    3xFlag and GST

    Expression

    Bacterial

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pGEX-4T-1 3xFlag-JNK1a1

    Plasmid

    #47574

    Purpose

    Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    JNK1a1 (MAPK8 Human)
    

    Tags

    3xFlag and GST

    Expression

    Bacterial

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pEGFP-C1-hAtg2A

    Plasmid

    #36456

    Depositor

    Noboru Mizushima

    Article

    Velikkakath et al Mol Biol Cell. 2012 Mar;23(5):896-909. E

    Insert

    autophagy-related protein 2 homolog A (ATG2A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed Proline 656 to Arginine

    Promoter

    CMV

    Available Since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1-hAtg2A

    Plasmid

    #36456

    Depositor

    Noboru Mizushima

    Article

    Velikkakath et al Mol Biol Cell. 2012 Mar;23(5):896-909. E

    Insert

    autophagy-related protein 2 homolog A (ATG2A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed Proline 656 to Arginine

    Promoter

    CMV

    Available Since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-TRE-mRuby2

    Plasmid

    #99114

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mRuby2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pmCherry-T2A-moxMaple3

    Plasmid

    #120875

    Purpose

    Mammalian expression of mCherry and moxMaple3

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

    Inserts

    mCherry
    moxMaple3
    

    Expression

    Mammalian

    Mutation

    from mMaple3, moxMaple3 was engineered by replaci…

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pEGFP-Merlin S518A

    Plasmid

    #84294

    Purpose

    Expression of Merlin with an alanine mutation at the Pak phosphorylation site

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S518A

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLenti-hSyn-PROGERIN

    Plasmid

    #140189

    Purpose

    Overexpression of Progerin with a GFP fusion protein, under human synapsin promotor

    Depositor

    Lorenz Studer

    Article

    Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

    Insert

    Progerin (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pYeDP60-hATP13A2-WT

    Plasmid

    #171377

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Tags

    BAD tag

    Expression

    Yeast

    Promoter

    URA3

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pDEST26-PPP2CB-C-HA

    Plasmid

    #195179

    Purpose

    Vector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloning

    Depositor

    Stephanie Roessler

    Insert

    PPP2CB (PPP2CB Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FLX1.8NFATGFPpd2HS4

    Plasmid

    #169096

    Purpose

    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

    Depositor

    Geoffrey Owens

    Inserts

    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    

    Use

    Lentiviral

    Promoter

    NFAT enhancer + synthetic TATA box

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLPC-dnMAML1-mVenus

    Plasmid

    #171023

    Purpose

    Retroviral vector for CMV promoter driven expression of dominant negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).

    Depositor

    Masashi Narita

    Article

    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

    Insert

    MAML1 Dominant Negative (MAML1 Human)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    amino acids 12-74 only, hence conferring dominant…

    Promoter

    CMV

    Available Since

    April 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro

    Plasmid

    #210857

    Purpose

    Lentiviral expression vector encoding FIP200 mutant-mCherry

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Mutation

    V44D/V314D/L324D/R543D/R548D

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. MGP M155

    Plasmid

    #26527

    Depositor

    David Baltimore

    Article

    O'Connell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 1

    Insert

    miR-155 (Mir155 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

31. MGP M155

    Plasmid

    #26527

    Depositor

    David Baltimore

    Article

    O'Connell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 1

    Insert

    miR-155 (Mir155 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

32. pET-28a(+)-T7-OmpA-MNaseA-6xHis

    Plasmid

    #214808

    Purpose

    Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

    Depositor

    Peter Sarin

    Article

    Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

    Insert

    OmpA-Micrococcal nuclease A
    

    Tags

    6xhis

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV BiP-Myc-KDEL-wt

    Plasmid

    #27164

    Purpose

    Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV BiP-Myc-KDEL-wt

    Plasmid

    #27164

    Purpose

    Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pML104-KanMx4

    Plasmid

    #83476

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    KanMX4
    

    Expression

    Yeast

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. VGluT1-SNAPtag

    Plasmid

    #107371

    Purpose

    Synaptic vesicle protein VGluT1 fused to the SNAPtag domain

    Depositor

    David Perrais

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    Vesicular Glutamate transporter 1 (Slc17a7 Rat)
    

    Tags

    SNAPtag, 104 aa before C term

    Expression

    Mammalian

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

    Plasmid

    #179116

    Purpose

    Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    Cargo EGFP with AttP Bxb1 site for in-frame fusion
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pM2s2AsG

    Plasmid

    #137924

    Purpose

    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    Superfolder GFP
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Expression

    Bacterial

    Promoter

    J23101

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLKO.1 - TRC mTagBFP2

    Plasmid

    #191566

    Purpose

    Expresses an shRNA and mTagBFP2

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 for shRNA

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pME phluorin2-gpi

    Plasmid

    #73793

    Purpose

    ratiometric pHluorin2 with a gpi linkage sequence in a middle entry clone

    Depositor

    David Raible

    Article

    Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

    Insert

    ratiometric pHluorin2-gpi
    

    Use

    Middle entry clone

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge