197,403 results

1. pHR-tdPP7-3xmCherry

   Plasmid

   #74926

   Purpose

   Expresses tdPP7-3xmCherry

   Depositor

   Marvin Tanenbaum

   Article

   Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

   Insert

   PP7-3xmCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_hSyn-PdCO-EGFP-WPRE (AAV1)

   Viral Prep

   #198513-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV_hSyn-PdCO-EGFP-WPRE (#198513). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-EGFP-WPRE plasmid DNA. Synapsin-driven expression of the optimized opsin PdCO in frame with EGFP. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Promoter

   Syn

   Tags

   EGFP

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

3. pLminP_Luc2P_RE3

   Plasmid

   #90337

   Purpose

   AHR - Aryl hydrocarbon receptor gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   AHR

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-PB-SRT-tdTomato

   Plasmid

   #154889

   Purpose

   piggyBac self-reporting transposon with tdTomato marker in AAV backbone

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   piggyBac SRT
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PRS303-240xtetO

   Plasmid

   #44754

   Depositor

   Narendra Maheshri

   Article

   Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

   Insert

   240x tetO array and CHRI homology
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42337

   Purpose

   Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-CL7-2NLS-SpyCas9(dS)-A22p3star

   Plasmid

   #222970

   Purpose

   Bacterial expression of A22p-fused SpyCas9 construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

   Insert

   His-CL7-2NLS-SpyCas9(dS)-A22p3
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4-M2L

   Plasmid

   #20926

   Purpose

   used in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28

   Depositor

   James Thomson

   Article

   Yu et al Science. 2009 Mar 26.

   Inserts

   MYC (MYC Human)
   LIN28 (LIN28A Human)
   

   Use

   Episomal/ebna

   Tags

   Expression

   Mutation

   Promoter

   CMV and IRES

   Available since

   May 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTA7002-avrPto

   Plasmid

   #49156

   Purpose

   Plant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPto

   Depositor

   Sheng Yang He

   Article

   Hauck et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

   Insert

   avrPto
   

   Use

   Plant transformation binary vector

   Tags

   Expression

   Mutation

   Promoter

   dexamethasone inducible

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pET28_6xHis-PARP1delBRCT

    Plasmid

    #173938

    Purpose

    Bacterial expression of PARP1 lacking BRCT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delBRCT (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 374-520; V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. WFS1-Flag-pcDNA3

    Plasmid

    #13011

    Depositor

    Fumihiko Urano

    Article

    Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

    Insert

    Wolfram Syndrome 1 (WFS1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPomyces-FnCpf1

    Plasmid

    #129554

    Purpose

    Contains codon-optimized FnCpf1 and gRNA for streptomyces

    Depositor

    Mingzi M. Zhang, Fong Tian Wong

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    FnCpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. miniCGBE1 (pBM1219)

    Plasmid

    #140253

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in Cas9

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBB572

    Plasmid

    #27397

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 . 7():32.

    Insert

    ibpA, ibpB (ibpB, ibpA E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54

    Plasmid

    #111727

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q54 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-IQGAP1

    Plasmid

    #30112

    Depositor

    David Sacks

    Article

    Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

    Insert

    IQGAP1 (IQGAP1 Human)
    

    Use

    Tags

    tandem EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAB1678 CMV-HIVNES-GS-Cas13bt1

    Plasmid

    #176316

    Purpose

    CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt1
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF2053

    Plasmid

    #142912

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BCL6 (BCL6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PKC eta WT

    Plasmid

    #21244

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC eta (Prkch Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pCW57.1-DUX4-WT

    Plasmid

    #99282

    Purpose

    All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)

    Depositor

    Stephen Tapscott

    Article

    Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEGFP_mNG2(11)_Clathrin light chain

    Plasmid

    #82608

    Purpose

    Expresses mNG2(11) tagged clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNG2(11)_Clathrin light chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. qTAG-C-mStayGold-Zeo

    Plasmid

    #227255

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDH-anti-mCherry(LaM6) PAGER(Gq)

    Plasmid

    #230016

    Purpose

    Lentiviral vector expressing anti-mCherry PAGER(Gq) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM1D
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    W442A on hM1D

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCDH-anti-mCherry(LaM6) PAGER(Gs)

    Plasmid

    #230017

    Purpose

    Lentiviral vector expressing anti-mCherry PAGER(Gs) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM1Dxs
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #223173

    Purpose

    Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hfCas13d (N2V8)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TFORF3130

    Plasmid

    #144606

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    IRF8 (IRF8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 1227 pcDNA3 Myr HA Akt2

    Plasmid

    #9016

    Depositor

    William Sellers

    Insert

    Akt2 (AKT2 Human)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET42b-BE3

    Plasmid

    #87437

    Purpose

    Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    BE3 (Apobec1 Bacillus phage PBS2, Rat, Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSP64-hKCNE1

    Plasmid

    #53049

    Purpose

    Expresses beta subunit for IKs channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

    Insert

    Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDule2-Mb haloTyrRS C6

    Plasmid

    #160378

    Purpose

    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

    Depositor

    Ryan Mehl

    Article

    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

    Insert

    C6 HaloTyrosine tRNA synthatase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    L270S, Y271L, N311G, C313T

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. dCas9-dMSK1

    Plasmid

    #165602

    Purpose

    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)

    Depositor

    Isaac Hilton

    Article

    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

    Insert

    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Synthetic, Human, S. Pyogenes)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLOVE-Klf4

    Plasmid

    #15950

    Depositor

    Miguel Ramalho-Santos

    Article

    Blelloch et al Cell Stem Cell. 2007 Sep 13. ():.

    Insert

    Kruppel-like factor 4 (Klf4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    The recombination reaction required to clone &quo…

    Promoter

    Available since

    Nov. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLDLR-Luc (aka: pES7)

    Plasmid

    #14940

    Depositor

    Axel Nohturfft

    Article

    Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    LDLR promoter (LDLR Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP

    Plasmid

    #199360

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-SUMOEu-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBLADE_ONLY_A

    Plasmid

    #168051

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Insert

    Blue light-inducible AraC dimers in Escherichia coli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101**

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDEST17-Cdc34a

    Plasmid

    #18674

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Cdc34A (CDC34 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

37. pCB-2

    Plasmid

    #210388

    Purpose

    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

    Depositor

    Gilles van Wezel

    Article

    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

    Inserts

    dCas9
    CymR
    sgRNA cassette
    

    Use

    CRISPR; Integrative vector for use in streptomyces

    Tags

    Expression

    Mutation

    BsaI-flanked RFP cassette inserted in place of sp…

    Promoter

    SP11 and SP30

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pICSL11015

    Plasmid

    #117499

    Purpose

    FAST-Red selectable marker Golden Gate Level 1 Position 1

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
    

    Use

    Tags

    RFP

    Expression

    Plant

    Mutation

    Promoter

    AtOLE1

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPSint

    Plasmid

    #149421

    Purpose

    Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the IV2 intron in the GFP reporter gene.

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

    Plasmid

    #196192

    Purpose

    human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
    

    Use

    Znf donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge