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Plasmid#109909PurposeProtein expression and purification of ESRRB_Hormone-recepDepositorInsertESRRB_Hormone-recep (ESRRB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-NLS-mRuby2 (AAV1)
Viral Prep#99130-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-mDlx-NLS-mRuby2 (#99130). In addition to the viral particles, you will also receive purified pAAV-mDlx-NLS-mRuby2 plasmid DNA. mDlx-driven NLS-mRuby2 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromotermDLX5/6TagsmRuby2Available sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE
Plasmid#51502PurposeCan be used to generate AAV virus that will express EGFP in the presence of CreDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-IL1RAPL1-Fc(DAPA)-AviTag-6xHis
Plasmid#156778PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertIL1RAPL1 (IL1RAPL1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex_307-ccdB-hygro
Plasmid#201143PurposepLEX_307 plasmid with hygro markerDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-pBAD
Plasmid#54844PurposeLocalization: Protein Expression Vector , Excitation: 434, Emission: 474DepositorTypeEmpty backboneUseTags6x His, T7 tag, and TEV siteExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFLAG_G495R MDA5
Plasmid#216799PurposeExpresses a single point mutant of melanoma differentiation-associated protein 5DepositorInsertmelanoma differentiation-associated protein 5 (IFIH1 Human)
UseTagsExpressionMammalianMutationG495RPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit
Plasmid Kit#1000000210PurposeToolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.DepositorAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-7xGFP11
Plasmid#138397PurposeRMCE vector for generating C-terminal fusion of seven tandem GFP11 tags (split GFP)DepositorInsert7xGFP11
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
Plasmid#140082PurposeEntry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoterDepositorInsertpT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
UseIn vitro transcription (mrna synthesis)TagsExpressionBacterialMutationPromoterT7Available sinceOct. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC4A2_STOP
Plasmid#161442PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC4A2 (SLC4A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTRETightBI-RY-0
Plasmid#31463DepositorInserteYFP and mCherry
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS415-GPD
Plasmid#183940PurposeControl vector that has a GPD promoter with no insertDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA
Plasmid#65454PurposeCan be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinasesDepositorInsertChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMutationH134R variantPromoterhSyn1Available sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Eptesicus fuscus
Plasmid#78113PurposeNt His-tag Eptesicus fuscus Sacsin 89-1368DepositorInsertSacsin Eptesicus fuscus 89-1368
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
GRM2-DuET
Plasmid#213301PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGRM2 (GRM2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAT527
Plasmid#180514PurposePlasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-M2rtTA
Plasmid#60843PurposeAAVS1 donor vector for genomic targetingDepositorInsertsM2rtTA
Neo
UseTargeting donorTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_LC_WT
Plasmid#98653PurposeExpresses MBP-tagged FUS AA 1-163DepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-GLUT5 in pRK5
Plasmid#154205PurposeN-terminal HA-tagged GLUT5 cDNA expression vector for transfectionsDepositorInsertSLC2A5 (SLC2A5 Human)
UseTagsN terminal HA tagExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDG_GRID1_OE_1
Plasmid#161661PurposeOverexpresses GRID1DepositorInsertGRID1 (GRID1 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAC-TFRC
Plasmid#172420PurposeMAC-tagged gene expressionDepositorInsertTransferrin receptor protein 1 (TfR) (CD antigen CD71) (TFRC Human)
UseTagsMAC-tagExpressionMammalianMutationPromoterCMV promoterAvailable sinceNov. 4, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWZL-Neo-Myr-Flag-DEST
Plasmid#15300Purposea Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORFDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
Halo-GPI-RUSH
Plasmid#166903PurposeRUSH cargo: Str-KDEL_IRES_SBP-Halo-GPIDepositorInsertHaloTag
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pc3.1-CAGGS-ASAP3-mCyRFP3
Plasmid#193323PurposeA red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3DepositorInsertASAP3-R3
UseTagsExpressionMammalianMutationPromotercaggsAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL023_Tub-rb_IgG_HC
Plasmid#193620Purposeα-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.DepositorInsertAnti-Tubulin scFv heavy variable + rabbit constant chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSL21-VEX
Plasmid#158230PurposeRetroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)DepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSFS2A-CaHygB
Plasmid#189564PurposeCaHygB hygromycin resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicansDepositorInsertHygB
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-ecfp
Plasmid#78463PurposeBroad range bacterial fluorescence labelingDepositorInserteCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-KIAA1967
Plasmid#38082DepositorInsertKIAA1967 (CCAR2 Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC6A3_STOP
Plasmid#161326PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC6A3 (SLC6A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
eGFP STAT1 S727A
Plasmid#12304DepositorInsertSTAT1 S727A (STAT1 Human)
UseTagseGFPExpressionMammalianMutationS727APromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJL-TRBO
Plasmid#80082PurposeAn improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT
Plasmid#207832Purposetelomere specific nuclease to create double-strand breaks at telomeresDepositorInsertTRF1 (TERF1 Human)
UseLentiviralTagsFlag, DD, ER, mCherry and FokIExpressionMammalianMutationPromoterTRE3GAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFLP-hyg
Plasmid#87831PurposeExpresses FLP recombinase with hyg selection markerDepositorInserthyg
UseTagsNoneExpressionBacterialMutationNonePromoterAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
LT1_33_pTEFmut8-mCherry
Plasmid#177706PurposeConstruct for genome integration expressing constitutively mCherry under pTEFmut8 promoterDepositorInsertpTEFmut8-mCherry
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAJM.474
Plasmid#108526PurposeLuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.DepositorInsertLuxR + PLuxB-YFP
UseTagsExpressionBacterialMutationVanR: T49I A111V P179SPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR3-CFP
Plasmid#13641DepositorInsertToll-Like Receptor 3 (TLR3 Human)
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceJune 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
Alpha-Synuclein-His
Plasmid#186802PurposeCodon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.DepositorInsertAlpha-Synuclein-His (SNCA Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only