167,785 results

1. gRNA_Cloning Vector BbsI

   Plasmid

   #128433

   Purpose

   Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing

   Depositor

   Izuho Hatada

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAV-U6+27-Tornado-RhoBAST

   Plasmid

   #196341

   Purpose

   Overexpression of circular RhoBAST

   Depositor

   Murat Sunbul

   Article

   Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

   Insert

   Tornado-RhoBAST
   

   Expression

   Mammalian

   Promoter

   U6+27

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX458-Dual-Guide-Donor-ShuttleOnly

   Plasmid

   #171098

   Purpose

   Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Mutation

   XbaI site added upstream of U6 promotor. GtoC mu…

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX6P1-human LIC1 C-terminal half

   Plasmid

   #74599

   Purpose

   used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
   

   Tags

   N-terminal GST and Strep II

   Expression

   Bacterial

   Mutation

   amino acids 389-523 of LIC1

   Promoter

   T7

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTYB2-eIF1A

   Plasmid

   #37218

   Depositor

   Jon Lorsch

   Article

   Acker et al Methods Enzymol. 2007;430:111-45.

   Insert

   eIF1A
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

6. cmv-mCherry-10xPP7

   Plasmid

   #185796

   Purpose

   mCherry labeled with 10xPP7

   Depositor

   Robin Lee

   Article

   Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

   Insert

   cmv-mCherry-10xPP7
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HCA2-Tango

   Plasmid

   #66396

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   HCA2 (HCAR2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FKBP-mCherry

   Plasmid

   #108122

   Purpose

   Recruitable fluorophore

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   FKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAS1NB c Rosella I

   Plasmid

   #71245

   Purpose

   a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

   Depositor

   Mark Prescott

   Article

   Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

   Insert

   Rosella
   

   Expression

   Yeast

   Promoter

   PGK1

   Available Since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p404GALS

    Plasmid

    #17435

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    GALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    GALS promoter is between SacI and XbaI restrictio…

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Str-KDEL_TNF-SBP-EGFP

    Plasmid

    #65278

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SuExp His-Myc-hPLD4

    Plasmid

    #173852

    Purpose

    Protein expression plasmid for recombinant His-Myc tag human PLD4

    Depositor

    David Nemazee

    Article

    Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

    Insert

    PLD4 (PLD4 Human)
    

    Tags

    6xHis, Myc, Factor X cleavable

    Expression

    Mammalian

    Mutation

    intracellular and transmembrane domain truncated,…

    Promoter

    CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-α-cateninWT

    Plasmid

    #229709

    Purpose

    Transient expression of GFP-alpha-cateninWT in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOSIP-KH (KanR, HK022)

    Plasmid

    #45983

    Depositor

    Drew Endy, Keith Shearwin

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKL2310

    Plasmid

    #187875

    Purpose

    Empty pVS1-based INTEGRATE vector with loxP cargo

    Depositor

    Kan Wang

    Article

    Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pROSA26-CreERT2

    Plasmid

    #149437

    Purpose

    ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

    Depositor

    Primo Schaer

    Article

    Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

    Insert

    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    ERT2

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB-Rex

    Plasmid

    #122134

    Purpose

    B. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 origin

    Depositor

    Srivatsan Raman

    Article

    Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

    Insert

    B. subtilis Rex
    

    Expression

    Bacterial

    Promoter

    apFAB338

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mARGAna (cassette 1)

    Plasmid

    #191341

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 1)
    

    Expression

    Mammalian

    Promoter

    TRE3GV

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-RGS18HIS-kanMX6

    Plasmid

    #26882

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    18xHis tag
    

    Use

    Pcr tagging yeast

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn1-mNeonGreen

    Plasmid

    #99135

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mNeonGreen
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pmEGFP-C1

    Plasmid

    #36412

    Depositor

    Benjamin Glick

    Type

    Empty backbone

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCW57.1-Tet-UPF1WT (pRKB264)

