169,069 results

1. TALED_Left-ND1-AD-E1347A

   Plasmid

   #183894

   Purpose

   Expresses TALED_Left-ND1-AD-E1347A in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   TALED for ND1 mutation
   

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1_3XFlag

   Plasmid

   #182494

   Purpose

   Empty plasmid containing Flag tag for C-terminal tagging

   Depositor

   Paolo Comoglio

   Article

   Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

   Insert

   3xFlag with 5 Glycolic spacer
   

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSNAP-tag (m) Vector

   Plasmid

   #101135

   Purpose

   Expression of protein of interest with SNAP-Tag in Mammalian cells

   Depositor

   New England Biolabs, Ana Egana

   Type

   Empty backbone

   Tags

   SNAP-tag (SNAP26m)

   Expression

   Mammalian

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. hsgk1

   Plasmid

   #83432

   Purpose

   full length human sgk1 with Flag tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

   Insert

   human Sgk1 (SGK1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TREtight-mTagBFP2-B19G (AAV1)

   Viral Prep

   #100799-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-TREtight-mTagBFP2-B19G (#100799). In addition to the viral particles, you will also receive purified pAAV-TREtight-mTagBFP2-B19G plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA (Addgene# 100798). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ian Wickersham

   Article

   Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

   Promoter

   TREtight

   Tags

   BFP (in presence of Cre and 100798)

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pcDNAHygro(+)LAMP2AS

   Plasmid

   #86146

   Purpose

   Anti sense for LAMP2

   Depositor

   Janice Blum

   Article

   Zhou et al Immunity. 2005 May;22(5):571-81.

   Insert

   LAMP2a (LAMP2 Human)
   

   Expression

   Mammalian

   Mutation

   contains first 525 bases of LAMP cDNA

   Promoter

   CMV

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV4 p65

   Plasmid

   #21966

   Depositor

   Warner Greene

   Article

   Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

   Insert

   NFkB p65 subunit (RELA Human)
   

   Expression

   Mammalian

   Mutation

   This construct was the product of 3 subcloning pr…

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX4T-1 human RNF146 WWE (99-183)

   Plasmid

   #132613

   Purpose

   expresses human RNF146 WWE (99-183) in E. coli

   Depositor

   Wenqing Xu

   Article

   Wang et al Genes Dev. 2012 Feb 1;26(3):235-40. doi:

   Insert

   RNF146 WWE (99-183)
   

   Expression

   Bacterial

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Orai3

   Plasmid

   #16370

   Depositor

   Anjana Rao

   Article

   Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

   Insert

   Orai3 (ORAI3 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139093

    Purpose

    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dxCas9-NG-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. FRP2371

    Plasmid

    #127577

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Expression

    Yeast

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Rap1A FLARE

    Plasmid

    #134926

    Purpose

    Rap1A biosensor

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    Rap1A, mCerulean3, RaIGDS RBD, YPet
    

    Expression

    Mammalian

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AiP13999 - pAAV-AiE0387m-minBG-iCre(R297T)-BGHpA (Alias: CN3999)

    Plasmid

    #208166

    Purpose

    AiE0387m is an enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCreR297T
    

    Use

    AAV

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b-ptetO::bac::gfp

    Plasmid

    #217882

    Purpose

    expresses the bac-BGC in bacterial cells, produces bacillamide D

    Depositor

    Tobias A. M. Gulder

    Article

    Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

    Inserts

    Dehydrogenase
    Non-ribosomal peptide synthetase
    Aminotransferase
    

    Expression

    Bacterial

    Promoter

    ptetO

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Mouse sgRNA library Brie in lentiCRISPRv2

    Pooled Library

    #73632

    Purpose

    Mouse sgRNA library in backbone lentiCRISPRv2 containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3 Rod Opsin

    Plasmid

    #109361

    Purpose

    Human rod opsin

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin (RHO Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_BMP2_WT

    Plasmid

    #82101

    Purpose

    Gateway Donor vector containing BMP2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    BMP2 (BMP2 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. HDAC6 Flag

    Plasmid

    #13823

    Purpose

    Mammalian expression of human histone deacetylase 6 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC6 (HDAC6 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1+-Tornado-nLuc

    Plasmid

    #212611

    Purpose

    For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    Tornado-CVB3-nLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Rap1B FLARE

    Plasmid

    #134927

    Purpose

    Rap1B biosensor

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet, Cerulean, Rap1B
    

    Expression

    Mammalian

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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