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mNeonGreen-APEX3

Plasmid

#202590

Purpose

Gamma-retroviral vector that expresses mNeonGreen fused to APEX3.

Depositor

Reuben Harris

Article

Becker et al J Mol Biol. 2023 May 12:168145. doi: 10.

Insert

mNeonGreen-APEX3-V5 (APX1 )

Use

Retroviral

Tags

V5 epitope and mNeonGreen

Expression

Mammalian

Mutation

APEX2 L242A

Promoter

MESV LTR

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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VKG1-gRNA-pX330

Plasmid

#108671

Purpose

Cleavage of VKG1 sequence by CRISPR/Cas9

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

gRNA for VKG1 sequence

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4-GFP-TEV-B55

Plasmid

#217378

Purpose

expresses GFP tagged B55 in mammalian cells; tev cleavable

Depositor

Wolfgang Peti

Article

Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

Insert

B55 (PPP2R2A Human)

Use

Tags

EGFP TEV

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNCS mOrange

Plasmid

#91764

Purpose

Bacterial expression of a fluorescent protein, monomeric Orange (mOrange)

Depositor

Roger Tsien, Erik Rodriguez

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Orange

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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GEPIR Sox4.2091

Plasmid

#101118

Purpose

shRNA vec

Depositor

Joan Massague

Article

David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

Insert

Sox4.2137

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only
pVKG1-Puro

Plasmid

#108670

Purpose

Puromycin resistant cassette for knock-in by VIKING method

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

PURO

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-TRE3G-LbNOX-Flag

Plasmid

#234980

Purpose

Expresses LbNOX in mouse cell lines

Depositor

Denis Titov

Article

Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

Insert

LbNOX

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-TRE3G-mitoLbNOX-Flag

Plasmid

#234981

Purpose

Expresses mitochondrial LbNOX in mouse cell lines

Depositor

Denis Titov

Article

Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

Insert

mito-LbNOX

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_rd6_pegRNA_(PP7-C4-Q1)

Plasmid

#232433

Purpose

pegRNA with optimized 3' modifications to correct the rd6 mutation

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK066 [Con-Pro_nptII-Broad_PartType8]

Plasmid

#229303

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_nptII-Broad_PartType8

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Insert

nptII Promoter (Broad)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBCG10

Plasmid

#232323

Purpose

Make mazF a counterselection marker to replace sacB in pZP06C

Depositor

Chenggang Wu

Insert

mazF-tetR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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fabp10a:GCaMP6s; cryaa:mCherry

Plasmid

#230060

Purpose

Expression of genetically encoded calcium indicator in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Pozo-Morales et al Hepatology. 2023 Mar 1;77(3):789-801. do

Insert

GCaMP6s

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

fabp10a

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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LCV2 control

Plasmid

#217443

Purpose

Lentiviral vector expressing Cas9 without a targeting sgRNA

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_coGag

Plasmid

#212656

Purpose

piggyBac vector to express codon optimized MLV Gag

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCHC039 (∆phcA)

Plasmid

#226207

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for PhcA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUb-G76V-GFP-IRES-DsRed

Plasmid

#225503

Purpose

Expression of Ub-G76V-GFP along dsRED from one mRNA

Depositor

David Rubinsztein

Article

Yu et al Hum Mol Genet. 2024 May 22:ddae085. doi:

Insert

Ub-G76V-GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Promoter

CMV promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet3-Giantin (JDW 1356)

Plasmid

#224502

Purpose

Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

mScarlet3-Giantin

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-GFP-LA

Plasmid

#206198

Purpose

Expresses GFP-tagged Lamin-A specifically in cardiomyocytes by the Tnnt2 promoter

Depositor

Yuxuan Guo

Article

Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

Insert

Lmna (LMNA Human)

Use

AAV

Tags

GFP

Expression

Mutation

Promoter

Tnnt2

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1-mScarlet

Plasmid

#211330

Purpose

Mammalian overexpression vector for C-terminally mScarlet tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2527

Plasmid

#142396

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ERCC3 (ERCC3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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