    Plasmid

    #99146

    Purpose

    Inducible lentiviral construct to express FLAG-tagged UPF1wt

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    First 127 codons are optimized to reduce GC conte…

    Promoter

    pTight

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF-BFP

    Plasmid

    #138272

    Purpose

    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    BFP H66 PAM
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    H66, PAM motif

    Promoter

    Ef1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-MET-V5/HIS

    Plasmid

    #201982

    Purpose

    expression of human MET receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MET receptor tyrosine kinase (MET Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. peGFP C3-SPG20 8xV

    Plasmid

    #218579

    Purpose

    Expresses GFP-tagged human SPG20 bulky hydrophobic amino acid mutant in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Tryptophan 419, Leucine 438, Isoleucine 4…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFA6a-TRP1-PGAL1

    Plasmid

    #41606

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    TRP1 reverse orientation

    Available Since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ANO6-Plvx-mCherry-c1

    Plasmid

    #62554

    Purpose

    Full length mouse ANO6 tagged with mCherry on the C-terminus

    Depositor

    Renzhi Han

    Article

    Zhao et al PLoS One. 2014 Mar 24;9(3):e92749. doi:

    Insert

    mouse ANO6 (Ano6 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Promoter

    CMV

    Available Since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLX303

    Plasmid

    #25897

    Purpose

    3rd generation lentiviral Gateway destination vector. Blasticidin selection.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Expression

    Mammalian

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FUW-TetO-lox-ERAS

    Plasmid

    #52417

    Purpose

    Doxycycline inducible lentiviral vector of human ERAS cDNA with excisable insert

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    ERAS (ERAS Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2 (AAV9)

    Viral Prep

    #164510-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2 (#164510). In addition to the viral particles, you will also receive purified pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2 plasmid DNA. Synapsin-driven, cytosolic-targeted iGlucoSnFR-mRuby2

    Depositor

    Loren Looger

    Article

    Keller et al bioRxiv (2019) 571422; doi: https://doi.

    Promoter

    Syn

    Tags

    mRuby2

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

31. coel::RFP

    Plasmid

    #8938

    Depositor

    Piali Sengupta

    Article

    Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

    Insert

    unc-122 promoter (unc-122 Nematode)
    

    Tags

    RFP

    Expression

    Worm

    Available Since

    Nov. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-TLR3-CFP

    Plasmid

    #13641

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 3 (TLR3 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p206-Switch-ON

    Plasmid

    #217885

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAG-mRuby3

    Plasmid

    #171154

    Purpose

    Encodes mRuby3 fluorophore

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    mRuby3
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Calreticulin-SuperNova-KDEL

    Plasmid

    #131857

    Purpose

    Expresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signal

    Depositor

    Geert van den Bogaart

    Article

    Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

    Insert

    SuperNova
    

    Tags

    KDEL ER-retention signal and Signal sequence of h…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDS24

    Plasmid

    #58952

    Purpose

    Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

    Depositor

    Thomas Fox

    Article

    Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93

    Insert

    Mitochondrially coded Arg8 protein
    

    Mutation

    Coding sequence synthetic in S. cerevisiae mitoch…

    Promoter

    n/a

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pLARRY-EGFP

    Plasmid

    #140025

    Purpose

    Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

    Depositor

    Fernando Camargo

    Article

    Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

    Insert

    EGFP
    

    Use

    Lentiviral

    Promoter

    EF1alpha

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEB2-mKate2

    Plasmid

    #104009

    Purpose

    Plasmid encoding mKate2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mKate2
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, V45A, …

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBAD18HMGR

    Plasmid

    #19041

    Depositor

    Jay Keasling

    Article

    Pitera et al Metab Eng. 2007 Mar . 9(2):193-207.

    Insert

    truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (HMG1 Budding Yeast)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

40. CbS-Flag-TRRAP

    Plasmid

    #32103

    Depositor

    Yardena Samuels, Michael Cole

    Insert

    Transformation/transcription domain-associated protein (TRRAP Human)
    

    Tags

    Flag 1x

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge