169,069 results

1. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

   Plasmid

   #69546

   Purpose

   Tet-On system expressing MyoD-2A-GFP-2xNLS.

   Depositor

   Charles Gersbach

   Inserts

   eGFP
   Myod1 (Myod1 Mouse)
   

   Use

   Lentiviral

   Tags

   2x nuclear localization sequence and T2A "sk…

   Mutation

   Removed stop codon to fuse it to the 2A-eGFPnls i…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRC-CMV-Rev1b

   Plasmid

   #204153

   Purpose

   Lentivirus helper plasmid coding for Rev

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   Rev
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTcI-Wasabi

   Plasmid

   #86101

   Purpose

   mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TcI
   mWasabi
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   pR/pL and pR/pL, LacI

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEBTet-SNAP-Cep131

   Plasmid

   #136826

   Purpose

   Mammalian expression of the centrosomal protein Cep131 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep131 (CEP131 Human, Synthetic)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.GfaABC1D.GluSnFr.SV40

   Plasmid

   #100889

   Purpose

   AAV expression of GluSnFR, a glutamate sensor

   Depositor

   Baljit Khakh

   Insert

   GluSnFr
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   GfaABC1D

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFPxm163

   Plasmid

   #193480

   Purpose

   Turn-off fluorescent sensor for chloride

   Depositor

   Sheel Dodani

   Article

   Peng et al Chem Sci. 2022 Oct 6;13(43):12659-12672.

   Insert

   GFPxm163
   

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-45b-mCherry

   Plasmid

   #145279

   Purpose

   Bacterial Expression of 6xHis mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   mCherry
   

   Expression

   Bacterial

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_BMP4_WT_V5

   Plasmid

   #82937

   Purpose

   Gateway Donor vector containing BMP4 , used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   BMP4 (BMP4 Human)
   

   Use

   Gateway entry vector

   Mutation

   V152A

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-iRFP670-CaaX

   Plasmid

   #170464

   Purpose

   Expresses iRFP670 fluorophore targeted to the plasma membrane using a prenylation motif

   Depositor

   John James

   Article

   Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

   Insert

   iRFP670-CaaX
   

   Use

   Lentiviral

   Tags

   CaaX

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-CMV-TetO2_mVenus

    Plasmid

    #113893

    Purpose

    Mammalian (inducible) expression of cytosolic mVenus

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    monomeric Venus
    

    Use

    Lentiviral

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. FRP2374

    Plasmid

    #127579

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Expression

    Yeast

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-mEGFP-HMGB1-MUT-Patchless

    Plasmid

    #194553

    Purpose

    Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "Patchless" variant

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    K184Rfs*44, truncation by stop codon after Lys209

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-ChRmine-mScarlet-WPRE

    Plasmid

    #130994

    Purpose

    AAV vector to drive the expression of ChRmine-mScarlet under the control of human synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet

    Promoter

    hSyn

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. GABPA_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176970

    Purpose

    Mammalian expression vector encoding GABPA and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GABPA (GABPA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. mito-BFP

    Plasmid

    #49151

    Purpose

    visualization of mitochondria

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    mitochondrial targeting signal (COX4 Budding Yeast)
    

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiGuide-Hygro-mTagBFP2

    Plasmid

    #99374

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti MS2-P65-HSF1_Hygro

    Plasmid

    #61426

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Insert

    MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1A

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. FNL Combinatorial Cloning Platform

    Plasmid Kit

    #1000000211

    Purpose

    A collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systems

    Depositor

    Dominic Esposito

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

19. kiCAP-AAV-MyoAAV2A

    Plasmid

    #224440

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Mutation

    RGDQTTL insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. mTagRFP-T-Endosomes-14

    Plasmid

    #58014

    Purpose

    Localization: Endosomes, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Endosomes (RHOB Human)
    

    Tags

    mTagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits